DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220166X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro light microscopy light microscopy predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220163X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220164X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220165X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220169X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220167X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220162X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220168X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993) 2011 imex curation Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Stafford RL., Hinde E., Knight MJ., Pennella MA., Ear J., Digman MA., Gratton E., Bowie JU. DIP original experiment: DIP-220170X Structure (London, England : 1993) 2011 IMEx Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. o14936-2 Peripheral plasma membrane protein CASK CASK LIN2 Calcium/calmodulin-dependent serine protein kinase Protein lin-2 homolog protein protein human Homo sapiens Human MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQVLDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAKELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFKIVPSYRTQSSSCERDSPSTSRQSPANGHSSTNNSVSDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNADLVTYEEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPDRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQDISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNEDESLQRLQKESDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY DIP protein O14936-2 original sequence version: 136 cski1_human Caskin-1 CASKIN1 KIAA1306 CASK-interacting protein 1 protein protein human Homo sapiens Human MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELISLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCAALLEPRPGDATDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDSGINAHVRNTYSQTALDIVHQFTTSQASREIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGSRAGTEPSLPQGSSSSGPSAPPEEIWVLRKPFAGGDRSGSISGMAGGRGSGGHALHAGSEGVKLLATVLSQKSVSESGPGDSPAKPPEGSAGVARSQPPVAHAGQVYGEQPPKKLEPASEGKSSEAVSQWLTAFQLQLYAPNFISAGYDLPTISRMTPEDLTAIGVTKPGHRKKIAAEISGLSIPDWLPEHKPANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPTPADCQSPKMTTFQDSELSDELQAAMTGPAEVGPTTEKPSSHLPPTPRATTRQDSSLGGRARHMSSSQELLGDGPPGPSSPMSRSQEYLLDEGPAPGTPPREARPGRHGHSIKRASVPPVPGKPRQVLPPGTSHFTPPQTPTKTRPGSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVEGEVGPAAPGPAPPPVPTAVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSALASANLADEPVPDAEPEDGLLGVRAQCRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPTPRPASPEPGRVATVLASVKHKEAIGPGGEVVNRRRTLSGPVTGLLATARRGPGESADPGPFVEDGTGRQRPRGPSKGEAGVEGPPLAKVEASATLKRRIRAKQNQQENVKFILTESDTVKRRPKAKEREAGPEPPPPLSVYHNGTGTVRRRPASEQAGPPELPPPPPPAEPPPTDLAHLPPLPPPEGEARKPAKPPVSPKPVLTQPVPKLQGSPTPTSKKVPLPGPGSPEVKRAHGTPPPVSPKPPPPPTAPKPVKAVAGLPSGSAGPSPAPSPARQPPAALAKPPGTPPSLGASPAKPPSPGAPALHVPAKPPRAAAAAAAAAAAPPAPPEGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGPRDSAAEKSTGSILDDIGSMFDDLADQLDAMLE DIP protein Q8WXD9 original sequence version: 76 CDF60E68B81E884A caskin1-1 5 11 unspecified role unspecified role unspecified role unspecified role SAM domain Region 470-615 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction caskin1-2 7 11 unspecified role unspecified role unspecified role unspecified role SAM domain Region 470-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction caskin1-caskin1-1 4 11 unspecified role unspecified role unspecified role unspecified role SAM domain Region 470-605 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Gly566Lys G566K mutation mutation certain certain sequence position certain certain certain sequence position certain G K This feature has been corrected as a result of our quality control procedures. The original label was 'G566K'. Wed May 02 16:38:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly566lys' Tue Jun 30 17:20:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly566Lys' ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role SAM domain Region 470-605 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Gly520Glu G520E mutation mutation certain certain sequence position certain certain certain sequence position certain G E This feature has been corrected as a result of our quality control procedures. The original label was 'G520E'. Wed May 02 17:51:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly520glu' Tue Jun 30 18:46:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly520Glu' ecolx Escherichia coli direct interaction direct interaction caskin1-3 6 11 unspecified role unspecified role unspecified role unspecified role Q8WXD9:p.Gly520Glu G520E mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G E This feature has been corrected as a result of our quality control procedures. The original label was 'G520E'. Wed May 02 20:18:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly520glu' Tue Jun 30 17:42:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly520Glu' Q8WXD9:p.Gly566Lys G566K mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G K This feature has been corrected as a result of our quality control procedures. The original label was 'G566K'. Wed May 02 16:38:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly566lys' Tue Jun 30 17:57:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly566Lys' CID-SAM domain Region 375-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction caskin1-4 2 11 unspecified role unspecified role unspecified role unspecified role SAM domain Region 470-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction caskin1-caskin1-2 3 11 unspecified role unspecified role prey prey SAM domain Region 470-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Gly566Lys G566K mutation mutation certain certain sequence position certain certain certain sequence position certain G K This feature has been corrected as a result of our quality control procedures. The original label was 'G566K'. Wed May 02 15:18:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly566lys' Tue Jun 30 19:35:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly566Lys' ecolx Escherichia coli 11 unspecified role unspecified role bait bait SAM domain Region 470-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Gly520Glu G520E mutation mutation certain certain sequence position certain certain certain sequence position certain G E This feature has been corrected as a result of our quality control procedures. The original label was 'G520E'. Wed May 02 18:38:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly520glu' Tue Jun 30 19:22:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly520Glu' ecolx Escherichia coli direct interaction direct interaction cask-caskin1-5 9 10 unspecified role unspecified role unspecified role unspecified role Region 1-310 Region 1-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role CID-SAM domain Region 375-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Gly520Glu G520E mutation mutation certain certain sequence position certain certain certain sequence position certain G E This feature has been corrected as a result of our quality control procedures. The original label was 'G520E'. Wed May 02 15:52:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly520glu' Tue Jun 30 19:53:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly520Glu' Q8WXD9:p.Gly566Lys G566K mutation mutation certain certain sequence position certain certain certain sequence position certain G K This feature has been corrected as a result of our quality control procedures. The original label was 'G566K'. Wed May 02 17:36:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly566lys' Tue Jun 30 17:29:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly566Lys' ecolx Escherichia coli direct interaction direct interaction caskin1-5 8 11 unspecified role unspecified role unspecified role unspecified role SAM domain Region 470-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cask-caskin1-6 1 10 unspecified role unspecified role unspecified role unspecified role Region 1-310 Region 1-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role CID-SAM domain Region 375-605 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction