DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253542X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253544X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253543X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253545X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Goloudina AR., Tanoue K., Hammann A., Fourmaux E., Le Guezennec X., Bulavin DV., Mazur SJ., Appella E., Garrido C., Demidov ON. DIP original experiment: DIP-253541X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Wip1 promotes RUNX2-dependent apoptosis in p53-negative tumors and protects normal tissues during treatment with anticancer agents. ppm1d_human Protein phosphatase 1D Protein phosphatase 2C isoform delta p53-induced protein phosphatase 1 Protein phosphatase magnesium-dependent 1 delta PPM1D WIP1 protein protein human Homo sapiens Human MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPGGEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAKALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRKTVCVC DIP protein O15297 original sequence version: 119 E6C5884CF04008B7 atm1981ps ATM peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) AFEEGSQSTTIGY runx2_human Runt-related transcription factor 2 Oncogene AML-3 Polyomavirus enhancer-binding protein 2 alpha A subunit SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit Osteoblast-specific transcription factor 2 Core-binding factor subunit alpha-1 Acute myeloid leukemia 3 protein RUNX2 AML3 CBFA1 OSF2 PEBP2A protein protein human Homo sapiens Human MASNSLFSTVTPCQQNFFWDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSTLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSGSYQFPMVPGGDRSPSRMLPPCTTTSNGSTLLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY DIP protein Q13950 original sequence version: 155 DIP protein Q13950 original sequence version: 138 DIP protein Q13950 original sequence version: 108 DIP protein Q13950 original sequence version: 157 DIP protein Q13950 original sequence version: 135 DIP protein Q13950-1 original sequence version: 135 44C4F3867D6F3EB1 ppm1d-runx2-1 5 8 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association atm1981ps-ppm1d-1 2 7 enzyme target enzyme target substrate substrate unspecified role unspecified role pS6 pS6 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme unspecified role unspecified role Catalytic domain Region 1-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation runx2-ppm1d-1 1 8 unspecified role unspecified role bait bait human Homo sapiens Human 6 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association ppm1d-runx2-2 4 8 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 425-437 Region 425-437 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS432 pS432 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme unspecified role unspecified role Catalytic domain Region 1-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation runx2-ppm1d-2 3 8 unspecified role unspecified role bait bait human Homo sapiens Human 6 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association