IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B. Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B. Nature 2011 imex curation Vivian JP., Duncan RC., Berry R., O'Connor GM., Reid HH., Beddoe T., Gras S., Saunders PM., Olshina MA., Widjaja JM., Harpur CM., Lin J., Maloveste SM., Price DA., Lafont BA., McVicar DW., Clements CS., Brooks AG., Rossjohn J. Originally I did not enter data from Fig 3B nor 4F ("Transfection Studies" in the Methods section, I assume) as no images of FACs results are shown, and no details of Participant Detection for B-57 tetramers are stated. There must be some fluorescent tag on B57, or a fluorescently labelled anti-B57 antibody?? - but no mention of this, and if not, I cannot see how binding is detected. However on looking at other work by this group I decided to enter the data-with an exp-mod. agbrooks@unimelb.edu.au,jamie.rossjohn@monash.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Vivian JP., Duncan RC., Berry R., O'Connor GM., Reid HH., Beddoe T., Gras S., Saunders PM., Olshina MA., Widjaja JM., Harpur CM., Lin J., Maloveste SM., Price DA., Lafont BA., McVicar DW., Clements CS., Brooks AG., Rossjohn J. Originally I did not enter data from Fig 3B nor 4F ("Transfection Studies" in the Methods section, I assume) as no images of FACs results are shown, and no details of Participant Detection for B-57 tetramers are stated. There must be some fluorescent tag on B57, or a fluorescently labelled anti-B57 antibody?? - but no mention of this, and if not, I cannot see how binding is detected. However on looking at other work by this group I decided to enter the data-with an exp-mod. agbrooks@unimelb.edu.au,jamie.rossjohn@monash.edu Accepted 2017-MAR-24 AT 12:19 GMT AT 12:19 GMT by ORCHARD Nature 2011 imex curation Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B. Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B. Nature 2011 imex curation Vivian JP., Duncan RC., Berry R., O'Connor GM., Reid HH., Beddoe T., Gras S., Saunders PM., Olshina MA., Widjaja JM., Harpur CM., Lin J., Maloveste SM., Price DA., Lafont BA., McVicar DW., Clements CS., Brooks AG., Rossjohn J. Originally I did not enter data from Fig 3B nor 4F ("Transfection Studies" in the Methods section, I assume) as no images of FACs results are shown, and no details of Participant Detection for B-57 tetramers are stated. There must be some fluorescent tag on B57, or a fluorescently labelled anti-B57 antibody?? - but no mention of this, and if not, I cannot see how binding is detected. However on looking at other work by this group I decided to enter the data-with an exp-mod. agbrooks@unimelb.edu.au,jamie.rossjohn@monash.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Vivian JP., Duncan RC., Berry R., O'Connor GM., Reid HH., Beddoe T., Gras S., Saunders PM., Olshina MA., Widjaja JM., Harpur CM., Lin J., Maloveste SM., Price DA., Lafont BA., McVicar DW., Clements CS., Brooks AG., Rossjohn J. Supplementary Figure 1 peptide sequences taken from pmid:25740999 Originally I did not enter data from Fig 3B nor 4F ("Transfection Studies" in the Methods section, I assume) as no images of FACs results are shown, and no details of Participant Detection for B-57 tetramers are stated. There must be some fluorescent tag on B57, or a fluorescently labelled anti-B57 antibody?? - but no mention of this, and if not, I cannot see how binding is detected. However on looking at other work by this group I decided to enter the data-with an exp-mod. agbrooks@unimelb.edu.au,jamie.rossjohn@monash.edu Accepted 2017-MAR-24 AT 12:19 GMT AT 12:19 GMT by ORCHARD Nature 2011 imex curation Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B. Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen B. Nature 2011 imex curation Vivian JP., Duncan RC., Berry R., O'Connor GM., Reid HH., Beddoe T., Gras S., Saunders PM., Olshina MA., Widjaja JM., Harpur CM., Lin J., Maloveste SM., Price DA., Lafont BA., McVicar DW., Clements CS., Brooks AG., Rossjohn J. Originally I did not enter data from Fig 3B nor 4F ("Transfection Studies" in the Methods section, I assume) as no images of FACs results are shown, and no details of Participant Detection for B-57 tetramers are stated. There must be some fluorescent tag on B57, or a fluorescently labelled anti-B57 antibody?? - but no mention of this, and if not, I cannot see how binding is detected. However on looking at other work by this group I decided to enter the data-with an exp-mod. agbrooks@unimelb.edu.au,jamie.rossjohn@monash.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs tag fluorescence tag visualisation by fluorescence tag fluorescence Vivian JP., Duncan RC., Berry R., O'Connor GM., Reid HH., Beddoe T., Gras S., Saunders PM., Olshina MA., Widjaja JM., Harpur CM., Lin J., Maloveste SM., Price DA., Lafont BA., McVicar DW., Clements CS., Brooks AG., Rossjohn J. Originally I did not enter data from Fig 3B nor 4F ("Transfection Studies" in the Methods section, I assume) as no images of FACs results are shown, and no details of Participant Detection for B-57 tetramers are stated. There must be some fluorescent tag on B57, or a fluorescently labelled anti-B57 antibody?? - but no mention of this, and if not, I cannot see how binding is detected. However on looking at other work by this group I decided to enter the data-with an exp-mod. "Cells were analysed on a flow cytometer after washing". Previous and later work by the same group/authors have been used to provide some experimental details. e.g. pmid:25740999 et al. Biotinylated pMHCI monomers were conjugated by addition of fluorochrome (phycoerythrin) -conjugated streptavidin to produce tetrameric complexes. agbrooks@unimelb.edu.au,jamie.rossjohn@monash.edu Accepted 2017-MAR-24 AT 12:19 GMT AT 12:19 GMT by ORCHARD Nature 2011 imex curation 1b57_human HLA class I histocompatibility antigen, B-57 alpha chain HLA-B Bw-57 HLAB MHC class I antigen B*57 protein protein human Homo sapiens Human MRVTAPRTVLLLLWGAVALTETWAGSHSMRYFYTAMSRPGRGEPRFIAVGYVDDTQFVRFDSDAASPRMAPRAPWIEQEGPEYWDGETRNMKASAQTYRENLRIALRYYNQSEAGSHIIQVMYGCDVGPDGRLLRGHDQSAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAARVAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA The protein could not be merged with EBI-1046513 because of some conflicts with the protein sequence (features which cannot be shifted). C1EFCF089096AFF9 igkc_human Immunoglobulin kappa constant IGKC Ig kappa chain C region Ig kappa chain C region AG Ig kappa chain C region CUM Ig kappa chain C region EU Ig kappa chain C region OU Ig kappa chain C region ROY Ig kappa chain C region TI protein protein human Homo sapiens Human RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC B97BEEBBA4D0B291 p61769-pro_0000018779 Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM 21 119 B6EE395752B3322D ki3l1_human Killer cell immunoglobulin-like receptor 3DL1 KIR3DL1 CD158E NKAT3 NKB1 CD158 antigen-like family member E HLA-BW4-specific inhibitory NK cell receptor MHC class I NK cell receptor Natural killer-associated transcript 3 p70 natural killer cell receptor clones CL-2/CL-11 protein protein human Homo sapiens Human MSLMVVSMACVGLFLVQRAGPHMGGQDKPFLSAWPSAVVPRGGHVTLRCHYRHRFNNFMLYKEDRIHIPIFHGRIFQESFNMSPVTTAHAGNYTCRGSHPHSPTGWSAPSNPVVIMVTGNHRKPSLLAHPGPLVKSGERVILQCWSDIMFEHFFLHKEGISKDPSRLVGQIHDGVSKANFSIGPMMLALAGTYRCYGSVTHTPYQLSAPSDPLDIVVTGPYEKPSLSAQPGPKVQAGESVTLSCSSRSSYDMYHLSREGGAHERRLPAVRKVNRTFQADFPLGPATHGGTYRCFGSFRHSPYEWSDPSDPLLVSVTGNPSSSWPSPTEPSSKSGNPRHLHILIGTSVVIILFILLLFFLLHLWCSNKKNAAVMDQEPAGNRTANSEDSDEQDPEEVTYAQLDHCVFTQRKITRPSQRPKTPPTDTILYTELPNAKPRSKVVSCP 47DEA12BBAFDEC53 hla_b-kir3dl1-4 2 4 unspecified role unspecified role bait bait p.Arg107Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:03:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg107ala' extracellular_region sufficient to bind sufficient binding region sufficient to bind range range range range p.Ile104Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A Tue May 09 10:39:18 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ile80ala' Wed May 02 14:31:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104ala' p.Gln96Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 15:04:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln96ala' p.Glu100Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Tue May 09 10:22:28 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln100ala' Wed May 02 15:35:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu100ala' p.Arg103Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:27:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg103ala' p.Ile104Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 17:22:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile104thr' p.Glu113Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:58:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu113ala' p.Ile166Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A Wed May 02 17:52:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile166ala' p.Arg169Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:11:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg169ala' p.Lys170Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:31:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys170ala' p.Ala173Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A G Wed May 02 15:22:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala173gly' p.Arg175Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:46:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175ala' p.Gly40[4] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G GGGG Tue May 09 10:42:13 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gly40-40gly_gly_gly_gly' Wed May 02 19:19:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly40gly_gly_gly_gly' arg_pro_gly_arg_gly_glu38-43del_del_del_gly_gly_gly binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RPGRGE ...GGG ecoli Escherichia coli (strain K12) 6 ancillary ancillary unspecified role unspecified role whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain possible_his_and_secretion_tags tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 ancillary ancillary unspecified role unspecified role fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P01834:p.Ser101Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S R Tue May 09 10:23:05 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser100arg' Wed May 02 17:06:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser101arg' Tue Jun 30 19:26:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser101Arg' P01834:p.Ser101Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue May 09 10:30:10 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser100ala' Wed May 02 15:08:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser101ala' Tue Jun 30 17:34:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser101Ala' P01834:p.Ser101Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S F Tue May 09 10:43:14 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser100phe' Wed May 02 15:09:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser101phe' Tue Jun 30 17:07:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser101Phe' P01834:p.Ser101Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S E Tue May 09 10:47:13 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser100glu' Wed May 02 15:12:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser101glu' Tue Jun 30 19:29:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser101Glu' P01834:p.Ser101His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S H Tue May 09 10:43:48 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser100his' Wed May 02 19:40:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser101his' Tue Jun 30 17:23:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser101His' P01834:p.Ser101Leu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S L Tue May 09 10:40:26 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser100leu' Wed May 02 18:40:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser101leu' Tue Jun 30 19:51:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser101Leu' 31 11 26 physical association physical association Figure 3A, Supp fig 1 A/B, Suplementary Table 1. B5701 allele I have entered the "long" mutation as the instructions suggest as a "binding region".... hla_b-kir3dl1-3 1 4 unspecified role unspecified role neutral component neutral component extracellular_region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component unknown_secretion_tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range extracellular_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293s Human 293S cell line Human 293s 293S cell line 5 unspecified role unspecified role neutral component neutral component fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 40 42 48 physical association physical association Supplementary Table 2 B5701 allele I have added (GSGSDDDDKGS) the "expression tag" sequence to the feature - a google search comes up with nowt. 1.8A R work =0.211 R free =0.237 b2m-hla_b-4 3 4 unspecified role unspecified role neutral component neutral component biotin/avidin/phycoerythrin_tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined extracellular_region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component P43629:p.Phe30Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:29:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe30ala' Tue Jun 30 19:45:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe30Ala' P43629:p.Trp34Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain W A Wed May 02 19:55:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp34ala' Tue Jun 30 18:42:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp34Ala' P43629:p.Leu187_Ala188delinsProVal mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LA PV Tue May 09 10:52:20 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'leu187pro,ala188val' Wed May 02 17:52:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_ala187-188pro_val' Tue Jun 30 18:32:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu187_Ala188delinsProVal' P43629:p.Met186Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M T Wed May 02 17:12:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met186thr' Tue Jun 30 19:11:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met186Thr' P43629:p.Met186_Leu187delinsThrPro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain ML TP Tue May 09 10:38:00 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'met186thr,leu187pro' Wed May 02 16:26:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met_leu186-187thr_pro' Tue Jun 30 17:57:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met186_Leu187delinsThrPro' P43629:p.Ser32Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:08:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser32ala' Tue Jun 30 17:09:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser32Ala' P43629:p.His53Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Wed May 02 19:15:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his53ala' Tue Jun 30 18:52:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His53Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P43629:p.His50Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Wed May 02 19:30:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his50ala' Tue Jun 30 16:17:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His50Ala' P43629:p.Phe55Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:38:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe55ala' Tue Jun 30 19:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe55Ala' P43629:p.Lys157Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:14:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys157ala' Tue Jun 30 15:43:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys157Ala' P43629:p.Gly159Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 18:51:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly159ala' Tue Jun 30 17:49:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly159Ala' P43629:p.Ser161Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:06:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser161ala' Tue Jun 30 18:34:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser161Ala' P43629:p.Met186Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Wed May 02 20:24:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met186ala' Tue Jun 30 16:16:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met186Ala' P43629:p.Leu187Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L V Wed May 02 19:56:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu187val' Tue Jun 30 16:41:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu187Val' P43629:p.Leu187Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:21:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu187ala' Tue Jun 30 18:36:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu187Ala' P43629:p.Ala188Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A G Wed May 02 18:11:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala188gly' Tue Jun 30 17:59:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala188Gly' P43629:p.Pro220Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain P A Wed May 02 14:49:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro220ala' Tue Jun 30 16:51:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro220Ala' P43629:p.Tyr221Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 14:32:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr221phe' Tue Jun 30 17:36:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr221Phe' P43629:p.Tyr221Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:21:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr221ala' Tue Jun 30 19:37:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr221Ala' P43629:p.Glu222Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:31:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu222ala' Tue Jun 30 18:46:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu222Ala' P43629:p.Ser248Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:05:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser248ala' Tue Jun 30 15:41:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser248Ala' P43629:p.Ser249Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:40:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser249ala' Tue Jun 30 20:16:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser249Ala' P43629:p.Asp251Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:11:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp251ala' Tue Jun 30 17:17:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp251Ala' P43629:p.Phe297Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:52:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe297ala' Tue Jun 30 20:05:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe297Ala' P43629:p.Arg298Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:03:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg298ala' Tue Jun 30 16:52:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg298Ala' P43629:p.His299Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain H A Wed May 02 14:26:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his299ala' Tue Jun 30 18:20:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His299Ala' P43629:p.Ser300Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:41:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser300ala' Tue Jun 30 20:08:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser300Ala' P43629:p.Glu303Gln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 14:29:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu303gln' Tue Jun 30 18:32:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu303Gln' P43629:p.Glu303Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 20:19:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu303ala' Tue Jun 30 18:28:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu303Ala' P43629:p.Gly159Trp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G W Wed May 02 17:43:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly159trp' Tue Jun 30 17:58:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly159Trp' P43629:p.Leu187Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L R Wed May 02 17:10:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu187arg' Tue Jun 30 17:36:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu187Arg' P43629:p.Pro220Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P L Wed May 02 17:06:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro220leu' Tue Jun 30 19:04:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro220Leu' P43629:p.Leu187Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L P Wed May 02 20:25:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu187pro' Tue Jun 30 15:52:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu187Pro' P43629:p.Ala188Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V Wed May 02 15:08:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala188val' Tue Jun 30 15:48:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala188Val' P43629:p.His299Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain H A Wed May 02 17:05:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his299ala' Tue Jun 30 18:00:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His299Ala' P43629:p.Ser300Leu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S L Wed May 02 16:42:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser300leu' Tue Jun 30 18:49:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser300Leu' P43629:p.Glu303Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E V Wed May 02 20:09:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu303val' Tue Jun 30 18:43:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu303Val' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 5 unspecified role unspecified role neutral component neutral component fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 54 52 95 physical association physical association Figure 3B, 4F B5701 allele no idea what the stoichiometry of beta-2 microglobulin and peptide w.r.t .B57 MHC1 molecule (tetramer). Peptide:I have assumed the same WT one as used before-all the other publications use one as the complex is unstable without the peptide - but nowt mentioned here.. I found ONE mutation mentioned in both Figs 3B and 4F - but may have missed others?