DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure and mechanism of the chromatin remodelling factor ISW1a. Structure and mechanism of the chromatin remodelling factor ISW1a. Nature 2011 imex curation Yamada K., Frouws TD., Angst B., Fitzgerald DJ., DeLuca C., Schimmele K., Sargent DF., Richmond TJ. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Yamada K., Frouws TD., Angst B., Fitzgerald DJ., DeLuca C., Schimmele K., Sargent DF., Richmond TJ. DIP original experiment: DIP-219076X Nature 2011 IMEx Structure and mechanism of the chromatin remodelling factor ISW1a. Structure and mechanism of the chromatin remodelling factor ISW1a. Structure and mechanism of the chromatin remodelling factor ISW1a. Nature 2011 imex curation Yamada K., Frouws TD., Angst B., Fitzgerald DJ., DeLuca C., Schimmele K., Sargent DF., Richmond TJ. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yamada K., Frouws TD., Angst B., Fitzgerald DJ., DeLuca C., Schimmele K., Sargent DF., Richmond TJ. DIP original experiment: DIP-219078X Nature 2011 IMEx Structure and mechanism of the chromatin remodelling factor ISW1a. Structure and mechanism of the chromatin remodelling factor ISW1a. Structure and mechanism of the chromatin remodelling factor ISW1a. Nature 2011 imex curation Yamada K., Frouws TD., Angst B., Fitzgerald DJ., DeLuca C., Schimmele K., Sargent DF., Richmond TJ. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yamada K., Frouws TD., Angst B., Fitzgerald DJ., DeLuca C., Schimmele K., Sargent DF., Richmond TJ. DIP original experiment: DIP-219077X Nature 2011 IMEx Structure and mechanism of the chromatin remodelling factor ISW1a. isw1_yeast ISWI chromatin-remodeling complex ATPase ISW1 YBR1633 YBR245C ISW1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAYMLAIANFHFFKFYTRMRKKHENNSCNEKDKDENLFKIILAIFLQEKKKYDCISSGSIMTASEEYLENLKPFQVGLPPHDPESNKKRYLLKDANGKKFDLEGTTKRFEHLLSLSGLFKHFIESKAAKDPKFRQVLDVLEENKANGKGKGKHQDVRRRKTEHEEDAELLKEEDSDDDESIEFQFRESPAYVNGQLRPYQIQGVNWLVSLHKNKIAGILADEMGLGKTLQTISFLGYLRYIEKIPGPFLVIAPKSTLNNWLREINRWTPDVNAFILQGDKEERAELIQKKLLGCDFDVVIASYEIIIREKSPLKKINWEYIIIDEAHRIKNEESMLSQVLREFTSRNRLLITGTPLQNNLHELWALLNFLLPDIFSDAQDFDDWFSSESTEEDQDKIVKQLHTVLQPFLLRRIKSDVETSLLPKKELNLYVGMSSMQKKWYKKILEKDLDAVNGSNGSKESKTRLLNIMMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNAAKLQVLDKLLKKLKEEGSRVLIFSQMSRLLDILEDYCYFRNYEYCRIDGSTAHEDRIQAIDDYNAPDSKKFVFLLTTRAGGLGINLTSADVVVLYDSDWNPQADLQAMDRAHRIGQKKQVKVFRLVTDNSVEEKILERATQKLRLDQLVIQQNRTSLKKKENKADSKDALLSMIQHGAADVFKSGTSTGSAGTPEPGSGEKGDDIDLDELLLKSENKTKSLNAKYETLGLDDLQKFNQDSAYEWNGQDFKKKIQRDIISPLLLNPTKRERKENYSIDNYYKDVLNTGRSSTPSHPRMPKPHVFHSHQLQPPQLKVLYEKERMWTAKKTGYVPTMDDVKAAYGDISDEEEKKQKLELLKLSVNNSQPLTEEEEKMKADWESEGFTNWNKLEFRKFITVSGKYGRNSIQAIARELAPGKTLEEVRAYAKAFWSNIERIEDYEKYLKIIENEEEKIKRVKMQQEALRRKLSEYKNPFFDLKLKHPPSSNNKRTYSEEEDRFILLMLFKYGLDRDDVYELVRDEIRDCPLFELDFYFRSRTPVELARRGNTLLQCLEKEFNAGIVLDDATKDRMKKEDENGKRIREEFADQTANEKENVDGVESKKAKIEDTSNVGTEQLVAEKIPENETTH 2CF86BAF553E6695 2CF86BAF553E6695 ioc3_yeast ISWI one complex protein 3 YFR013W IOC3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSPSNSIQNLQQEAQGSSSAQLADHDHDRVSMAMPLQTDQSVSVSQSSDNLRRSRRVPKPRTSIYDEYEEELKERANKPKRKRPAPPKKKAPSTQNSKSNDKVEKKKTTSIAKDGKPTLKTNDKKVAPKPKPAHEQVEPALIPSNWTSVIPLLTSDFKNQYSVISRLKNPNMKPVPYAGDIIKLMAFINKFSSFFHSDLQNLSFQDFEVGLDLYPGDPNGSAAGIVKGPEDTSLLLYPDFMAIKDIVYCQDKMNLLFLSLLDLTFTENFDGKSAKKKGPLTTWENLKSSSKKVFSNPLYRLRLVAREWGYPREWRQQLPSDQDISKPKTALFEQDEQTPVVDPSHPEILTPNIYTWNANEPLPLESNPLYNREMDKNGILALKPMDRVVLLRALTDWCASHSSAIHDEIYKLTHGKKDPVFGIQTQQVPRYTIEGVDNTINQFKKLCSLIQSRYEIRSKKKHFVKQLKEGKKPDLSRKLEILKEIKAELKNAVKSEKDELLFSLYDKWVPLFEGELPDQPLANPFSERLYKLRLQEFFLGRVPHIGDFYMPRLHSYGDSLEMSTFTDLRNLQALLSKFKNNEYNAFTLFENDGQSMSAQFKLFYHDTPSLAHDVARGRNTSGKVYWYELCHDSATLLEFLEFLDYKIVKPQDEKKEGNEKEKEALNNEAHILEQKSTTDNNPSINTNPLPKDAKYNTARKKLQILKEFLSDYYFILRQFEQMKVQFADMKPGKRQLRRIQRQTVNYNTEYDSEEYVDDEEDDEADIYDDNDNDSSFDDGRVKRQRT 1919A8A1F58B5340 1919A8A1F58B5340 hss-loc3-1 1 5 unspecified role unspecified role prey prey Region 127-749 Region 127-749 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role bait bait Region 763-1129 Region 763-1129 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association hss-loc3-2 2 4 unspecified role unspecified role unspecified role unspecified role Region 763-1129 Region 763-1129 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role unspecified role unspecified role Region 127-749 Region 127-749 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction hss-loc3-3 3 5 unspecified role unspecified role unspecified role unspecified role Region 127-749 Region 127-749 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role unspecified role unspecified role Region 763-1129 Region 763-1129 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction