DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219084X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219085X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219092X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219088X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219090X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219091X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219089X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219093X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219087X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219094X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Crystal structure of inhibitor of κB kinase β. Nature 2011 imex curation Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Xu G., Lo YC., Li Q., Napolitano G., Wu X., Jiang X., Dreano M., Karin M., Wu H. DIP original experiment: DIP-219086X Nature 2011 IMEx Crystal structure of inhibitor of κB kinase β. ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS DIP protein O14920 original sequence version: 89 DIP protein O14920 original sequence version: 100 DIP protein O14920 original sequence version: 121 DIP protein O14920 original sequence version: 126 DIP protein O14920 original sequence version: 136 DIP protein O14920 original sequence version: 128 DIP protein O14920-1 original sequence version: 151 DIP protein O14920 original sequence version: 142 DIP protein O14920 original sequence version: 159 DIP protein O14920 original sequence version: 93 DIP protein O14920 original sequence version: 124 DIP protein O14920-1 original sequence version: 178 DIP protein O14920 original sequence version: 85 F9CADF671AE9E14E q6int1_xenla I-kappa-B kinase ikbkb.L MGC80376 ikbkb protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MKERLGTGGFGYVLRWIHQDTGEQVAIKQCRQELSPKNRERWCLEIQIMKKLNHPNVVSAREVPDGLQKLAPNDLPLLAMEYCEGGDLRKYLNQFENCCGLKEGPIRTLLSDISSALRYLHENRIIHRDLKPENIVLQPGPQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQKKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHGKVREKSNEHIVVYDDLTGAVKFSSVLPTPNHLSGILAGKLERWLQCMLMWHQRQRGTDPQNPNVGCFQALDSILSLKLLSVMNMVSGRVHTYPVTENENLQNLKSWLQQDTGIPEEEQELLQASGLALNSAQPLTQYVIDCTVIDGRQGEGDLIFLFDNRKTVYEPQISLPAHPESVSIVLQDPKRPLTYTHLRRVWGQIWQTIRALKEDCARLLQGQRTSMVNLLRYNTELSKKKNSMTSECEQLKAKLDFFRSSIQIDLEKYSEQMEFGITSEKLLSAWREMEQAVELCGREREVQALVDKMMALQTDSVDLQRNPLGRKHRGTLDDLEEQARDLYRRLRERPRDQRTPGDSNDMVRLLILAIQSFEKRVILIYDQLSKTVVCKRKALELSPKVKEVMNLMREDEKIVVRRQEKRQQELWNLLKIACSKVRGPVSGSPDSLGTSRMPGPSQLLSQPPVLSVSLAQEYVKKSEDLRSESLNLCSQLESSMRSFMKEQDGSLMSLDWSWLLQQEEESGASPITTS DIP protein Q6INT1 original sequence version: 48 nemo_human NF-kappa-B essential modulator IKBKG FIP3 NEMO NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 FIP-3 protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein Q9Y6K9 original sequence version: 85 DIP protein Q9Y6K9 original sequence version: 118 DIP protein Q9Y6K9 original sequence version: 123 DIP protein Q9Y6K9 original sequence version: 141 DIP protein Q9Y6K9 original sequence version: 125 DIP protein Q9Y6K9 original sequence version: 156 DIP protein Q9Y6K9 original sequence version: 83 DIP protein Q9Y6K9 original sequence version: 132 DIP protein Q9Y6K9 original sequence version: 124 DIP protein Q9Y6K9 original sequence version: 90 322D1037881447FF ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 ikbkb-19 1 13 unspecified role unspecified role unspecified role unspecified role Region 4-675 Region 4-675 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction nfkbia-ikbkb-1 9 12 unspecified role unspecified role prey prey ULD-SDD domain Region 307-678 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role bait bait Region 54-317 Region 54-317 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ikbkb-nfkbia-19 7 12 enzyme enzyme unspecified role unspecified role KD-ULD domain Region 1-400 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ikbkb-20 2 13 unspecified role unspecified role unspecified role unspecified role Region 4-675 Region 4-675 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction ikbkb-21 6 12 unspecified role unspecified role unspecified role unspecified role KD-ULD-SDD domain Region 1-678 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O14920:p.[Leu654Asp;Trp655Asp] L654D/W655D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W D certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L654D/W655D'. Wed May 02 16:36:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu654asp,trp655asp' Tue Jun 30 16:00:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu654Asp;Trp655Asp]' O14920:p.[Trp655Asp;Leu658Asp] W655D/L658D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain W D This feature has been corrected as a result of our quality control procedures. The original label was 'W655D/L658D'. Wed May 02 18:37:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp655asp,leu658asp' Tue Jun 30 18:38:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp655Asp;Leu658Asp]' ecolx Escherichia coli direct interaction direct interaction ikbkb-nfkbia-20 8 12 enzyme enzyme unspecified role unspecified role Region 1-643 Region 1-643 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 15 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation nfkbia-ikbkb-2 11 12 unspecified role unspecified role prey prey ULD-SDD domain Region 307-678 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role bait bait Region 54-282 Region 54-282 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 282-317 Region 282-317 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ikbkg-ikbkb-13 10 14 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 12 unspecified role unspecified role prey prey O14920:p.[Leu654Asp;Trp655Asp] L654D/W655D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W D certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L654D/W655D'. Wed May 02 16:47:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu654asp,trp655asp' Tue Jun 30 18:13:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu654Asp;Trp655Asp]' O14920:p.[Trp655Asp;Leu658Asp] W655D/L658D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W D certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'W655D/L658D'. Wed May 02 17:48:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp655asp,leu658asp' Tue Jun 30 15:51:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp655Asp;Leu658Asp]' HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association ikbkb-nfkbia-21 4 12 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 15 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-54 Region 1-54 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P25963:p.[Ser32Ala;Ser36Ala] S32A/S36A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S32A/S36A'. Wed May 02 18:04:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser32ala,ser36ala' Tue Jun 30 18:27:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser32Ala;Ser36Ala]' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ikbkb-nfkbia-22 5 12 enzyme enzyme unspecified role unspecified role Region 1-643 Region 1-643 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 enzyme target enzyme target substrate substrate unspecified role unspecified role pS32/S36 pS32/S36 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ikbkb-22 3 12 unspecified role unspecified role unspecified role unspecified role O14920:p.[Leu654Asp;Trp655Asp] L654D/W655D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W D certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L654D/W655D'. Wed May 02 19:08:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu654asp,trp655asp' Tue Jun 30 19:51:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu654Asp;Trp655Asp]' O14920:p.[Trp655Asp;Leu658Asp] W655D/L658D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain W D This feature has been corrected as a result of our quality control procedures. The original label was 'W655D/L658D'. Wed May 02 18:16:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp655asp,leu658asp' Tue Jun 30 17:56:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp655Asp;Leu658Asp]' ecolx Escherichia coli direct interaction direct interaction