DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. DIP original experiment: DIP-249131X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. DIP original experiment: DIP-249127X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. DIP original experiment: DIP-249130X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. DIP original experiment: DIP-249129X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Quintero FJ., Martinez-Atienza J., Villalta I., Jiang X., Kim WY., Ali Z., Fujii H., Mendoza I., Yun DJ., Zhu JK., Pardo JM. DIP original experiment: DIP-249128X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. nhx7_arath Sodium/hydrogen exchanger 7 NHX7 SOS1 Na(+)/H(+) exchanger 7 Protein SALT OVERLY SENSITIVE 1 F14H20.5 At2g01980 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTTVIDATMAYRFLEEATDSSSSSSSSKLESSPVDAVLFVGMSLVLGIASRHLLRGTRVPYTVALLVIGIALGSLEYGAKHNLGKIGHGIRIWNEIDPELLLAVFLPALLFESSFSMEVHQIKRCLGQMVLLAVPGVLISTACLGSLVKVTFPYEWDWKTSLLLGGLLSATDPVAVVALLKELGASKKLSTIIEGESLMNDGTAIVVFQLFLKMAMGQNSDWSSIIKFLLKVALGAVGIGLAFGIASVIWLKFIFNDTVIEITLTIAVSYFAYYTAQEWAGASGVLTVMTLGMFYAAFARTAFKGDSQKSLHHFWEMVAYIANTLIFILSGVVIAEGILDSDKIAYQGNSWRFLFLLYVYIQLSRVVVVGVLYPLLCRFGYGLDWKESIILVWSGLRGAVALALSLSVKQSSGNSHISKETGTLFLFFTGGIVFLTLIVNGSTTQFVLRLLRMDILPAPKKRILEYTKYEMLNKALRAFQDLGDDEELGPADWPTVESYISSLKGSEGELVHHPHNGSKIGSLDPKSLKDIRMRFLNGVQATYWEMLDEGRISEVTANILMQSVDEALDQVSTTLCDWRGLKPHVNFPNYYNFLHSKVVPRKLVTYFAVERLESACYISAAFLRAHTIARQQLYDFLGESNIGSIVINESEKEGEEAKKFLEKVRSSFPQVLRVVKTKQVTYSVLNHLLGYIENLEKVGLLEEKEIAHLHDAVQTGLKKLLRNPPIVKLPKLSDMITSHPLSVALPPAFCEPLKHSKKEPMKLRGVTLYKEGSKPTGVWLIFDGIVKWKSKILSNNHSLHPTFSHGSTLGLYEVLTGKPYLCDLITDSMVLCFFIDSEKILSLQSDSTIDDFLWQESALVLLKLLRPQIFESVAMQELRALVSTESSKLTTYVTGESIEIDCNSIGLLLEGFVKPVGIKEELISSPAALSPSNGNQSFHNSSEASGIMRVSFSQQATQYIVETRARAIIFNIGAFGADRTLHRRPSSLTPPRSSSSDQLQRSFRKEHRGLMSWPENIYAKQQQEINKTTLSLSERAMQLSIFGSMVNVYRRSVSFGGIYNNKLQDNLLYKKLPLNPAQGLVSAKSESSIVTKKQLETRKHACQLPLKGESSTRQNTMVESSDEEDEDEGIVVRIDSPSKIVFRNDL DIP protein Q9LKW9 original sequence version: 102 DIP protein Q9LKW9 original sequence version: 80 DAEA315426F8AE7A cipko_arath CBL-interacting serine/threonine-protein kinase 24 SNF1-related kinase 3.11 Protein SALT OVERLY SENSITIVE 2 CIPK24 SnRK3.11 SOS2 At5g35410 F6I13.1 K21B8.3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKMRRVGKYEVGRTIGEGTFAKVKFARNTDTGDNVAIKIMAKSTILKNRMVDQIKREISIMKIVRHPNIVRLYEVLASPSKIYIVLEFVTGGELFDRIVHKGRLEESESRKYFQQLVDAVAHCHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVELLRTTCGTPNYVAPEVLSGQGYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINAAEFSCPPWFSAEVKFLIHRILDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDIRAVFDGIEGSYVAENVERNDEGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFKSHTRNFKTRLEGLSSIKAGQLAVVIEIYEVAPSLFMVDVRKAAGETLEYHKFYKKLCSKLENIIWRATEGIPKSEILRTITF DIP protein Q9LDI3 original sequence version: 90 99FDF3A778E1093D cnbl4_arath Calcineurin B-like protein 4 Protein SALT OVERLY SENSITIVE 3 CBL4 SOS3 At5g24270 MOP9.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS DIP protein O81223 original sequence version: 98 DF06C3973748AFF7 cipk24-cbl4-2 2 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction cipk24-nhx7-1 1 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1072-1146 Region 1072-1146 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 enzyme enzyme unspecified role unspecified role Region 1-307 Region 1-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9LDI3:p.Thr168Asp T168D mutation mutation certain certain sequence position certain certain certain sequence position certain T D This feature has been corrected as a result of our quality control procedures. The original label was 'T168D'. Wed May 02 18:23:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr168asp' Tue Jun 30 16:26:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr168Asp' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation cipk24-nhx7-2 5 6 unspecified role unspecified role prey prey Region 998-1145 Region 998-1145 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction cipk24-nhx7-3 3 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 441-1146 Region 441-1146 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9LKW9:p.[Ser1136Ala;Ser1138Ala] S1136A/S1138A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S1136A/S1138A'. Wed May 02 16:46:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1136ala,ser1138ala' Tue Jun 30 15:56:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1136Ala;Ser1138Ala]' Q9LKW9:p.Ser1136Ala S1136A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S1136A'. Wed May 02 16:28:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1136ala' Tue Jun 30 15:48:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser1136Ala' Q9LKW9:p.Ser1138Ala S1138A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S1138A'. Wed May 02 15:48:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1138ala' Tue Jun 30 17:37:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser1138Ala' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 enzyme enzyme unspecified role unspecified role Region 1-307 Region 1-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9LDI3:p.Thr168Asp T168D mutation mutation certain certain sequence position certain certain certain sequence position certain T D This feature has been corrected as a result of our quality control procedures. The original label was 'T168D'. Wed May 02 17:44:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr168asp' Tue Jun 30 18:05:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr168Asp' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation cipk24-nhx7-4 4 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 998-1146 Region 998-1146 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS1138 pS1138 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 enzyme enzyme unspecified role unspecified role Region 1-307 Region 1-307 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9LDI3:p.Thr168Asp T168D mutation mutation certain certain sequence position certain certain certain sequence position certain T D This feature has been corrected as a result of our quality control procedures. The original label was 'T168D'. Wed May 02 16:27:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr168asp' Tue Jun 30 17:53:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr168Asp' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation