DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253419X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253411X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253418X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253412X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253416X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253414X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253413X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253417X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. Only protein-protein interactions in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined James TW., Frias-Staheli N., Bacik JP., Levingston Macleod JM., Khajehpour M., García-Sastre A., Mark BL. DIP original experiment: DIP-253415X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. l_cchfi RNA-directed RNA polymerase L L Large structural protein Replicase Transcriptase protein protein cchfi Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) MDFLRSLDWTQVIAGQYVSNPRFNISDYFEIVRQPGDGNCFYHSIAELTMPNKTDHSYHYIKRLTESAARKYYQEEPEARLVGLSLEDYLKRMLSDNEWGSTLEASMLAKEMGITIIIWTVAASDEVEAGIKFGDGDVFTAVNLLHSGQTHFDALRILPQFETDTREALSLMDRVIAVDQLTSSSSDELQDYEDLALALTSAEESNRRSSLDEVTLSKKQAEILRQKASQLSKLVNKSQNIPTRVGRVLDCMFNCKLCVEISADTLILRPESKEKIGEIMSLRQLGHKLLTRDKQIKQEFSRMKLYVTKDLLDHLDVGGLLRAAFPGTGIERHMQLLHSEMILDICTVSLGVMLSTFLYGSNNKNKKKFITNCLLSTALSGKKVYKVLGNLGNELLYKAPRKALATVCSALFGKQINKLQNCFRTISPVSLLALRNLDFDCLSVQDYNGMIENMSKLDNTDVEFNHREIADLNQLTSRLITLRKEKDTDLLKQWFPESDLTRRSIRNAANAEEFVISEFFKKKDIMKFISTSGRAMSAGKIGNVLSYAHNLYLSKSSLNMTSEDISQLLIEIKRLYALQEDSEVEPIAIICDGIESNMKQLFAILPPDCARECEVLFDDIRNSPTHSTAWKHALRLKGTAYEGLFANCYGWQYIPEDIKPSLTMLIQTLFPDKFEDFLDRTQLHPEFRDLTPDFSLTQKVHFKRNQIPSVENVQISIDATLPESVEAVPVTERKMFPLPETPLSEVHSIERIMENFTRLMHGGRLSTKKRDGDPAEQGNQQSITEHESSSISAFKDYGERGIVEENHMKFSGEDQLETRQLLLVEVGFQTDIDGKIRTDHKKWKDILKLLELLGIKCSFIACADCSSTPPDRWWITEDRVRVLKNSVSFLFNKLSRNSPTEVTDIVVGAISTQKVRSYLKAGTATKTPVSTKDVLETWEKMKEHILNRPTGLTLPTSLEQAMRKGLVEGVVISKEGSESCINMLKENLDRITDEFERTKFKHELTQNITTSEKMLLSWLSEDIKSSRCGECLSNIKKAVDETANLSGKIELLAYNLQLTNHCSNCHPNGVNISNTSNVCKRCPKIEVVSHCENKGFEDSNECLTDLDRLVRLTLPGKTEKERRVKRNVEYLIKLMMSMSGIDCIKYPTGQLITHGRVSAKHNDGNLKDRSDDDQRLAEKIDTVRKELSESKLKDYSTYARGVISNSLKNLSRQGKSKCSVPRSWLEKVLFDLKVPTKDEEVLINIRNSLKARSEFVRNNDKLLIRSKEELKKCFDVQSFKLKKNKQPVPFQVDCILFKEVAAECMKRYIGTPYEGIVDTLVSLINVLTRFTWFQEVVLYGKICETFLRCCTEFNRSGVKLVKIRHCNINLSVKLPSNKKENMLCCLYSGNMELLQGPFYLNRRQAVLGSSYLYIVITLYIQVLQQYRCLEVINSVSEKTLQDIENHSMTLLEDSFREITFALEGRFEESYKIRTSRCRASGNFLNRSSRDHFISVVSGLNLVYGFLIKDNLLANSQQQNKQLQMLRFGMLAGLSRLVCPNELGKKFSTSCRRIEDNIARLYLQTSIYCSVRDVEDNVKHWKQRDLCPEVTIPCFTVYGTFVNSDRQLIFDIYNVHIYNKEMDNFDEGCISVLEETAERHMLWELDLMNSLCSDEKKDTRPARLLLGCPNVRKAATREGKKLLKLNSDTSTDTQSIASEVSDRRSYSSSKSRIRSIFGRYNSQKKPFELRSGLEVFNDPFNDYQQAITDICQFSEYTPNKESILKDCLQIIRKNPSHTMGSFELIQAISEFGMSKFPPENIDKARRDPKNWVSISEVTETTSIVASPRTHMMLKDCFKIILGTENKKIVKMLRGKLKKLGAISTNIEIGKRDCLDLLSTVDGLTDQQKENIVNGIFEPSKLSFYHWKELVKKNIDEVLLTEDGNLIFCWLKTISSSVKGSLKKRLKFMNIHSPELMPENCLFSSEEFNELIKLKKLLLNEQQDEQELKQDLLISSWIKCITACKDFASINDKIQKFIYHLSEELYDIRLQHLELSKLKQEHPSVSFTKEEVLIKRLEKNFLKQHNLEIMETVNLVFFAALSAPWCLHYKALESYLVRHPEILDCGSKEDCKLTLLDLSVSKLLVCLYQKDDEELINSSSLKLGFLVKYVVTLFTSNGEPFSLSLNDGGLDLDLHKTTDEKLLHQTKIVFAKIGLSGNSYDFIWTTQMIANSNFNVCKRLTGRSTGERLPRSVRSKVIYEMVKLVGETGMAILQQLAFAQALNYEHRFYAVLAPKAQLGGARDLLVQETGTKVMHATTEMFSRNLLKTTSDDGLTNPHLKETILNVGLDCLANMRNLDGKPISEGSNLVNFYKVICISGDNTKWGPIHCCSFFSGMMQQVLKNVPDWCSFYKLTFIKNLCRQVEIPAGSIKKILNVLRYRLCSKGGVEQHSEEDLRRLLTDNLDSWDGNDTVKFLVTTYISKGLMALNSYNHMGQGIHHATSSVLTSLAAVLFEELAIFYLKRSLPQTTVHVEHAGSSDDYAKCIVVTGILSKELYSQYDETFWKHACRLKNFTAAVQRCCQMKDSAKTLVSDCFLEFYSEFMMGYRVTPAVIKFMFTGLINSSVTSPQSLMQACQVSSQQAMYNSVPLVTNTAFTLLRQQIFFNHVEDFIRRYGILTLGTLSPFGRLFVPTYSGLVSSAVALEDAEVIARAAQTLQMNSVSIQSSSLTTLDSLGRSRTSSTAEDSSSVSDTTAASHDSGSSSSSFSFELNRPLSETELQFIKALSSLKSTQACEVIQNRITGLYCNSNEGPLDRHNVIYSSRMADSCDWLKDGKRRGNLELANRIQSVLCILIAGYYRSFGGEGTEKQVKASLNRDDNKIIEDPMIQLIPEKLRRELERLGVSRMEVDELMPSISPDDTLAQLVAKKLISLNVSTEEYSAEVSRLKQTLTARNVLHGLAGGIKELSLPIYTIFMKSYFFKDNVFLSLTDRWSTKHSTNYRDSCGKQLKGRIITKYTHWLDTFLGCSVSINRHTTVKEPSLFNPNIRCVNLITFEDGLRELSVIQSHLKVFENEFTNLNLQFSDPNRQKLRIVESRPAESELEANRAVIVKTKLFSATEQVRLSNNPAVVMGYLLDESAISEVKPTKVDFSNLLKDRFKIMQFFPSVFTLIKMLTDESSDSEKSGLSPDLQQVARYSNHLTLLSRMIQQAKPTVTVFYMLKGNLMNTEPTVAELVSYGIKEGRFFRLSDTGVDASTYSVKYWKILHCISAIGCLPLSQADKSSLLMSFLNWRVNMDIRTSDCPLSSHEASILSEFDGQVIANILASELSSVKRDSEREGLTDLLDYLNSPTELLKKKPYLGTTCKFNTWGDSNRSGKFTYSSRSGESIGIFIAGKLHIHLSSESVALLCETERQVLSWMSKRRTEVITKEQHQLFLSLLPQSHECLQKHKDGSALSVIPDSSNPRLLKFVPLKKGLAVVKIKKQILTVKKQVVFDAESEPRLQWGHGCLSIVYDETDTQTTYHENLLKVKHLVDCSTDRKKLLPQSVFSDSKVVLSRIKFKTELLLNSLTLLHCFLKHAPSDAIMEVESKSSLLHKYLKSGGVRQRNTEVLFREKLNKVVIKDNLEQGVEEEIEFCNNLTKTVSENPLPLSCWSEVQNYIEDIGFNNVLVNIDRNTVKSELLWKFTLDTNVSTTSTIKDVRTLVSYVSTETIPKFLLAFLLYEEVLMNLINQCKAVKELINSTGLSDLELESLLTLCAFYFQSECSKRDGPRCSFAALLSLIHEDWQRIGKNILVRANNELGDVSLKVNIVLVPLKDMSKPKSERVVMARRSLNHALSLMFLDEMSLPELKSLSVNCKMGNFEGQECFEFTILKDNSARLDYNKLIDHCVDMEKKREAVRAVEDLILMLTGRAVKPSAVTQFVHGDEQCQEQISLDDLMANDTVTDFPDREAEALKTGNLGFNWDSD DIP protein Q6TQR6 original sequence version: 44 DIP protein Q6TQR6 original sequence version: 48 0F95F82432B0DBE1 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG isg15_human Ubiquitin-like protein ISG15 ISG15 G1P2 UCRP Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Ubiquitin cross-reactive protein protein protein human Homo sapiens Human MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS DIP protein P05161 original sequence version: 130 DIP protein P05161 original sequence version: 142 DIP protein P05161 original sequence version: 155 DIP protein P05161 original sequence version: 96 DIP protein P05161 original sequence version: 141 DIP protein P05161 original sequence version: 168 DIP protein P05161 original sequence version: 150 B6858A15AB0FFFDE otu1_yeast Ubiquitin thioesterase OTU1 OTU1 OTU domain-containing protein 1 YFL044C protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLKVTGAGINQVVTLKQDATLNDLIEHINVDVKTMRFGYPPQRINLQGEDASLGQTQLDELGINSGEKITIESSDSNESFSLPPPQPKPKRVLKSTEMSIGGSGENVLSVHPVLDDNSCLFHAIAYGIFKQDSVRDLREMVSKEVLNNPVKFNDAILDKPNKDYAQWILKMESWGGAIEIGIISDALAVAIYVVDIDAVKIEKFNEDKFDNYILILFNGIHYDSLTMNEFKTVFNKNQPESDDVLTAALQLASNLKQTGYSFNTHKAQIKCNTCQMTFVGEREVARHAESTGHVDFGQNR 32ABA3CFEA4BB5EB 32ABA3CFEA4BB5EB l-ub-1 7 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 1-185 Region 1-185 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction isg15-l-1 2 10 unspecified role unspecified role unspecified role unspecified role Region 1-169 Region 1-169 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role Region 1-156 Region 1-156 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction l-ub-2 5 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 1-185 Region 1-185 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction l-ub-3 3 11 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal 10 enzyme enzyme unspecified role unspecified role Region 1-185 Region 1-185 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q6TQR6:p.Val12Thr V12T mutation mutation certain certain sequence position certain certain certain sequence position certain V T This feature has been corrected as a result of our quality control procedures. The original label was 'V12T'. Wed May 02 16:18:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val12thr' Tue Jun 30 18:57:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val12Thr' Q6TQR6:p.Ile131Asn I131N mutation mutation certain certain sequence position certain certain certain sequence position certain I N This feature has been corrected as a result of our quality control procedures. The original label was 'I131N'. Wed May 02 15:27:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile131asn' Tue Jun 30 19:43:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile131Asn' Q6TQR6:p.Ala129Ser A129S mutation mutation certain certain sequence position certain certain certain sequence position certain A S This feature has been corrected as a result of our quality control procedures. The original label was 'A129S'. Wed May 02 17:31:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala129ser' Tue Jun 30 20:07:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala129Ser' Q6TQR6:p.Glu128Ala Q128A mutation mutation certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:18:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu128ala' Tue Jun 30 19:54:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu128Ala' Q6TQR6:p.Gln16Ser Q16S mutation mutation certain certain sequence position certain certain certain sequence position certain Q S This feature has been corrected as a result of our quality control procedures. The original label was 'Q16S'. Wed May 02 16:26:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln16ser' Tue Jun 30 20:22:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln16Ser' ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction isg15-l-2 4 10 unspecified role unspecified role unspecified role unspecified role Region1-169 Region1-169 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role Region 1-156 Region 1-156 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction l-ub-4 6 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 1-185 Region 1-185 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction isg15-l-3 8 10 enzyme enzyme unspecified role unspecified role Region 1-185 Region 1-185 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q6TQR6:p.Val12Thr V12T mutation mutation certain certain sequence position certain certain certain sequence position certain V T This feature has been corrected as a result of our quality control procedures. The original label was 'V12T'. Wed May 02 15:32:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val12thr' Tue Jun 30 19:18:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val12Thr' Q6TQR6:p.Ile131Asn I131N mutation mutation certain certain sequence position certain certain certain sequence position certain I N This feature has been corrected as a result of our quality control procedures. The original label was 'I131N'. Wed May 02 18:58:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile131asn' Tue Jun 30 17:21:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile131Asn' Q6TQR6:p.Ala129Ser A129S mutation mutation certain certain sequence position certain certain certain sequence position certain A S This feature has been corrected as a result of our quality control procedures. The original label was 'A129S'. Wed May 02 16:12:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala129ser' Tue Jun 30 17:29:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala129Ser' Q6TQR6:p.Glu128Ala Q128A mutation mutation certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:57:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu128ala' Tue Jun 30 20:06:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu128Ala' Q6TQR6:p.Gln16Ser Q16S mutation mutation certain certain sequence position certain certain certain sequence position certain Q S This feature has been corrected as a result of our quality control procedures. The original label was 'Q16S'. Wed May 02 17:28:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln16ser' Tue Jun 30 17:38:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln16Ser' ecolx Escherichia coli 12 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-term range C-terminal range c-term range c-term range C-terminal range c-term range enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction ub-yotu-1 1 13 enzyme enzyme unspecified role unspecified role Region 87-301 Region 87-301 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction isg15-l-4 9 10 unspecified role unspecified role unspecified role unspecified role Region 1-169 Region 1-169 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role Region 1-156 Region 1-156 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction