DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216738X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216739X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216736X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216734X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216737X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216735X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS biology 2010 imex curation Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Dorwart MR., Wray R., Brautigam CA., Jiang Y., Blount P. DIP original experiment: DIP-216740X PLoS biology 2010 IMEx S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. mscl_ecoli Large-conductance mechanosensitive channel mscL b3291 JW3252 yhdC protein protein ecoli Escherichia coli (strain K12) MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS 18EF7E1BD7DF9491 18EF7E1BD7DF9491 mscl_staas Large-conductance mechanosensitive channel mscL SAS1287 protein protein staas Staphylococcus aureus (strain MSSA476) MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK DIP protein Q6G9L1 original sequence version: 44 mscl-8 4 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction mscl-9 1 9 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction mscl-10 6 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction mscl-11 2 9 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q6G9L1:p.[Leu10Cys;Met91Cys] L10C/M91C mutation mutation certain certain sequence position certain certain certain sequence position certain M C certain certain sequence position certain certain certain sequence position certain L C This feature has been corrected as a result of our quality control procedures. The original label was 'L10C/M91C'. Wed May 02 17:07:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu10cys,met91cys' Tue Jun 30 19:59:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu10Cys;Met91Cys]' ecolx Escherichia coli physical association physical association mscl-12 3 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction mscl-13 5 9 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q6G9L1:p.[Leu10Cys;Leu90Cys] L10C/L90C mutation mutation certain certain sequence position certain certain certain sequence position certain L C certain certain sequence position certain certain certain sequence position certain L C This feature has been corrected as a result of our quality control procedures. The original label was 'L10C/L90C'. Wed May 02 15:43:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu10cys,leu90cys' Tue Jun 30 19:40:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu10Cys;Leu90Cys]' ecolx Escherichia coli physical association physical association mscl-14 7 8 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction