DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Design, function and structure of a monomeric ClC transporter. Design, function and structure of a monomeric ClC transporter. Nature 2010 imex curation Robertson JL., Kolmakova-Partensky L., Miller C. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Robertson JL., Kolmakova-Partensky L., Miller C. DIP original experiment: DIP-219266X Nature 2010 IMEx Design, function and structure of a monomeric ClC transporter. Design, function and structure of a monomeric ClC transporter. Design, function and structure of a monomeric ClC transporter. Nature 2010 imex curation Robertson JL., Kolmakova-Partensky L., Miller C. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Robertson JL., Kolmakova-Partensky L., Miller C. DIP original experiment: DIP-219268X Nature 2010 IMEx Design, function and structure of a monomeric ClC transporter. Design, function and structure of a monomeric ClC transporter. Design, function and structure of a monomeric ClC transporter. Nature 2010 imex curation Robertson JL., Kolmakova-Partensky L., Miller C. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Robertson JL., Kolmakova-Partensky L., Miller C. DIP original experiment: DIP-219267X Nature 2010 IMEx Design, function and structure of a monomeric ClC transporter. clca_ecoli H(+)/Cl(-) exchange transporter ClcA clcA ClC-ec1 eriC yadQ JW5012 b0155 protein protein ecoli Escherichia coli (strain K12) MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGTLVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAPEAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGRMVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAVFIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGMQDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLVFIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIAGMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILARTLAKQEAEQLARSKAASASENT 0B1BC39B417E9690 clca-1 1 4 unspecified role unspecified role unspecified role unspecified role P37019:p.Ile422Trp I422W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I W This feature has been corrected as a result of our quality control procedures. The original label was 'I422W'. Wed May 02 18:08:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile422trp' Tue Jun 30 18:54:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile422Trp' P37019:p.[Ile201Trp;Ile422Trp] I201W/I422W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I W certain certain sequence position certain certain certain sequence position certain I W This feature has been corrected as a result of our quality control procedures. The original label was 'I201W/I422W'. Wed May 02 19:41:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile201trp,ile422trp' Tue Jun 30 19:59:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile201Trp;Ile422Trp]' P37019:p.Ile201Trp I201W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I W This feature has been corrected as a result of our quality control procedures. The original label was 'I201W'. Wed May 02 15:25:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile201trp' Tue Jun 30 18:10:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile201Trp' P37019:p.Leu434Trp I434W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L W Wed May 02 15:37:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu434trp' Tue Jun 30 17:46:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu434Trp' P37019:p.Leu406Trp L406W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L W This feature has been corrected as a result of our quality control procedures. The original label was 'L406W'. Wed May 02 17:54:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu406trp' Tue Jun 30 16:54:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu406Trp' ecolx Escherichia coli direct interaction direct interaction clca-2 3 4 unspecified role unspecified role unspecified role unspecified role P37019:p.[Ile201Trp;Ile422Trp] I201W/I422W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I W certain certain sequence position certain certain certain sequence position certain I W This feature has been corrected as a result of our quality control procedures. The original label was 'I201W/I422W'. Wed May 02 20:04:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile201trp,ile422trp' Tue Jun 30 16:59:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile201Trp;Ile422Trp]' ecolx Escherichia coli direct interaction direct interaction clca-3 2 4 unspecified role unspecified role unspecified role unspecified role P37019:p.[Ile201Trp;Ile422Trp] I201W/I422W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I W certain certain sequence position certain certain certain sequence position certain I W This feature has been corrected as a result of our quality control procedures. The original label was 'I201W/I422W'. Wed May 02 20:32:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile201trp,ile422trp' Tue Jun 30 17:27:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile201Trp;Ile422Trp]' ecolx Escherichia coli direct interaction direct interaction