DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220573X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yim H., Erikson RL. DIP original experiment: DIP-220569X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Yim H., Erikson RL. DIP original experiment: DIP-220576X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yim H., Erikson RL. DIP original experiment: DIP-220577X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220572X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Yim H., Erikson RL. DIP original experiment: DIP-220574X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220571X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Yim H., Erikson RL. DIP original experiment: DIP-220575X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yim H., Erikson RL. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yim H., Erikson RL. DIP original experiment: DIP-220570X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase. cdc6_human Cell division control protein 6 homolog CDC6 CDC18L CDC6-related protein p62(cdc6) Cdc18-related protein protein protein human Homo sapiens Human MPQTRSQAQATISFPKRKLSRALNKAKNSSDAKLEPTNVQTVTCSPRVKALPLSPRKRLGDDNLCNTPHLPPCSPPKQGKKENGPPHSHTLKGRRLVFDNQLTIKSPSKRELAKVHQNKILSSVRKSQEITTNSEQRCPLKKESACVRLFKQEGTCYQQAKLVLNTAVPDRLPAREREMDVIRNFLREHICGKKAGSLYLSGAPGTGKTACLSRILQDLKKELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSPSEPLIPKRVGLIHISQVISEVDGNRMTLSQEGAQDSFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP DIP protein Q99741 original sequence version: 102 3ED7DE4AF80CB017 cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM DIP protein P06493 original sequence version: 185 DIP protein P06493 original sequence version: 162 DIP protein P06493 original sequence version: 184 DIP protein P06493 original sequence version: 97 DIP protein P06493 original sequence version: 192 942D79448EFE490A plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 cdc6-cdk1-1 7 11 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait Q99741:p.Thr37Val T37V mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T V This feature has been corrected as a result of our quality control procedures. The original label was 'T37V'. Wed May 02 17:26:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr37val' Tue Jun 30 19:26:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr37Val' GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cdc6-plk1-1 2 12 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminal domain Region 306-603 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P53350:p.[Trp414Phe;Val415Ala;Leu427Ala] W414F/V415A/L427A/ mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain W F This feature has been corrected as a result of our quality control procedures. The original label was 'W414F/V415A/L427A/'. Wed May 02 15:28:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp414phe,val415ala,leu427ala' Tue Jun 30 19:32:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp414Phe;Val415Ala;Leu427Ala]' human Homo sapiens Human 10 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association cdc6-plk1-2 1 12 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association cdc6-plk1-3 8 12 enzyme enzyme unspecified role unspecified role P53350:p.Lys82Met K82M mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K M This feature has been corrected as a result of our quality control procedures. The original label was 'K82M'. Wed May 02 16:36:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys82met' Tue Jun 30 17:10:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys82Met' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation cdc6-plk1-4 6 12 unspecified role unspecified role prey prey C-terminal domain Region 306-603 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 12 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human association association cdc6-plk1-5 3 12 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-100 Region 1-100 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q99741:p.Thr37Val T37V mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T V This feature has been corrected as a result of our quality control procedures. The original label was 'T37V'. Wed May 02 16:55:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr37val' Tue Jun 30 17:30:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr37Val' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation cdc6-cdk1-2 5 11 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait Q99741:p.Thr37Val T37V mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T V This feature has been corrected as a result of our quality control procedures. The original label was 'T37V'. Wed May 02 19:27:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr37val' Tue Jun 30 20:11:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr37Val' human Homo sapiens Human physical association physical association plk1-cdc6-1 9 12 unspecified role unspecified role bait bait human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association plk1-1 4 12 putative self putative self unspecified role unspecified role P53350:p.Lys82Met K82M mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K M This feature has been corrected as a result of our quality control procedures. The original label was 'K82M'. Wed May 02 15:21:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys82met' Tue Jun 30 19:54:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys82Met' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation true