DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structure (London, England : 1993) 2010 imex curation Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. Only protein-protein interactions ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. DIP original experiment: DIP-220697X Structure (London, England : 1993) 2010 IMEx Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structure (London, England : 1993) 2010 imex curation Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. DIP original experiment: DIP-220695X Structure (London, England : 1993) 2010 IMEx Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structure (London, England : 1993) 2010 imex curation Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. DIP original experiment: DIP-220693X Structure (London, England : 1993) 2010 IMEx Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structure (London, England : 1993) 2010 imex curation Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. DIP original experiment: DIP-220694X Structure (London, England : 1993) 2010 IMEx Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. Structure (London, England : 1993) 2010 imex curation Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. Only protein-protein interactions ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Cheng Z., He YW., Lim SC., Qamra R., Walsh MA., Zhang LH., Song H. DIP original experiment: DIP-220696X Structure (London, England : 1993) 2010 IMEx Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis. rpfc_xancp Sensory/regulatory protein RpfC rpfC XCC1856 protein protein strain atcc 33913 / Xanthomonas campestris pv. campestris MKSPLPWLKRRLSGRADSEHAQNLIRIIITTLFISYLGWRYQHTHGDTLMATWLILVGELLVSLGLMVAILLRPQVSHTRRLIGMLLDYTCTGAIMAIQGEPASPLYAVCMWVTIGNGLRYGSNYLRAATAMGSLCFLGAILISPYWKANPYLSWGLLLGLIAVPLYFDSLLRAMTRAVREARHANQAKSRFLANMSHEFRTPLNGLSGMTEVLATTRLDAEQKECLNTIQASARSLLSLVEEVLDISAIEAGKIRIDRRDFSLREMIGSVNLILQPQARGRRLEYGTQVADDVPDLLKGDTAHLRQVLLNLVGNAVKFTEHGHVLLRVTRVSGSAEDAVRLRFDVEDTGIGVPMDMRPRLFEAFEQADVGLSRRYEGTGLGTTIAKGLVEAMGGSIGFKENQPSGSVFWFELPMAIGEPLKSSTVRVPTGALVDAPEELESSNIIAFSNPFLRHRARVRSMRMLVADDHEANRMVLQRLLEKAGHKVLCVNGAEQVLDAMAEEDYDAVIVDLHMPGMNGLDMLKQLRVMQASGMRYTPVVVLSADVTPEAIRACEQAGARAFLAKPVVAAKLLDTLADLAVSTRQLATPATTVQVATSFEGVLDSSVLDELAALGMGEEFERQFVRQCLDDAQNCVGDIERDGTCSDWEQLRESAHALRGVASNLGLAQVASSGGELMRMADWQLQAEWRLRLSTLREQLKAGKDALDARVQGVKDGECSPRSNE DIP protein P0C0F6 original sequence version: 55 q7cls3_xancp rpfF XCC1857 protein protein strain atcc 33913 / Xanthomonas campestris pv. campestris MSAVQPFIRTNIGSTLRIIEEPQRDVYWIHMHADLAINPGRACFSTRLVDDITGYQTNLGQRLNTAGVLAPHVVLASDSDVFNLGGDLALFCQLIREGDRARLLDYAQRCVRGVHAFHVGLGARAHSIALVQGNALGGGFEAALSCHTIIAEEGVMMGLPEVLFDLFPGMGAYSFMCQRISAHLAQKIMLEGNLYSAEQLLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVREMTTAVPLEEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQSRRSGLDAG DIP protein Q7CLS3 original sequence version: 34 rpff-rpfc-1 3 6 unspecified role unspecified role prey prey CheY-like receiver domain Region 449-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association rpfc-rpff-1 2 6 unspecified role unspecified role unspecified role unspecified role CheY-like receiver domain Region 449-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rpff-rpfc-2 5 6 unspecified role unspecified role prey prey CheY-like receiver domain Region 449-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait Q7CLS3:p.[Leu136Ala;Leu194Ala] L136A/L194A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L136A/L194A'. Wed May 02 19:03:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu136ala,leu194ala' Tue Jun 30 18:19:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu136Ala;Leu194Ala]' ecolx Escherichia coli direct interaction direct interaction rpfc-rpff-2 4 6 unspecified role unspecified role unspecified role unspecified role CheY-like receiver domain Region 449-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rpff-rpfc-3 1 6 unspecified role unspecified role prey prey CheY-like receiver domain Region 449-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P0C0F6:p.[Glu495Ala;Leu498Ala;Asp499Ala] E495A/L498A/D499A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E495A/L498A/D499A'. Wed May 02 16:18:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu495ala,leu498ala,asp499ala' Tue Jun 30 20:06:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu495Ala;Leu498Ala;Asp499Ala]' P0C0F6:p.[Arg528Ala;Val529Ala;Met530Ala] R528A/V529A/M530A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R528A/V529A/M530A'. Wed May 02 16:30:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg528ala,val529ala,met530ala' Tue Jun 30 19:03:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg528Ala;Val529Ala;Met530Ala]' ecolx Escherichia coli 7 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction