DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219477X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219476X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219472X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219479X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219480X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219478X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219474X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219473X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219475X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. Nature 2010 imex curation Lin SC., Lo YC., Wu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lin SC., Lo YC., Wu H. DIP original experiment: DIP-219481X Nature 2010 IMEx Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling. q99836-1 Myeloid differentiation primary response protein MyD88 MYD88 protein protein human Homo sapiens Human MAAGGPGAGSAAPVSSTSSLPLAALNMRVRRRLSLFLNVRTQVAADWTALAEEMDFEYLEIRQLETQADPTGRLLDAWQGRPGASVGRLLELLTKLGRDDVLLELGPSIEEDCQKYILKQQQEEAEKPLQVAAVDSSVPRTAELAGITTLDDPLGHMPERFDAFICYCPSDIQFVQEMIRQLEQTNYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDDYLQSKECDFQTKFALSLSPGAHQKRLIPIKYKAMKKEFPSILRFITVCDYTNPCTKSWFWTRLAKALSLP DIP protein Q99836-1 original sequence version: 100 DIP protein Q99836-1 original sequence version: 128 DIP protein Q99836 original sequence version: 128 DIP protein Q99836-1 original sequence version: 156 irak4_human Interleukin-1 receptor-associated kinase 4 Renal carcinoma antigen NY-REN-64 IRAK4 protein protein human Homo sapiens Human MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS DIP protein Q9NWZ3 original sequence version: 93 DIP protein Q9NWZ3 original sequence version: 92 DIP protein Q9NWZ3 original sequence version: 144 6C8156ADF25FF81E irak2_human Interleukin-1 receptor-associated kinase-like 2 IRAK2 protein protein human Homo sapiens Human MACYIYQLPSWVLDDLCRNMDALSEWDWMEFASYVITDLTQLRKIKSMERVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFPGPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEIIHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDDVDNSSLDASSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP DIP protein O43187 original sequence version: 90 715C954BC9452710 irak4-myd88-8 1 12 unspecified role unspecified role bait bait Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 11 unspecified role unspecified role prey prey Death domain Region 20-154 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association irak2-irak4-1 3 12 unspecified role unspecified role unspecified role unspecified role Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Death domain Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Death domain Region 20-117 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association irak4-irak2-1 2 12 unspecified role unspecified role bait bait Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 13 unspecified role unspecified role prey prey Death domain Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role prey prey Death domain Region 20-154 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association irak4-myd88-9 7 12 unspecified role unspecified role unspecified role unspecified role Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Death domain Region 20-117 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction irak2-irak4-2 8 12 unspecified role unspecified role unspecified role unspecified role Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Death domain Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Death domain Region 20-117 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association irak4-irak2-2 10 12 unspecified role unspecified role bait bait Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9NWZ3:p.Gln50Lys Q50K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q K This feature has been corrected as a result of our quality control procedures. The original label was 'Q50K'. Wed May 02 15:27:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln50lys' Tue Jun 30 18:38:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln50Lys' Q9NWZ3:p.Arg54Asp R54D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R54D'. Wed May 02 15:42:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg54asp' Tue Jun 30 19:41:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg54Asp' Q9NWZ3:p.Ala95Lys A95K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A K This feature has been corrected as a result of our quality control procedures. The original label was 'A95K'. Wed May 02 16:52:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala95lys' Tue Jun 30 19:24:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala95Lys' Q9NWZ3:p.Phe25Asp F25D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F D This feature has been corrected as a result of our quality control procedures. The original label was 'F25D'. Wed May 02 16:06:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe25asp' Tue Jun 30 15:56:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe25Asp' Q9NWZ3:p.Phe51Asp F51D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F D This feature has been corrected as a result of our quality control procedures. The original label was 'F51D'. Wed May 02 18:05:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe51asp' Tue Jun 30 20:20:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe51Asp' ecolx Escherichia coli 13 unspecified role unspecified role prey prey Death domain Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association irak4-myd88-10 9 12 unspecified role unspecified role unspecified role unspecified role Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Death domain Region 20-117 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association myd88-irak4-7 4 12 unspecified role unspecified role prey prey Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role bait bait Death domain Region 20-117 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q99836-1:p.Val43Asp V43D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V43D'. Wed May 02 17:17:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val43asp' Tue Jun 30 20:12:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val43Asp' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q99836-1:p.Glu52Lys E52K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E52K'. Wed May 02 19:13:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu52lys' Tue Jun 30 18:54:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu52Lys' Q99836-1:p.Asp55Ala D55A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D55A'. Wed May 02 17:44:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp55ala' Tue Jun 30 17:21:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp55Ala' Q99836-1:p.Ile61Ala I61A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I61A'. Wed May 02 15:32:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile61ala' Tue Jun 30 18:16:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile61Ala' Q99836-1:p.Arg62Glu R62E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R62E'. Wed May 02 17:52:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg62glu' Tue Jun 30 15:57:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg62Glu' Q99836-1:p.Ala44Glu A44E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A E This feature has been corrected as a result of our quality control procedures. The original label was 'A44E'. Wed May 02 16:49:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala44glu' Tue Jun 30 19:29:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala44Glu' Q99836-1:p.[Tyr58Ala;Arg62Ala] Y58A/R62A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y58A/R62A'. Wed May 02 14:57:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr58ala,arg62ala' Tue Jun 30 20:01:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr58Ala;Arg62Ala]' ecolx Escherichia coli physical association physical association irak4-myd88-11 6 12 unspecified role unspecified role bait bait Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9NWZ3:p.Glu69Lys E69K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E69K'. Wed May 02 14:29:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu69lys' Tue Jun 30 17:31:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu69Lys' Q9NWZ3:p.Thr76Glu T76E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T E This feature has been corrected as a result of our quality control procedures. The original label was 'T76E'. Wed May 02 19:49:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr76glu' Tue Jun 30 17:51:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr76Glu' Q9NWZ3:p.Asn78Glu N78E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N E This feature has been corrected as a result of our quality control procedures. The original label was 'N78E'. Wed May 02 15:28:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn78glu' Tue Jun 30 18:14:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn78Glu' Q9NWZ3:p.Arg12Ala R12A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R12A'. Wed May 02 15:10:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg12ala' Tue Jun 30 17:30:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg12Ala' Q9NWZ3:p.[Val16Glu;Arg20Glu] V16E/R20E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain V E This feature has been corrected as a result of our quality control procedures. The original label was 'V16E/R20E'. Wed May 02 17:04:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val16glu,arg20glu' Tue Jun 30 16:15:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val16Glu;Arg20Glu]' ecolx Escherichia coli 11 unspecified role unspecified role prey prey Death domain Region 20-154 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association irak2-irak4-3 5 12 unspecified role unspecified role prey prey Death domain Region 4-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 13 unspecified role unspecified role bait bait Death domain Region 1-112 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O43187:p.Trp62Asp W62D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W D This feature has been corrected as a result of our quality control procedures. The original label was 'W62D'. Wed May 02 15:13:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp62asp' Tue Jun 30 17:06:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp62Asp' O43187:p.Met66Asp M66D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M D This feature has been corrected as a result of our quality control procedures. The original label was 'M66D'. Wed May 02 15:41:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met66asp' Tue Jun 30 19:57:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met66Asp' O43187:p.Arg67Asp R67D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R67D'. Wed May 02 16:17:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg67asp' Tue Jun 30 16:42:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg67Asp' O43187:p.Tyr6Asp Y6D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y6D'. Wed May 02 14:50:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr6asp' Tue Jun 30 18:37:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr6Asp' ecolx Escherichia coli 11 unspecified role unspecified role prey prey Death domain Region 20-154 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association