DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010 imex curation Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. DIP original experiment: DIP-220724X Nature 2010 IMEx Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010 imex curation Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. Only protein-protein interactions ecolx Escherichia coli scanning electron microscopy scanning electron microscopy sem sem predetermined predetermined participant predetermined Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. DIP original experiment: DIP-220728X Nature 2010 IMEx Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010 imex curation Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. DIP original experiment: DIP-220725X Nature 2010 IMEx Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010 imex curation Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. DIP original experiment: DIP-220727X Nature 2010 IMEx Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010 imex curation Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. Only protein-protein interactions ecolx Escherichia coli fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. DIP original experiment: DIP-220726X Nature 2010 IMEx Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010 imex curation Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight SD. DIP original experiment: DIP-220723X Nature 2010 IMEx Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. q05h60_9arac MaSp1 protein protein euprosthenops australis Euprosthenops australis MSWTARLALLLLFVACQGSSSLASHTTPWTNPGLAENFMNSFMQGLSSMPGFTASQLDDMSTIAQSMVQSIQSLAAQGRTSPNKLQALNMAFASSMAEIAASEEGGGSLSTKTSSIASAMSNAFLQTTGVVNQPFINEITQLVSMFAQAGMNDVSASASAGASAAASAGAPGYSPAPSYSSGGYASSAASAAAAAGQGGPGGYGPAPNQGASSAAAAAAGSGQGPSGPYGTSYQISTQYTQTTTSQGQGYGSSSAGAAAAGAAGAGQGGYGGQGQGGYGQGAGGAAAAAAAAAAAAAAAGQGGQGGGGYGQGGQGGQGGQGQGGYGQGAGSSAAAAAAAAAAAAAAGRGQGGYGQGSGGAAAAAGSGQGGYGGRRQGGYGQAAAAGQGGQGGQG DIP protein Q05H60 original sequence version: 6 masp1-1 6 7 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 29-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction masp1-2 5 7 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 29-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction masp1-3 4 7 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 29-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction masp1-4 1 7 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region29-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction masp1-5 3 7 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 29-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction masp1-6 2 7 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 29-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction