DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219782X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219781X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219780X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219778X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Boudreaux DA., Maiti TK., Davies CW., Das C. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Boudreaux DA., Maiti TK., Davies CW., Das C. DIP original experiment: DIP-219779X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation. ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 uchl1_human Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Neuron cytoplasmic protein 9.5 PGP 9.5 UCHL1 protein protein human Homo sapiens Human MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA DIP protein P09936 original sequence version: 135 C9E972AC4DA5DA8A hub-uchl1-1 4 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction hub-uchl1-2 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label fluorescent dye fluorescent dye label fluorescent dye c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal ecolx Escherichia coli 8 enzyme enzyme unspecified role unspecified role P09936:p.Phe214Ala F214A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F214A'. Wed May 02 18:35:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe214ala' Tue Jun 30 20:15:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe214Ala' P09936:p.Cys90Ser C90S mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C90S'. Wed May 02 16:51:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys90ser' Tue Jun 30 15:39:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys90Ser' ecolx Escherichia coli protein cleavage protein cleavage hub-uchl1-3 5 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction hub-uchl1-4 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction bub-uchl1-1 3 7 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 8 unspecified role unspecified role unspecified role unspecified role P09936:p.Phe214Ala F214A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F214A'. Wed May 02 19:30:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe214ala' Tue Jun 30 17:32:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe214Ala' ecolx Escherichia coli direct interaction direct interaction