DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. DIP original experiment: DIP-216394X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. DIP original experiment: DIP-216392X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. DIP original experiment: DIP-216393X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. DIP original experiment: DIP-216391X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rosario CO., Ko MA., Haffani YZ., Gladdy RA., Paderova J., Pollett A., Squire JA., Dennis JW., Swallow CJ. DIP original experiment: DIP-216390X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Plk4 is required for cytokinesis and maintenance of chromosomal stability. ect2_human Protein ECT2 Epithelial cell-transforming sequence 2 oncogene ECT2 protein protein human Homo sapiens Human MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKSDEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSDELPKENWLKMLCRHVANTICKADAENLIYTADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALMTSHGSVEGRSPSSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI DIP protein Q9H8V3 original sequence version: 73 DIP protein Q9H8V3 original sequence version: 72 DIP protein Q9H8V3-1 original sequence version: 72 DIP protein Q9H8V3 original sequence version: 130 F40FC4F982FB8C78 rhoa_human Transforming protein RhoA Rho cDNA clone 12 ARHA RHO12 RHOA ARH12 protein protein human Homo sapiens Human MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL DIP protein P61586 original sequence version: 86 DIP protein P61586 original sequence version: 123 DIP protein P61586 original sequence version: 57 DIP protein P61586 original sequence version: 88 DIP protein P61586 original sequence version: 87 DIP protein P61586 original sequence version: 85 DIP protein P61586 original sequence version: 125 DIP protein P61586 original sequence version: 132 C4DA2DC31FF858BC rtkn_mouse Rhotekin Rtkn protein protein mouse Mus musculus Mouse MFSRNHRSRITVARGSALEMEFKRGRFRLSFFSESPEDTELQRKLDHEIRMRDGACKLLAACSQREQALEATKSLLVCNSRILSYMGELQRRKEAQVLEKTGRRPSDSVQPAQHSPCRGRVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQIGEQIQDTEMVLVDRTLTDISFQNNVLFAEAEPDFELRLELYGACVEEEGALAGAPKRLATKLSSSLGRSSGKRVRASLDSAGASGNSPVLLPTPAVGGPRFHLLAHTTLTLEEVQDGFRTHDLTLTSHEENPAWLPLYGSVCCRLAAQPLCMIQPTASGALRVQQAGELQNGTLVHGVLKGTNLFCYWRSEDADTGQEPLFTIVINKETRVRAGELEQAPEWPFTLSISNKYGDDEVTNTLQLESREALQNWMEALWQLFFDMSQWRHCCDEVMKIETPAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILAQREGTRLEPSPPWLAMFTDQPALPSSCSPASVAPVPTWMQPLPWGRPRTFSLDAAPADHSLGPSRSVAPLPPQRSPKSRGFYSKSQLGPWLQSPV DIP protein Q8C6B2 original sequence version: 41 DIP protein Q8C6B2 original sequence version: 57 0934EEBF09B12A3E rhoa_mouse Transforming protein RhoA Rhoa Arha Arha2 protein protein mouse Mus musculus Mouse MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLIL DIP protein Q9QUI0 original sequence version: 76 DIP protein Q9QUI0 original sequence version: 77 DIP protein Q9QUI0 original sequence version: 100 C4C8BDC31FF858BC plk4_human Serine/threonine-protein kinase PLK4 Polo-like kinase 4 Serine/threonine-protein kinase Sak Serine/threonine-protein kinase 18 PLK4 SAK STK18 protein protein human Homo sapiens Human MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMDHANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH DIP protein O00444 original sequence version: 88 DIP protein O00444-1 original sequence version: 93 DIP protein O00444 original sequence version: 145 DIP protein O00444-1 original sequence version: 145 DIP protein O00444 original sequence version: 132 DIP protein O00444-1 original sequence version: 132 DIP protein O00444 original sequence version: 135 4D56F5FD983211A6 plk4-ect2-1 5 6 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association rtkn-rhoa-12 3 9 unspecified role unspecified role prey prey mouse Mus musculus Mouse 8 unspecified role unspecified role bait bait Rho Binding Domain Region 7-89 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association ect2-plk4-1 4 6 unspecified role unspecified role bait bait human Homo sapiens Human 10 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association ect2-plk4-2 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli 10 enzyme enzyme unspecified role unspecified role Kinase domain Region 1-390 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation rtkn-rhoa-14 1 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait Rho Binding Domain Region 7-89 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association