DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. DIP original experiment: DIP-216354X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. DIP original experiment: DIP-216358X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. DIP original experiment: DIP-216356X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. Only protein-protein interactions ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. DIP original experiment: DIP-216355X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. Only protein-protein interactions ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Volpon L., Osborne MJ., Capul AA., de la Torre JC., Borden KL. DIP original experiment: DIP-216357X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E. z_lassj RING finger protein Z Z Zinc-binding protein protein protein lasv Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) LASV MGNKQAKAPESKDSPRASLIPDATHLGPQFCKSCWFENKGLVECNNHYLCLNCLTLLLSVSNRCPICKMPLPTKLRPSAAPTAPPTGAADSIRPPPYSP DIP protein O73557 original sequence version: 59 o43432-1 Eukaryotic translation initiation factor 4 gamma 3 EIF4G3 eIF-4-gamma II protein protein human Homo sapiens Human MNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKVLESEQDKMSQGFHPERDPSDLKKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSSGDGVTFPFKPESWKPTDTEGKKQYDREFLLDFQFMPACIQKPEGLPPISDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQADDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSALQPPAPSGSTPSTPVEFDSRRTLTSRGSMGREKNDKPLPSATARPNTFMRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRESAKPEISAMSAHDKAALSEEELERKSKSIIDEFLHINDFKEAMQCVEELNAQGLLHVFVRVGVESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDFIESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAAIIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESEDN DIP protein O43432-1 original sequence version: 92 DIP protein O43432 original sequence version: 117 if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D if4g1_human Eukaryotic translation initiation factor 4 gamma 1 p220 EIF4G1 EIF4G EIF4GI EIF4F protein protein human Homo sapiens Human MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN DIP protein Q04637 original sequence version: 68 DIP protein Q04637 original sequence version: 166 DIP protein Q04637 original sequence version: 160 DIP protein Q04637-1 original sequence version: 94 DIP protein Q04637 original sequence version: 115 DIP protein Q04637 original sequence version: 119 DIP protein Q04637 original sequence version: 146 DIP protein Q04637-1 original sequence version: 148 DIP protein Q04637 original sequence version: 150 324088B60863DA34 eif4e-z-1 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction eif4e-eif4g3-2 3 7 unspecified role unspecified role unspecified role unspecified role Region 621-637 Region 621-637 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction eif4g1-eif4e-2 2 9 unspecified role unspecified role bait bait Region 606-624 Region 606-624 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 8 unspecified role unspecified role prey prey P06730:p.Gln40Ala Q40A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q40A'. Wed May 02 18:27:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln40ala' Tue Jun 30 20:03:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln40Ala' P06730:p.Glu70Ala E70A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E70A'. Wed May 02 20:16:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu70ala' Tue Jun 30 17:17:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu70Ala' P06730:p.Trp73Ala W73A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W73A'. Wed May 02 15:47:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp73ala' Tue Jun 30 16:26:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp73Ala' P06730:p.Lys65Ala K65A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K65A'. Wed May 02 17:57:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65ala' Tue Jun 30 17:58:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Ala' P06730:p.Val69Gly V69G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V G This feature has been corrected as a result of our quality control procedures. The original label was 'V69G'. Wed May 02 19:46:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val69gly' Tue Jun 30 19:40:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val69Gly' P06730:p.Asp144Ala D144A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D144A'. Wed May 02 16:37:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp144ala' Tue Jun 30 16:51:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp144Ala' ecolx Escherichia coli direct interaction direct interaction z-eif4e-1 4 8 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role bait bait O73557:p.[Cys31Ala;Cys34Ala] C31A/C34A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C31A/C34A'. Wed May 02 16:58:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys31ala,cys34ala' Tue Jun 30 19:15:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys31Ala;Cys34Ala]' O73557:p.Trp35Ala W35A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W35A'. Wed May 02 16:32:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp35ala' Tue Jun 30 18:28:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp35Ala' O73557:p.Phe36Ala F36A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F36A'. Wed May 02 19:03:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe36ala' Tue Jun 30 18:48:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe36Ala' O73557:p.Asn38Ala N38A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N38A'. Wed May 02 14:32:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn38ala' Tue Jun 30 20:05:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn38Ala' O73557:p.Lys39Ala K39A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K39A'. Wed May 02 20:11:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys39ala' Tue Jun 30 19:10:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys39Ala' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction z-eif4e-2 5 8 unspecified role unspecified role prey prey P06730:p.Gln40Ala Q40A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q40A'. Wed May 02 17:21:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln40ala' Tue Jun 30 17:45:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln40Ala' P06730:p.Glu70Ala E70A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E70A'. Wed May 02 17:22:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu70ala' Tue Jun 30 16:16:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu70Ala' P06730:p.Trp73Ala W73A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W73A'. Wed May 02 18:07:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp73ala' Tue Jun 30 18:21:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp73Ala' P06730:p.Tyr76Ala Y76A/F mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:34:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr76ala' Tue Jun 30 16:32:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr76Ala' P06730:p.Trp130Phe W130F mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W F This feature has been corrected as a result of our quality control procedures. The original label was 'W130F'. Wed May 02 15:23:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp130phe' Tue Jun 30 20:02:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp130Phe' P06730:p.Tyr76Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:29:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr76phe' Tue Jun 30 20:12:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr76Phe' ecolx Escherichia coli 6 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction