DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133933X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-262155X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133932X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133931X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133935X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133934X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. PLoS biology 2010 imex curation Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133930X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. q8kls0_cersp cheA3 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MSDAFDEMDEIWALYADDGAQALDAMEASLLALQAGEDAAAHVGPLFRAVHTFKGNSRVLGLSVVESRAHLCEDLIGLVRDAGVPMDGEIVEILLFASDTLRAMLEETAASRADVEGTGSEALMDQLRSKIARCSGAGAATAEVDAATAEERAATAEERAAATEAGAAPTETGAAATSAAEPSEAWGGSPETGAPAAAAEVLPVAGKAFPSVADILGLLDDSALPDTGEAAEVPLEAGGRPAGEGHAPAGPPPEAGPAEAPVPPAAEPARPAARLADDPVYRAIFRDMATAALTRFRAQIDAFATDPGATAQGARAEADGLCHAARQMGLDDWEAALAAFLSDSQGPERLAALVLELEALGDEPAPTPQVEEKPFFAAIAGPLAEIGRLGLRLVTGERPGPAERAAAADPLGQAALAQGYVRVAEAAQALAAAEGAEPFGTARLRLYEELAAVEALMPEAAAAAGVSPVGLLRRLCAAEAAETLAALDATLNAFRSAPLAETFGRFERLMRCVHHACAHHALDAAAQLAMSLVDLFGRAEAKGAVPDAMLVHIARGFIETIELAFEALAQGETPAMEVLDRLFEEAANVCFVSDGLVTAGAIERRLGLPADFHRVLSPESVRAAVEEMEKGQQFYIVRTDINSDDRTAEAFLDWLSGDQARSITNVTVFRGTETLFDFLISSRLGEADLIEALVRMDPSGRRLSLTQALRPRQDEPAPVEAPGEAPLAQGAAITPEMLETIGEISANQAMVQHLLTELAEADLAEATEALVRQAAGDWAAARGPVRALLEAHGARLQEIVQAGTQLTAQLAALQEETVTQRARPASTVLRPLRTFAETLARRRRREVCLTTSGDELALDLALLERLRGFLRGLVALRLDQDRGAPGTLHVSLWRDEERVTVLVQDDGAPEPDSPALADLAAEVGQAGGALRSVPLPGGGMRFHLGLPLSMVVLEGMVVGVDGMRYVLPVESIRTILQADAAAAMTISAAGGQRVLRLSETEIVAIHSLRGHPQEGHRERPWPASRRVYVVLGTGPRSVAIPVDELVGQQLVLLRPLRGVLARLTHLTGLALLAGGDVGMVLSANRICPPLPAEAG DIP protein Q8KLS0 original sequence version: 28 DIP protein Q8KLS0 original sequence version: 27 q8kls1_cersp cheY6 EBL86_09020 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MPYNVMIVDDAAMMRLYIASFIKTLPDFKVVAQAANGQEALDKLAAQPNVDLILLDIEMPVMDGMEFLRHAKLKTRAKICMLSSVAVSGSPHAARARELGADGVVAKPSGTVSHDLEEKTGGELARTMRTLMAA DIP protein Q8KLS1 original sequence version: 38 DIP protein Q8KLS1 original sequence version: 36 q53134_cersp cheY cheY1 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MPLTVLAIDDSRTIRELLREALVQAGFEVHLAIDGLDGLEKLEAAKPHAVITDINMPRMDGFGFIRAVREQPQHSALPIIVLTTESAAELKAKAREAGATAWIVKPFDEAKLVSALRRVAVA DIP protein Q53134 original sequence version: 53 DIP protein Q53134 original sequence version: 52 chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM DIP protein P0AE67 original sequence version: 95 DIP protein P0AE67 original sequence version: 56 DIP protein P0AE67 original sequence version: 96 E4B60B8A73DA14DC q8kls7_cersp Histidine kinase cheA4 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MSAPVEAGPDGGLLRVSSEKIGRLMDLVGELSLSVAETVHSPDLEGLELPAFDGAVHRLRMIVREVQDAATELRLVPVEEVFKRLRRMVRELERQTGKKIEMVFEGAETAIDKLVADQLYDPLLHVVRNSADHGLEPPEERRAAGKPETGRIVLAAAQAGSEVRISVADDGRGLSRPKILKRARERGLFGPDEEPEPATLWKVIFEPGFSTAEAVTNLSGRGVGMDVLNATMTALRGRIGVDSRDGQGSEVSLHIPVSLAFLDCIILRLGARLYALPIDAVSEIVQPKPCDLMGVGAAGGSEMLRLRGHWIPVCRLERFYGEAPSAVPPPAGQVLAVVSTATGLIALPVDEVLDRQQVVMKPLTGRLAQVRASWGCALLGTGEVALVLDGNRLAAGGAA DIP protein Q8KLS7 original sequence version: 48 DIP protein Q8KLS7 original sequence version: 46 chea3-chey6-3 3 9 phosphate acceptor phosphate acceptor unspecified role unspecified role Q8KLS1:p.Met13Ser M13S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M S This feature has been corrected as a result of our quality control procedures. The original label was 'M13S'. Wed May 02 16:07:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met13ser' Tue Jun 30 19:22:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met13Ser' Q8KLS1:p.[Met13Ser;Leu16Ser;Tyr17Met] M13S/L16S/Y17M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y M certain certain sequence position certain certain certain sequence position certain L S certain certain sequence position certain certain certain sequence position certain M S This feature has been corrected as a result of our quality control procedures. The original label was 'M13S/L16S/Y17M'. Wed May 02 19:35:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met13ser,leu16ser,tyr17met' Tue Jun 30 20:12:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met13Ser;Leu16Ser;Tyr17Met]' ecolx Escherichia coli 8 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer chea3-chey6-4 7 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction chea3-chey-2 6 10 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli 8 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer chey6-chea3-1 4 9 unspecified role unspecified role bait bait Q8KLS1:p.Met13Ser M13S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M S This feature has been corrected as a result of our quality control procedures. The original label was 'M13S'. Wed May 02 20:14:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met13ser' Tue Jun 30 20:00:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met13Ser' Q8KLS1:p.[Met13Ser;Leu16Ser;Tyr17Met] M13S/L16S/Y17M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y M certain certain sequence position certain certain certain sequence position certain L S certain certain sequence position certain certain certain sequence position certain M S This feature has been corrected as a result of our quality control procedures. The original label was 'M13S/L16S/Y17M'. Wed May 02 15:24:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met13ser,leu16ser,tyr17met' Tue Jun 30 19:14:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met13Ser;Leu16Ser;Tyr17Met]' ecolx Escherichia coli 8 unspecified role unspecified role prey prey P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction chea3-chea4-2 5 12 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation chea3-chey-3 1 11 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli 8 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer chea3-chey-4 2 11 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli 8 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer 1 nodeMerge04 2014-11-28