DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yan J., Pan L., Chen X., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Yan J., Pan L., Chen X., Wu L., Zhang M. DIP original experiment: DIP-216306X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yan J., Pan L., Chen X., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yan J., Pan L., Chen X., Wu L., Zhang M. DIP original experiment: DIP-216305X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yan J., Pan L., Chen X., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yan J., Pan L., Chen X., Wu L., Zhang M. DIP original experiment: DIP-216308X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yan J., Pan L., Chen X., Wu L., Zhang M. Only protein-protein interactions ecolx Escherichia coli nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Yan J., Pan L., Chen X., Wu L., Zhang M. DIP original experiment: DIP-216310X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yan J., Pan L., Chen X., Wu L., Zhang M. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Yan J., Pan L., Chen X., Wu L., Zhang M. DIP original experiment: DIP-216309X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Yan J., Pan L., Chen X., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Yan J., Pan L., Chen X., Wu L., Zhang M. DIP original experiment: DIP-216307X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. ush1c_human Harmonin Usher syndrome type-1C protein Autoimmune enteropathy-related antigen AIE-75 Antigen NY-CO-38/NY-CO-37 Protein PDZ-73 Renal carcinoma antigen NY-REN-3 USH1C AIE75 protein protein human Homo sapiens Human MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEAVNVLKSSRSLTISIVAAAGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPELEPADDLDGGTEEQGEQDFRKYEEGFDPYSMFTPEQIMGKDVRLLRIKKEGSLDLALEGGVDSPIGKVVVSAVYERGAAERHGGIVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF DIP protein Q9Y6N9 original sequence version: 95 DIP protein Q9Y6N9 original sequence version: 91 7E75CEE873C57F41 ush1g_human Usher syndrome type-1G protein USH1G SANS Scaffold protein containing ankyrin repeats and SAM domain protein protein human Homo sapiens Human MNDQYHRAARDGYLELLKEATRKELNAPDEDGMTPTLWAAYHGNLESLRLIVSRGGDPDKCDIWGNTPLHLAASNGHLHCLSFLVSFGANIWCLDNDYHTPLDMAAMKGHMECVRYLDSIAAKQSSLNPKLVGKLKDKAFREAERRIRECAKLQRRHHERMERRYRRELAERSDTLSFSSLTSSTLSRRLQHLALGSHLPYSQATLHGTARGKTKMQKKLERRKQGGEGTFKVSEDGRKSARSLSGLQLGSDVMFVRQGTYANPKEWGRAPLRDMFLSDEDSVSRATLAAEPAHSEVSTDSGHDSLFTRPGLGTMVFRRNYLSSGLHGLGREDGGLDGVGAPRGRLQSSPSLDDDSLGSANSLQDRSCGEELPWDELDLGLDEDLEPETSPLETFLASLHMEDFAALLRQEKIDLEALMLCSDLDLRSISVPLGPRKKILGAVRRRRQAMERPPALEDTEL DIP protein Q495M9 original sequence version: 53 mint Unknown. Required for normal hearing. May have a role in the development and maintenance of the stereocilia bundles. Might function as an anchoring/scaffolding protein in hair cells. Could be involved in the functional network formed by USH1C, CDH23 and MYO7A that is required for cohesion of the growing hair bundle Defects in USH1G are the cause of Usher syndrome type 1G (USH1G) [MIM:606943]. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness B0ABB9F5711A5095 ush1c-ush1g-4 2 8 unspecified role unspecified role unspecified role unspecified role SAM-PAM domain Region 384-461 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role N-terminal and PDZ1 domains Region 1-192 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ush1c-ush1g-5 6 8 unspecified role unspecified role prey prey SAM domain Region 384-458 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait N-terminal and PDZ1 domains Region 1-192 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ush1g-1 5 8 unspecified role unspecified role unspecified role unspecified role SAM-PAM domain Region 384-461 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q495M9:p.Lys437Glu K437E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K437E'. Wed May 02 15:37:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys437glu' Tue Jun 30 18:03:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys437Glu' ecolx Escherichia coli direct interaction direct interaction ush1g-2 4 8 unspecified role unspecified role unspecified role unspecified role SAM-PAM domain Region 384-461 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q495M9:p.Lys437Glu K437E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K437E'. Wed May 02 14:26:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys437glu' Tue Jun 30 16:38:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys437Glu' ecolx Escherichia coli direct interaction direct interaction ush1c-ush1g-6 1 8 unspecified role unspecified role unspecified role unspecified role SAM-PAM domain Region 384-461 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q495M9:p.Asp458Val D458V mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D V This feature has been corrected as a result of our quality control procedures. The original label was 'D458V'. Wed May 02 20:05:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp458val' Tue Jun 30 18:14:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp458Val' ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role N-terminal and PDZ1 domains Region 1-192 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9Y6N9:p.Arg103His R103H mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H This feature has been corrected as a result of our quality control procedures. The original label was 'R103H'. Wed May 02 16:58:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg103his' Tue Jun 30 20:09:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg103His' ecolx Escherichia coli direct interaction direct interaction ush1c-ush1g-7 3 8 unspecified role unspecified role unspecified role unspecified role SAM-PAM domain Region 384-461 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role N-terminal and PDZ1 domains Region 1-192 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction