DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nature structural & molecular biology 2010 imex curation Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. DIP original experiment: DIP-133979X Nature structural & molecular biology 2010 IMEx Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nature structural & molecular biology 2010 imex curation Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. DIP original experiment: DIP-133981X Nature structural & molecular biology 2010 IMEx Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nature structural & molecular biology 2010 imex curation Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. DIP original experiment: DIP-133980X Nature structural & molecular biology 2010 IMEx Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nature structural & molecular biology 2010 imex curation Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. DIP original experiment: DIP-133977X Nature structural & molecular biology 2010 IMEx Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. Nature structural & molecular biology 2010 imex curation Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Jinek M., Fabian MR., Coyle SM., Sonenberg N., Doudna JA. DIP original experiment: DIP-133978X Nature structural & molecular biology 2010 IMEx Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. tnr6c_human Trinucleotide repeat-containing gene 6C protein TNRC6C KIAA1582 protein protein human Homo sapiens Human MATGSAQGNFTGHTKKTNGNNGTNGALVQSPSNQSALGAGGANSNGSAARVWGVATGSSSGLAHCSVSGGDGKMDTMIGDGRSQNCWGASNSNAGINLNLNPNANPAAWPVLGHEGTVATGNPSSICSPVSAIGQNMGNQNGNPTGTLGAWGNLLPQESTEPQTSTSQNVSFSAQPQNLNTDGPNNTNPMNSSPNPINAMQTNGLPNWGMAVGMGAIIPPHLQGLPGANGSSVSQVSGGSAEGISNSVWGLSPGNPATGNSNSGFSQGNGDTVNSALSAKQNGSSSAVQKEGSGGNAWDSGPPAGPGILAWGRGSGNNGVGNIHSGAWGHPSRSTSNGVNGEWGKPPNQHSNSDINGKGSTGWESPSVTSQNPTVQPGGEHMNSWAKAASSGTTASEGSSDGSGNHNEGSTGREGTGEGRRRDKGIIDQGHIQLPRNDLDPRVLSNTGWGQTPVKQNTAWEFEESPRSERKNDNGTEAWGCAATQASNSGGKNDGSIMNSTNTSSVSGWVNAPPAAVPANTGWGDSNNKAPSGPGVWGDSISSTAVSTAAAAKSGHAWSGAANQEDKSPTWGEPPKPKSQHWGDGQRSNPAWSAGGGDWADSSSVLGHLGDGKKNGSGWDADSNRSGSGWNDTTRSGNSGWGNSTNTKANPGTNWGETLKPGPQQNWASKPQDNNVSNWGGAASVKQTGTGWIGGPVPVKQKDSSEATGWEEPSPPSIRRKMEIDDGTSAWGDPSNYNNKTVNMWDRNNPVIQSSTTTNTTTTTTTTTSNTTHRVETPPPHQAGTQLNRSPLLGPGRKVSSGWGEMPNVHSKTENSWGEPSSPSTLVDNGTAAWGKPPSSGSGWGDHPAEPPVAFGRAGAPVAASALCKPASKSMQEGWGSGGDEMNLSTSQWEDEEGDVWNNAASQESTSSCSSWGNAPKKGLQKGMKTSGKQDEAWIMSRLIKQLTDMGFPREPAEEALKSNNMNLDQAMSALLEKKVDVDKRGLGVTDHNGMAAKPLGCRPPISKESSVDRPTFLDKDGGLVEEPTPSPFLPSPSLKLPLSHSALPSQALGGIASGLGMQNLNSSRQIPSGNLGMFGNSGAAQARTMQQPPQPPVQPLNSSQPSLRAQVPQFLSPQVQAQLLQFAAKNIGLNPALLTSPINPQHMTMLNQLYQLQLAYQRLQIQQQMLQAQRNVSGSMRQQEQQVARTITNLQQQIQQHQRQLAQALLVKQPPPPPPPPHLSLHPSAGKSAMDSFPSHPQTPGLPDLQTKEQQSSPNTFAPYPLAGLNPNMNVNSMDMTGGLSVKDPSQSQSRLPQWTHPNSMDNLPSAASPLEQNPSKHGAIPGGLSIGPPGKSSIDDSYGRYDLIQNSESPASPPVAVPHSWSRAKSDSDKISNGSSINWPPEFHPGVPWKGLQNIDPENDPDVTPGSVPTGPTINTTIQDVNRYLLKSGGKLSDIKSTWSSGPTSHTQASLSHELWKVPRNSTAPTRPPPGLTNPKPSSTWGASPLGWTSSYSSGSAWSTDTSGRTSSWLVLRNLTPQIDGSTLRTLCLQHGPLITFHLNLTQGNAVVRYSSKEEAAKAQKSLHMCVLGNTTILAEFAGEEEVNRFLAQGQALPPTSSWQSSSASSQPRLSAAGSSHGLVRSDAGHWNAPCLGGKGSSELLWGGVPQYSSSLWGPPSADDSRVIGSPTPLTTLLPGDLLSGESL DIP protein Q9HCJ0 original sequence version: 57 DIP protein Q9HCJ0-1 original sequence version: 56 DIP protein Q9HCJ0-1 original sequence version: 68 C8230D65BFB1DA0B pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV DIP protein P11940 original sequence version: 187 DIP protein P11940 original sequence version: 181 DIP protein P11940-1 original sequence version: 136 DIP protein P11940 original sequence version: 148 DIP protein P11940 original sequence version: 172 2EB1B02A346132EE pabpc1-tnrc6c-5 4 7 unspecified role unspecified role unspecified role unspecified role PABC domain Region 545-619 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role DUF domain peptide Region 1382-1399 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction tnrc6c-pabpc1-1 1 7 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait Q9HCJ0:p.[Glu1388Ala;Phe1389Ala] E1388A/F1389A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E1388A/F1389A'. Wed May 02 19:02:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1388ala,phe1389ala' Tue Jun 30 15:50:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu1388Ala;Phe1389Ala]' Q9HCJ0:p.[Trp1395Ala;Lys1396Ala] W1395A/K1396A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W1395A/K1396A'. Wed May 02 16:54:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp1395ala,lys1396ala' Tue Jun 30 19:45:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp1395Ala;Lys1396Ala]' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range DUF domain peptide Region 1382-1399 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction pabpc1-tnrc6c-6 2 7 unspecified role unspecified role unspecified role unspecified role PABC domain Region 545-619 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role DUF domain peptide Region 1382-1399 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction pabpc1-tnrc6c-7 5 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range DUF-RRM domain Region 1382-1690 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9HCJ0:p.[Glu1388Ala;Phe1389Ala;Trp1395Ala;Lys1396Ala] E1388A/F1389A/W1395A/K1396A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E1388A/F1389A/W1395A/K1396A'. Wed May 02 16:29:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1388ala,phe1389ala,trp1395ala,lys1396ala' Tue Jun 30 16:53:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu1388Ala;Phe1389Ala;Trp1395Ala;Lys1396Ala]' human Homo sapiens Human physical association physical association tnrc6c-pabpc1-2 3 7 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range DUF-RRM domain Region 1364-1601 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction