DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219195X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219196X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219197X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219194X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219193X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Ras membrane orientation and nanodomain localization generate isoform diversity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Abankwa D., Gorfe AA., Inder K., Hancock JF. Only protein-protein interactions crisp Cricetidae sp. Hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Abankwa D., Gorfe AA., Inder K., Hancock JF. DIP original experiment: DIP-219192X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Ras membrane orientation and nanodomain localization generate isoform diversity. rasm_human Ras-related protein M-Ras MRAS RRAS3 Ras-related protein R-Ras3 protein protein human Homo sapiens Human MATSAVPSDNLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKITREQGKEMATKHNIPYIETSAKDPPLNVDKAFHDLVRVIRQQIPEKSQKKKKKTKWRGDRATGTHKLQCVIL DIP protein O14807 original sequence version: 107 0B2B55AFA96B3EC4 leg1_rat Galectin-1 Lectin galactoside-binding soluble 1 Beta-galactoside-binding lectin L-14-I S-Lac lectin 1 Galaptin 14 kDa lectin RL 14.5 Lgals1 Lactose-binding lectin 1 protein protein rat Rattus norvegicus (Rat) Rat MACGLVASNLNLKPGECLKVRGELAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDDGTWGTEQRETAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE DIP protein P11762 original sequence version: 90 A2C2DFB464FE81CF rasn_human GTPase NRas Transforming protein N-Ras NRAS HRAS1 protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM DIP protein P01111 original sequence version: 105 DIP protein P01111 original sequence version: 131 DIP protein P01111 original sequence version: 135 DIP protein P01111 original sequence version: 138 6898D3F6815B1EC7 pk3ca_bovin Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Phosphoinositide-3-kinase catalytic alpha polypeptide Serine/threonine protein kinase PIK3CA PIK3CA Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha protein protein bovin Bos taurus (Bovine) Bovine MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLRAICTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN DIP protein P32871 original sequence version: 93 C753DCC2F39FDDF0 p01116-2 GTPase KRas KRAS KRAS2 RASK2 K-Ras 2 Ki-Ras c-K-ras c-Ki-ras K-Ras4B protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM DIP protein P01116-2 original sequence version: 184 DIP protein P01116-2 original sequence version: 140 DIP protein P01116-2 original sequence version: 190 DIP protein P01116-2 original sequence version: 165 Variant in position: 152:V->G (in NS3). Variant in position: 153:D->V (in CFC2 and NS3, exhibits only minor alterations in its in vitro biochemical behavior compared to wild-type protein). Variant in position: 156:F->I (in NS3/CFC2). Variant in position: 156:F->L (found in a patient with Costello syndrome, exhibits an increase in intrinsic and guanine nucleotide exchange factor catalyzed nucleotide exchange in combination with an impaired GTPase-activating protein-stimulated GTP hydrolysis but functional in interaction with effectors). B1B6D189BB259861 raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS DIP protein P01112 original sequence version: 91 DIP protein P01112 original sequence version: 106 DIP protein P01112 original sequence version: 144 DIP protein P01112 original sequence version: 118 DIP protein P01112 original sequence version: 143 DIP protein P01112 original sequence version: 152 DIP protein P01112-1 original sequence version: 159 DIP protein P01112 original sequence version: 170 DIP protein P01112-1 original sequence version: 185 DIP protein P01112 original sequence version: 192 DIP protein P01112-1 original sequence version: 196 DIP protein P01112-1 original sequence version: 202 EE6DC2D933E2856A hras-pik3ca-3 6 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD domain Region 173-292 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster 13 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P01112:p.[Arg169Ala;Lys170Ala] R169A/K170A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R169A/K170A'. Wed May 02 17:15:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg169ala,lys170ala' Tue Jun 30 16:16:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg169Ala;Lys170Ala]' crisp Cricetidae sp. Hamster direct interaction direct interaction kras-raf1-3 3 11 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01116-2:p.[Lys128Ala;Arg135Ala] K128A/R135A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K128A/R135A'. Wed May 02 20:30:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys128ala,arg135ala' Tue Jun 30 19:37:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys128Ala;Arg135Ala]' crisp Cricetidae sp. Hamster 12 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster direct interaction direct interaction mras-raf1-1 4 7 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster 12 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster direct interaction direct interaction hras-raf1-7 2 12 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster 13 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster direct interaction direct interaction hras-lgals1-1 5 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster 13 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P01112:p.[Arg169Ala;Lys170Ala] R169A/K170A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R169A/K170A'. Wed May 02 15:54:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg169ala,lys170ala' Tue Jun 30 20:14:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg169Ala;Lys170Ala]' crisp Cricetidae sp. Hamster direct interaction direct interaction nras-raf1-1 1 9 unspecified role unspecified role fluorescence donor fluorescence donor GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P01111:p.[Lys128Ala;Lys135Ala] K128A/K135A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K128A/K135A'. Wed May 02 16:46:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys128ala,lys135ala' Tue Jun 30 20:05:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys128Ala;Lys135Ala]' P01111:p.[Lys169Ala;Lys170Ala] K169A/K170A mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K169A/K170A'. Wed May 02 18:46:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys169ala,lys170ala' Tue Jun 30 18:38:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys169Ala;Lys170Ala]' crisp Cricetidae sp. Hamster 12 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept RBD-CRD domain Region 51-200 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crisp Cricetidae sp. Hamster direct interaction direct interaction