DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254084X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254085X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254092X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254087X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254083X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254086X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254081X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254089X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254088X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254091X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254082X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254090X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. eed_human Polycomb protein EED EED WD protein associating with integrin cytoplasmic tails 1 Embryonic ectoderm development protein protein protein human Homo sapiens Human MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR DIP protein O75530 original sequence version: 70 DIP protein O75530-1 original sequence version: 78 DIP protein O75530 original sequence version: 78 DIP protein O75530 original sequence version: 73 DIP protein O75530-1 original sequence version: 89 DIP protein O75530-1 original sequence version: 87 DIP protein O75530 original sequence version: 77 D2E0A5BA27C0499A tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR DIP protein P19438 original sequence version: 135 DIP protein P19438 original sequence version: 150 DIP protein P19438 original sequence version: 177 4CEFBA96D03B8225 nsma2_mouse Sphingomyelin phosphodiesterase 3 Smpd3 Neutral sphingomyelinase 2 Neutral sphingomyelinase II protein protein mouse Mus musculus Mouse MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLLASFIPTTYEKRQRADDPCCLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKNPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGSRAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRPQEADDPAAGSQARNGAGGTPKGQTPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGANSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAVRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDTNGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSAGEEEA DIP protein Q9JJY3 original sequence version: 72 6764769EEAB5A168 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 nsma2_human Sphingomyelin phosphodiesterase 3 Neutral sphingomyelinase II Neutral sphingomyelinase 2 SMPD3 protein protein human Homo sapiens Human MVLYTTPFPNSCLSALHCVSWALIFPCYWLVDRLAASFIPTTYEKRQRADDPCCLQLLCTALFTPIYLALLVASLPFAFLGFLFWSPLQSARRPYIYSRLEDKGLAGGAALLSEWKGTGPGKSFCFATANVCLLPDSLARVNNLFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGVARAVPGSIKRTASVEYKGDGGRHPGDEAANGPASGDPVDSSSPEDACIVRIGGEEGGRPPEADDPVPGGQARNGAGGGPRGQTPNHNQQDGDSGSLGSPSASRESLVKGRAGPDTSASGEPGANSKLLYKASVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAATKLKEQLHGYFEYILYDVGVYGCQGCCSFKCLNSGLLFASRYPIMDVAYHCYPNKCNDDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPQEDSAIRCGQLDLLQDWLADFRKSTSSSSAANPEELVAFDVVCGDFNFDNCSSDDKLEQQHSLFTHYRDPCRLGPGEEKPWAIGTLLDTNGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSSGQKGRKELLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSSGEEEA DIP protein Q9NY59 original sequence version: 65 C06401571633C48E tnfa_human Tumor necrosis factor TNF TNFA TNFSF2 TNF-alpha Tumor necrosis factor ligand superfamily member 2 Cachectin protein protein human Homo sapiens Human MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL DIP protein P01375 original sequence version: 198 DIP protein P01375 original sequence version: 151 DIP protein P01375 original sequence version: 156 DIP protein P01375 original sequence version: 186 DIP protein P01375 original sequence version: 201 DIP protein P01375 original sequence version: 174 DIP protein P01375 original sequence version: 211 3DF90F96C9031FFE smpd3-eed-1 7 13 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 15 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction smpd3-eed-2 5 13 unspecified role unspecified role prey prey human Homo sapiens Human 17 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association eed-smpd3-1 4 13 unspecified role unspecified role bait bait WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 15 unspecified role unspecified role prey prey C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction eed-rack1-1 8 16 unspecified role unspecified role prey prey WD repeat V-VII domain Region 204-317 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role bait bait WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction eed-smpd3-2 11 15 unspecified role unspecified role prey prey C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association smpd3-rack1-1 1 16 unspecified role unspecified role prey prey human Homo sapiens Human 17 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association eed-smpd3-3 10 13 unspecified role unspecified role bait bait human Homo sapiens Human 17 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association rack1-eed-1 9 16 unspecified role unspecified role bait bait WD repeat IV-VII domain Region 144-317 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role prey prey WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction smpd3-eed-3 6 13 unspecified role unspecified role prey prey WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 15 unspecified role unspecified role bait bait C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction tnf-eed-1 2 18 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 16 unspecified role unspecified role prey prey human Homo sapiens Human 17 unspecified role unspecified role prey prey human Homo sapiens Human 14 unspecified role unspecified role prey prey human Homo sapiens Human 13 unspecified role unspecified role prey prey human Homo sapiens Human association association smpd3-eed-4 12 15 unspecified role unspecified role bait bait C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association eed-rack1-2 3 13 unspecified role unspecified role bait bait human Homo sapiens Human 16 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association