DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu STIM1 gates the store-operated calcium channel ORAI1 in vitro. STIM1 gates the store-operated calcium channel ORAI1 in vitro. Nature structural & molecular biology 2010 imex curation Zhou Y., Meraner P., Kwon HT., Machnes D., Oh-hora M., Zimmer J., Huang Y., Stura A., Rao A., Hogan PG. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhou Y., Meraner P., Kwon HT., Machnes D., Oh-hora M., Zimmer J., Huang Y., Stura A., Rao A., Hogan PG. DIP original experiment: DIP-133972X Nature structural & molecular biology 2010 IMEx STIM1 gates the store-operated calcium channel ORAI1 in vitro. STIM1 gates the store-operated calcium channel ORAI1 in vitro. STIM1 gates the store-operated calcium channel ORAI1 in vitro. Nature structural & molecular biology 2010 imex curation Zhou Y., Meraner P., Kwon HT., Machnes D., Oh-hora M., Zimmer J., Huang Y., Stura A., Rao A., Hogan PG. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhou Y., Meraner P., Kwon HT., Machnes D., Oh-hora M., Zimmer J., Huang Y., Stura A., Rao A., Hogan PG. DIP original experiment: DIP-133970X Nature structural & molecular biology 2010 IMEx STIM1 gates the store-operated calcium channel ORAI1 in vitro. STIM1 gates the store-operated calcium channel ORAI1 in vitro. STIM1 gates the store-operated calcium channel ORAI1 in vitro. Nature structural & molecular biology 2010 imex curation Zhou Y., Meraner P., Kwon HT., Machnes D., Oh-hora M., Zimmer J., Huang Y., Stura A., Rao A., Hogan PG. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Zhou Y., Meraner P., Kwon HT., Machnes D., Oh-hora M., Zimmer J., Huang Y., Stura A., Rao A., Hogan PG. DIP original experiment: DIP-133971X Nature structural & molecular biology 2010 IMEx STIM1 gates the store-operated calcium channel ORAI1 in vitro. stim1_mouse Stromal interaction molecule 1 Stim1 Sim protein protein mouse Mus musculus Mouse MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMGRAADEALNAMPSNGSHRLIEGVHPGSLVEKLPDSPALAKKTFMALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPVGDNRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK DIP protein P70302 original sequence version: 84 mint Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1 F4DADF67436790FD crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA DIP protein Q96D31 original sequence version: 95 DIP protein Q96D31 original sequence version: 38 DIP protein Q96D31 original sequence version: 58 DIP protein Q96D31 original sequence version: 55 DIP protein Q96D31 original sequence version: 57 DIP protein Q96D31 original sequence version: 53 DIP protein Q96D31 original sequence version: 63 DIP protein Q96D31 original sequence version: 76 DIP protein Q96D31 original sequence version: 79 DIP protein Q96D31 original sequence version: 56 F85F7C06F36628D4 stim1-2 2 4 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 233-463 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction stim1-3 3 4 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 233-463 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction orai1-stim1-14 1 4 unspecified role unspecified role prey prey Cytoplasmic domain Region 233-498 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role bait bait Cytoplasmic tail Region 65-87 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction