DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215892X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215888X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215884X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215891X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm dls dynamic light scattering dls predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215885X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215883X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215886X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215887X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215890X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215889X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. scar Ligand peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SCARFFGTPGCT sigk Ligand peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SIGKAFKILGYPDYD phos_rat Phosducin Pdc Rpr1 33 kDa phototransducing protein Rod photoreceptor 1 Protein MEKA protein protein rat Rattus norvegicus (Rat) Rat MEEAASQSLEEDFEGQATHTGPKGVINDWRKFKLESEDGDSIPPSKKEILRQMSSPQSRDDKDSKERMSRKMSIQEYELIHQDKEDEGCLRKYRRQCMQDMHQKLSFGPRYGFVYELETGEQFLETIEKEQKVTTIVVNIYEDGVRGCDALNSSLECLAAEYPMVKFCKIRASNTGAGDRFSSDVLPTLLVYKGGELISNFISVAEQFAEDFFAADVESFLNEYGLLPEREIHDLGQTNTEDEDIE DIP protein P20942 original sequence version: 90 mint May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism 3C48ABCB4E5E3D04 gbg2_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 GNG2 G gamma-I protein protein bovin Bos taurus (Bovine) Bovine MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL DIP protein P63212 original sequence version: 54 DIP protein P63212 original sequence version: 55 DIP protein P63212 original sequence version: 66 EDB74E4135E7A37A gnai1_bovin Guanine nucleotide-binding protein G(i) subunit alpha-1 GNAI1 Adenylate cyclase-inhibiting G alpha protein protein protein bovin Bos taurus (Bovine) Bovine MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF DIP protein P63097 original sequence version: 52 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli mint 9F88311B46E62DE3 gbb1_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 GNB1 Transducin beta chain 1 protein protein bovin Bos taurus (Bovine) Bovine MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN DIP protein P62871 original sequence version: 29 DIP protein P62871 original sequence version: 61 DIP protein P62871 original sequence version: 64 896CBD32D2686598 gng2-gnb1-1 6 16 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role bait bait Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association gnb1-gng2-3 5 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction gnai1-gnb1-2 8 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association gnai1-gnb1-3 7 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association gnb1-scar-1 9 11 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction gnb1-pdc-1 1 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-107 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain C-terminal domain Region 108-246 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction gnb1-gng2-4 4 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction gnb1-gng2-5 3 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction gnai1-gnb1-4 2 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction gnb1-sigk-1 10 12 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction