DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology 2010 imex curation Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. DIP original experiment: DIP-133955X Nature structural & molecular biology 2010 IMEx Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology 2010 imex curation Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. DIP original experiment: DIP-133957X Nature structural & molecular biology 2010 IMEx Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology 2010 imex curation Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. Only protein-protein interactions ecolx Escherichia coli ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. DIP original experiment: DIP-133958X Nature structural & molecular biology 2010 IMEx Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology 2010 imex curation Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. DIP original experiment: DIP-133953X Nature structural & molecular biology 2010 IMEx Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology 2010 imex curation Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. DIP original experiment: DIP-133954X Nature structural & molecular biology 2010 IMEx Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology 2010 imex curation Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Nesić D., Miller MC., Quinkert ZT., Stein M., Chait BT., Stebbins CE. DIP original experiment: DIP-133956X Nature structural & molecular biology 2010 IMEx Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. caga_helpy Cytotoxicity-associated immunodominant antigen 120 kDa protein CAG pathogenicity island protein 26 cai HP_0547 cagA cag26 protein protein helpy Helicobacter pylori Campylobacter pylori MTNETIDQTRTPDQTQSQTAFDPQQFINNLQVAFIKVDNVVASFDPDQKPIVDKNDRDNRQAFDGISQLREEYSNKAIKNPTKKNQYFSDFIDKSNDLINKDNLIDVESSTKSFQKFGDQRYQIFTSWVSHQKDPSKINTRSIRNFMENIIQPPIPDDKEKAEFLKSAKQSFAGIIIGNQIRTDQKFMGVFDESLKERQEAEKNGGPTGGDWLDIFLSFIFNKKQSSDVKEAINQEPVPHVQPDIATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKFNQLLIHNNALSSVLMGSHNGIEPEKVSLLYAGNGGFGDKHDWNATVGYKDQQGNNVATLINVHMKNGSGLVIAGGEKGINNPSFYLYKEDQLTGSQRALSQEEIRNKVDFMEFLAQNNTKLDNLSEKEKEKFQNEIEDFQKDSKAYLDALGNDRIAFVSKKDTKHSALITEFNNGDLSYTLKDYGKKADKALDREKNVTLQGSLKHDGVMFVDYSNFKYTNASKNPNKGVGATNGVSHLEAGFNKVAVFNLPDLNNLAITSFVRRNLENKLTAKGLSLQEANKLIKDFLSSNKELAGKALNFNKAVAEAKSTGNYDEVKKAQKDLEKSLRKREHLEKEVEKKLESKSGNKNKMEAKAQANSQKDEIFALINKEANRDARAIAYTQNLKGIKRELSDKLEKISKDLKDFSKSFDEFKNGKNKDFSKAEETLKALKGSVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSDLENSVKDVIINQKVTDKVDNLNQAVSVAKAMGDFSRVEQVLADLKNFSKEQLAQQAQKNEDFNTGKNSELYQSVKNSVNKTLVGNGLSGIEATALAKNFSDIKKELNEKFKNFNNNNNGLKNSTEPIYAKVNKKKTGQVASPEEPIYTQVAKKVNAKIDRLNQIASGLGGVGQAAGFPLKRHDKVDDLSKVGLSASPEPIYATIDDLGGPFPLKRHDKVDDLSKVGRSRNQELAQKIDNLNQAVSEAKAGFFGNLEQTIDKLKDSTKKNVMNLYVESAKKVPASLSAKLDNYAINSHTRINSNIQNGAINEKATGMLTQKNPEWLKLVNDKIVAHNVGSVSLSEYDKIGFNQKNMKDYSDSFKFSTKLNNAVKDIKSGFTHFLANAFSTGYYCLARENAEHGIKNVNTKGGFQKS DIP protein P55980 original sequence version: 51 DIP protein P55980 original sequence version: 69 B05C3F2CCC4444F4 taok1_human Serine/threonine-protein kinase TAO1 Prostate-derived sterile 20-like kinase 2 Thousand and one amino acid protein kinase 1 TAOK1 KIAA1361 MAP3K16 MARKK MARK Kinase Kinase from chicken homolog B protein protein human Homo sapiens Human MPSTNRAGSLKDPEIAELFFKEDPEKLFTDLREIGHGSFGAVYFARDVRTNEVVAIKKMSYSGKQSTEKWQDIIKEVKFLQRIKHPNSIEYKGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALQGLAYLHSHTMIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWSLGITCIELAERKPPLFNMNAMSALYHIAQNESPTLQSNEWSDYFRNFVDSCLQKIPQDRPTSEELLKHIFVLRERPETVLIDLIQRTKDAVRELDNLQYRKMKKLLFQEAHNGPAVEAQEEEEEQDHGVGRTGTVNSVGSNQSIPSMSISASSQSSSVNSLPDVSDDKSELDMMEGDHTVMSNSSVIHLKPEEENYREEGDPRTRASDPQSPPQVSRHKSHYRNREHFATIRTASLVTRQMQEHEQDSELREQMSGYKRMRRQHQKQLMTLENKLKAEMDEHRLRLDKDLETQRNNFAAEMEKLIKKHQAAMEKEAKVMSNEEKKFQQHIQAQQKKELNSFLESQKREYKLRKEQLKEELNENQSTPKKEKQEWLSKQKENIQHFQAEEEANLLRRQRQYLELECRRFKRRMLLGRHNLEQDLVREELNKRQTQKDLEHAMLLRQHESMQELEFRHLNTIQKMRCELIRLQHQTELTNQLEYNKRRERELRRKHVMEVRQQPKSLKSKELQIKKQFQDTCKIQTRQYKALRNHLLETTPKSEHKAVLKRLKEEQTRKLAILAEQYDHSINEMLSTQALRLDEAQEAECQVLKMQLQQELELLNAYQSKIKMQAEAQHDRELRELEQRVSLRRALLEQKIEEEMLALQNERTERIRSLLERQAREIEAFDSESMRLGFSNMVLSNLSPEAFSHSYPGASGWSHNPTGGPGPHWGHPMGGPPQAWGHPMQGGPQPWGHPSGPMQGVPRGSSMGVRNSPQALRRTASGGRTEQGMSRSTSVTSQISNGSHMSYT DIP protein Q7L7X3 original sequence version: 62 82941DEAEADC7651 mark2_human Serine/threonine-protein kinase MARK2 MAP/microtubule affinity-regulating kinase 2 ELKL motif kinase 1 PAR1 homolog MARK2 EMK1 PAR1 homolog b protein protein human Homo sapiens Human MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL DIP protein Q7KZI7 original sequence version: 53 DIP protein Q7KZI7 original sequence version: 76 DIP protein Q7KZI7 original sequence version: 70 F307BF4DDB543A70 tr1tau TR1 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KNVKSKIGSTENLK caga-mark2-1 4 7 unspecified role unspecified role bait bait EPIYA repeat domain Region 885-981 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P55980:p.Leu950Gly L950G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L G This feature has been corrected as a result of our quality control procedures. The original label was 'L950G'. Wed May 02 19:32:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu950gly' Tue Jun 30 18:41:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu950Gly' P55980:p.Arg952Gly R952G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G This feature has been corrected as a result of our quality control procedures. The original label was 'R952G'. Wed May 02 15:23:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg952gly' Tue Jun 30 18:22:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg952Gly' P55980:p.Val956Gly V956G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V G This feature has been corrected as a result of our quality control procedures. The original label was 'V956G'. Wed May 02 14:59:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val956gly' Tue Jun 30 15:55:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val956Gly' P55980:p.Leu959Gly L959G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L G This feature has been corrected as a result of our quality control procedures. The original label was 'L959G'. Wed May 02 16:57:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu959gly' Tue Jun 30 18:26:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu959Gly' ecolx Escherichia coli 9 unspecified role unspecified role prey prey Kinase domain Region 39-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7KZI7:p.[Glu136Gly;Phe138Gly;Asp139Gly] E136G/F138G/D139G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D G certain certain sequence position certain certain certain sequence position certain F G certain certain sequence position certain certain certain sequence position certain E G This feature has been corrected as a result of our quality control procedures. The original label was 'E136G/F138G/D139G'. Wed May 02 18:45:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu136gly,phe138gly,asp139gly' Tue Jun 30 15:53:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu136Gly;Phe138Gly;Asp139Gly]' ecolx Escherichia coli physical association physical association mark2-taok1-1 2 8 enzyme enzyme unspecified role unspecified role Region 1-314 Region 1-314 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 enzyme target enzyme target substrate substrate unspecified role unspecified role Kinase domain Region 39-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation caga-mark2-2 1 7 unspecified role unspecified role unspecified role unspecified role EPIYA repeat domain Region 885-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Kinase domain Region 39-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction caga-mark2-3 3 7 unspecified role unspecified role unspecified role unspecified role EPIYA repeat domain Region 885-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Kinase domain Region 39-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mark2-tr1tau-1 6 10 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro 9 enzyme enzyme unspecified role unspecified role Kinase domain Region 39-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation caga-mark2-4 5 7 unspecified role unspecified role unspecified role unspecified role EPIYA repeat domain Region 885-981 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Kinase domain Region 39-364 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction