DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Wang ZA., Kalderon D. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang ZA., Kalderon D. DIP original experiment: DIP-134870X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. p54733-2 G1/S-specific cyclin-E B CycE CG3938 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKLEQKRKFIEMDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP DIP protein P54733-2 original sequence version: 88 0EF379437F9A3ED6 cdk2_drome Cyclin-dependent kinase 2 Cdk2 Cell division control protein 2 cognate cdc2c CG10498 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV DIP protein P23573 original sequence version: 103 576A88767F9D35C0 cyce-cdk2-1 1 3 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 2 unspecified role unspecified role bait bait lys_val_glu_glu_ile_tyr_pro311-317ala_ala_ala_ala_ala_ala_ala K311A/V312A/E313A/E314A/I315A/Y316A/P317A required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain KVEEIYP AAAAAAA Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range This tag was used for western blot detection. drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association