DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Nature 2009 imex curation Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. DIP original experiment: DIP-254226X Nature 2009 IMEx Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Nature 2009 imex curation Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. DIP original experiment: DIP-254223X Nature 2009 IMEx Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Nature 2009 imex curation Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. DIP original experiment: DIP-254225X Nature 2009 IMEx Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Nature 2009 imex curation Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. DIP original experiment: DIP-254227X Nature 2009 IMEx Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Direct inhibition of the NOTCH transcription factor complex. Nature 2009 imex curation Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Moellering RE., Cornejo M., Davis TN., Del Bianco C., Aster JC., Blacklow SC., Kung AL., Gilliland DG., Verdine GL., Bradner JE. DIP original experiment: DIP-254224X Nature 2009 IMEx Direct inhibition of the NOTCH transcription factor complex. q06330-6 Recombining binding protein suppressor of hairless RBPJ IGKJRB IGKJRB1 RBPJK RBPSUH CBF-1 J kappa-recombination signal-binding protein RBP-J kappa Renal carcinoma antigen NY-REN-30 protein protein human Homo sapiens Human MAWIKRKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS DIP protein Q06330-6 original sequence version: 98 DIP protein Q06330-6 original sequence version: 106 DIP protein Q06330-6 original sequence version: 118 31FD3B3D01B13C4C notc1_human Neurogenic locus notch homolog protein 1 Translocation-associated notch protein TAN-1 NOTCH1 TAN1 protein protein human Homo sapiens Human MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK DIP protein P46531 original sequence version: 173 DIP protein P46531 original sequence version: 103 DIP protein P46531 original sequence version: 109 DIP protein P46531 original sequence version: 127 DIP protein P46531 original sequence version: 137 DIP protein P46531 original sequence version: 186 DIP protein P46531 original sequence version: 150 DIP protein P46531 original sequence version: 142 DIP protein P46531 original sequence version: 181 E173C872D195F028 maml1_human Mastermind-like protein 1 MAML1 KIAA0200 protein protein human Homo sapiens Human MVLPTCPMAEFALPRHSAVMERLRRRIELCRRHHSTCEARYEAVSPERLELERQHTFALHQRCIQAKAKRAGKHRQPPAATAPAPAAPAPRLDAADGPEHGRPATHLHDTVKRNLDSATSPQNGDQQNGYGDLFPGHKKTRREAPLGVAISSNGLPPASPLGQSDKPSGADALQSSGKHSLGLDSLNKKRLADSSLHLNGGSNPSESFPLSLNKELKQEPVEDLPCMITGTVGSISQSNLMPDLNLNEQEWKELIEELNRSVPDEDMKDLFNEDFEEKKDPESSGSATQTPLAQDINIKTEFSPAAFEQEQLGSPQVRAGSAGQTFLGPSSAPVSTDSPSLGGSQTLFHTSGQPRADNPSPNLMPASAQAQNAQRALAGVVLPSQGPGGASELSSAHQLQQIAAKQKREQMLQNPQQATPAPAPGQMSTWQQTGPSHSSLDVPYPMEKPASPSSYKQDFTNSKLLMMPSVNKSSPRPGGPYLQPSHVNLLSHQPPSNLNQNSANNQGSVLDYGNTKPLSHYKADCGQGSPGSGQSKPALMAYLPQQLSHISHEQNSLFLMKPKPGNMPFRSLVPPGQEQNPSSVPVQAQATSVGTQPPAVSVASSHNSSPYLSSQQQAAVMKQHQLLLDQQKQREQQQKHLQQQQFLQRQQHLLAEQEKQQFQRHLTRPPPQYQDPTQGSFPQQVGQFTGSSAAVPGMNTLGPSNSSCPRVFPQAGNLMPMGPGHASVSSLPTNSGQQDRGVAQFPGSQNMPQSSLYGMASGITQIVAQPPPQATNGHAHIPRQTNVGQNTSVSAAYGQNSLGSSGLSQQHNKGTLNPGLTKPPVPRVSPAMGGQNSSWQHQGMPNLSGQTPGNSNVSPFTAASSFHMQQQAHLKMSSPQFSQAVPNRPMAPMSSAAAVGSLLPPVSAQQRTSAPAPAPPPTAPQQGLPGLSPAGPELGAFSQSPASQMGGRAGLHCTQAYPVRTAGQELPFAYSGQPGGSGLSSVAGHTDLIDSLLKNRTSEEWMSDLDDLLGSQ DIP protein Q92585 original sequence version: 108 DIP protein Q92585 original sequence version: 52 DIP protein Q92585 original sequence version: 71 DIP protein Q92585 original sequence version: 79 DIP protein Q92585 original sequence version: 91 C683CB81B73A2A61 suh_human Recombining binding protein suppressor of hairless J kappa-recombination signal-binding protein RBP-J kappa CBF-1 Renal carcinoma antigen NY-REN-30 RBPJ IGKJRB IGKJRB1 RBPJK RBPSUH protein protein human Homo sapiens Human MDHTEGSPAEEPPAHAPSPGKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS DIP protein Q06330 original sequence version: 139 DIP protein Q06330 original sequence version: 98 DIP protein Q06330 original sequence version: 154 DIP protein Q06330-1 original sequence version: 154 91E50D2DE9087EDA notch1-maml1-2 2 8 unspecified role unspecified role prey prey Region 13-74 Region 13-74 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CSL-binding domain Region 1761-2127 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 9-435 Region 9-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association maml1-notch1-1 4 9 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait Region 21-36 Region 21-36 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role prey prey human Homo sapiens Human association association notch1-rbpj-4 3 7 unspecified role unspecified role bait bait CSL-binding domain Region 1761-2127 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 9-435 Region 9-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction notch1-rbpj-5 5 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CSL-binding domain Region 1761-2127 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 9-435 Region 9-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction notch1-maml1-3 1 8 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association