DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133301X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133305X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133306X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133304X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133303X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133300X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature structural & molecular biology 2009 imex curation Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Levin KB., Dym O., Albeck S., Magdassi S., Keeble AH., Kleanthous C., Tawfik DS. DIP original experiment: DIP-133302X Nature structural & molecular biology 2009 IMEx Following evolutionary paths to protein-protein interactions with high affinity and selectivity. imm9_ecolx Colicin-E9 immunity protein imm ceiE9 ImmE9 Microcin-E9 immunity protein protein protein ecolx Escherichia coli MELKHSISDYTEAEFLQLVTTICNADTSSEEELVKLVTHFEEMTEHPSGSDLIYYPKEGDDDSPSGIVNTVKQWRAANGKSGFKQG DIP protein P13479 original sequence version: 66 DIP protein P13479 original sequence version: 73 136CBD25D071FDB4 cea7_ecolx Colicin-E7 colE7 cea protein protein ecolx Escherichia coli MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNPWGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISVSGEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVNNKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK DIP protein Q47112 original sequence version: 89 DIP protein Q47112 original sequence version: 69 E5B05E73B2E17249 cea9_ecolx Colicin-E9 col cei protein protein ecolx Escherichia coli MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNPWGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNDSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEEVSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIRVTTPKRHIDIHRGK DIP protein P09883 original sequence version: 99 DIP protein P09883 original sequence version: 76 DIP protein P09883 original sequence version: 81 DIP protein P09883 original sequence version: 84 DIP protein P09883 original sequence version: 86 47A71B57B45AFDD9 imm7_ecolx Colicin-E7 immunity protein imm ceiE7 ImmE7 Microcin-E7 immunity protein protein protein ecolx Escherichia coli MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKITEHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG DIP protein Q03708 original sequence version: 77 DIP protein Q03708 original sequence version: 58 DIP protein Q03708 original sequence version: 82 2CA7D97057529335 cole7-imm-6 2 11 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction cole7-imm-7 6 8 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction cole7-imm-8 5 11 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction cole7-imm-9 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 446-576 Region 446-576 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction col-imm-2 3 10 unspecified role unspecified role bait bait ecolx Escherichia coli 11 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction col-imm-3 4 10 unspecified role unspecified role bait bait ecolx Escherichia coli 8 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction cole7-imm-10 7 8 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction