IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. The Journal of Biological Chemistry 2009 imex curation Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at Accepted 2017-NOV-08 AT 12:58 GMT AT 12:58 GMT by ORCHARD The Journal of Biological Chemistry 2009 imex curation moved Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. The Journal of Biological Chemistry 2009 imex curation Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at Accepted 2017-NOV-08 AT 12:58 GMT AT 12:58 GMT by ORCHARD The Journal of Biological Chemistry 2009 imex curation moved Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. The Journal of Biological Chemistry 2009 imex curation Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. Isolation of DRMs (Detergent Resistant Rafts). rainer.prohaska@meduniwien.ac.at Accepted 2017-NOV-08 AT 12:58 GMT AT 12:58 GMT by ORCHARD The Journal of Biological Chemistry 2009 imex curation moved Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. The Journal of Biological Chemistry 2009 imex curation Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. rainer.prohaska@meduniwien.ac.at 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mairhofer M., Steiner M., Salzer U., Prohaska R., Mairhofer M., Steiner M., Salzer U., Prohaska R. Results from figure 8 and Supp fig 4 have not been entered as artificial chimeric/fusion proteins were used which are of NO biological relevance. methods: under "Fluorescence Microscopy" -Ref 42 only mentions "confocal microscopy" so have entered as this here, despite not being explicitly mentioned for Fig 4. rainer.prohaska@meduniwien.ac.at Accepted 2017-NOV-08 AT 12:58 GMT AT 12:58 GMT by ORCHARD The Journal of Biological Chemistry 2009 imex curation moved stom_human Stomatin STOM BND7 EPB72 Protein 7.2b Erythrocyte band 7 integral membrane protein Erythrocyte membrane protein band 7.2 protein protein human Homo sapiens Human MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITESPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG 5FEDA92230D99A24 stml1_human Stomatin-like protein 1 SLP1 UNC24 EPB72-like protein 1 Protein unc-24 homolog Stomatin-related protein MSTP019 STOML1 protein protein human Homo sapiens Human MLGRSGYRALPLGDFDRFQQSSFGFLGSQKGCLSPERGGVGTGADVPQSWPSCLCHGLISFLGFLLLLVTFPISGWFALKIVPTYERMIVFRLGRIRTPQGPGMVLLLPFIDSFQRVDLRTRAFNVPPCKLASKDGAVLSVGADVQFRIWDPVLSVMTVKDLNTATRMTAQNAMTKALLKRPLREIQMEKLKISDQLLLEINDVTRAWGLEVDRVELAVEAVLQPPQDSPAGPNLDSTLQQLALHFLGGSMNSMAGGAPSPGPADTVEMVSEVEPPAPQVGARSSPKQPLAEGLLTALQPFLSEALVSQVGACYQFNVVLPSGTQSAYFLDLTTGRGRVGHGVPDGIPDVVVEMAEADLRALLCRELRPLGAYMSGRLKVKGDLAMAMKLEAVLRALK A9850C26394E1C3D stom-stoml1-1 4 5 unspecified role unspecified role neutral component neutral component fixed cell fixed cell naturally occurring naturally occurring endogenous endogenous 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 4A stom-stoml1-2 1 5 unspecified role unspecified role bait bait naturally occurring naturally occurring endogenous endogenous 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5A Stomatin co-precipitated with both SLP-1-GFP and SLP-1-(1–288)-GFP the band for the 1-224-GFP looks much stronger than the 1-288 band..-so have entered as such?OK (far RHS band) stom-stoml1-3 2 5 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5A-2 stom-stoml1-4 3 5 unspecified role unspecified role neutral component neutral component naturally occurring naturally occurring endogenous endogenous 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Figure six, Lanes 1+2 and 6-9, Supp fig 3 SLP-1-GFP was detected in DRM fractions 1–2 but also in high-density fractions representing solubilized protein. The distribution of stomatin was almost identical.