UniProt UniProt http://www.uniprot.org RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Molecular and cellular biochemistry 2009 imex curation Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Accepted 2017-FEB-06 AT 15:07 GMT AT 15:07 GMT by ORCHARD Molecular and cellular biochemistry 2009 imex curation RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Molecular and cellular biochemistry 2009 imex curation Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Accepted 2017-FEB-06 AT 15:07 GMT AT 15:07 GMT by ORCHARD Molecular and cellular biochemistry 2009 imex curation RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Molecular and cellular biochemistry 2009 imex curation Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Accepted 2017-FEB-06 AT 15:07 GMT AT 15:07 GMT by ORCHARD Molecular and cellular biochemistry 2009 imex curation RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Molecular and cellular biochemistry 2009 imex curation Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Accepted 2017-FEB-06 AT 15:07 GMT AT 15:07 GMT by ORCHARD Molecular and cellular biochemistry 2009 imex curation RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Molecular and cellular biochemistry 2009 imex curation Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shan J., Wang P., Zhou J., Wu D., Shi H., Huo K. Origin of transfected Flag-RIP1 and Flag-NIK (CoIP experiments fig. 4B and C) cannot be established; experiments omitted kkhuo@fudan.edu.cn Accepted 2017-FEB-06 AT 15:07 GMT AT 15:07 GMT by ORCHARD human fetal brain cDNA Molecular and cellular biochemistry 2009 imex curation caspa_human Caspase-10 ICE-like apoptotic protease 4 Apoptotic protease Mch-4 FAS-associated death domain protein interleukin-1B-converting enzyme 2 CASP10 MCH4 protein protein human Homo sapiens Human MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRMLKFLEKTMEIRGRKRTVWGAKQISATSLPTAISAQTPRPPMRRWSSVS 840348AE602B8243 riok3_human Serine/threonine-protein kinase RIO3 sudD homolog RIOK3 SUDD RIO kinase 3 protein protein human Homo sapiens Human MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE 3C3392BAC4ECB912 q92851-7 Caspase-10 Apoptotic protease Mch-4 FAS-associated death domain protein interleukin-1B-converting enzyme 2 ICE-like apoptotic protease 4 10-G 10g CASP10 MCH4 protein protein human Homo sapiens Human MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPEGVFVFLNEGDRGNSPDDL 52370B5AD12C4DCF riok3-casp10-1 5 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 7 unspecified role unspecified role bait bait physical association physical association no figure riok3-casp10-2 1 8 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3A casp10-riok3-1 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 3B casp10-riok3-2 3 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3C casp10-riok3-3 3 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3D riok3-1 4 7 putative self putative self putative self putative self O14730:p.Lys290Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:25:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys290ala' Tue Jun 30 16:49:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys290Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation true 5A