DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The glucanosyltransferase Gas1 functions in transcriptional silencing. The glucanosyltransferase Gas1 functions in transcriptional silencing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Koch MR., Pillus L. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Koch MR., Pillus L. DIP original experiment: DIP-134339X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The glucanosyltransferase Gas1 functions in transcriptional silencing. The glucanosyltransferase Gas1 functions in transcriptional silencing. The glucanosyltransferase Gas1 functions in transcriptional silencing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Koch MR., Pillus L. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Koch MR., Pillus L. DIP original experiment: DIP-134340X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The glucanosyltransferase Gas1 functions in transcriptional silencing. sir2_yeast NAD-dependent histone deacetylase SIR2 SIR2 MAR1 YDL042C D2714 Silent information regulator 2 Regulatory protein SIR2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTIPHMKYAVSKTSENKVSNTVSPTQDKDAIRKQPDDIINNDEPSHKKIKVAQPDSLRETNTTDPLGHTKAALGEVASMELKPTNDMDPLAVSAASVVSMSNDVLKPETPKGPIIISKNPSNGIFYGPSFTKRESLNARMFLKYYGAHKFLDTYLPEDLNSLYIYYLIKLLGFEVKDQALIGTINSIVHINSQERVQDLGSAISVTNVEDPLAKKQTVRLIKDLQRAINKVLCTRLRLSNFFTIDHFIQKLHTARKILVLTGAGVSTSLGIPDFRSSEGFYSKIKHLGLDDPQDVFNYNIFMHDPSVFYNIANMVLPPEKIYSPLHSFIKMLQMKGKLLRNYTQNIDNLESYAGISTDKLVQCHGSFATATCVTCHWNLPGERIFNKIRNLELPLCPYCYKKRREYFPEGYNNKVGVAASQGSMSERPPYILNSYGVLKPDITFFGEALPNKFHKSIREDILECDLLICIGTSLKVAPVSEIVNMVPSHVPQVLINRDPVKHAEFDLSLLGYCDDIAAMVAQKCGWTIPHKKWNDLKNKNFKCQEKDKGVYVVTSDEHPKTL 52E6937533654586 52E6937533654586 gas1_yeast 1,3-beta-glucanosyltransferase GAS1 GAS1 GGP1 YMR307W YM9952.09 Glycoprotein GP115 Glycolipid-anchored surface protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLFKSLSKLATAAAFFAGVATADDVPAIEVVGNKFFYSNNGSQFYIRGVAYQADTANETSGSTVNDPLANYESCSRDIPYLKKLNTNVIRVYAINTTLDHSECMKALNDADIYVIADLAAPATSINRDDPTWTVDLFNSYKTVVDTFANYTNVLGFFAGNEVTNNYTNTDASAFVKAAIRDVRQYISDKNYRKIPVGYSSNDDEDTRVKMTDYFACGDDDVKADFYGINMYEWCGKSDFKTSGYADRTAEFKNLSIPVFFSEYGCNEVTPRLFTEVEALYGSNMTDVWSGGIVYMYFEETNKYGLVSIDGNDVKTLDDFNNYSSEINKISPTSANTKSYSATTSDVACPATGKYWSAATELPPTPNGGLCSCMNAANSCVVSDDVDSDDYETLFNWICNEVDCSGISANGTAGKYGAYSFCTPKEQLSFVMNLYYEKSGGSKSDCSFSGSATLQTATTQASCSSALKEIGSMGTNSASGSVDLGSGTESSTASSNASGSSSKSNSGSSGSSSSSSSSSASSSSSSKKNAATNVKANLAQVVFTSIISLSIAAGVGFALV D6E39568DCB4C5AE D6E39568DCB4C5AE sir2-gas1-1 1 4 unspecified role unspecified role prey prey Region 163-407 Region 163-407 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 unspecified role unspecified role bait bait Region 244-457 Region 244-457 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association sir2-gas1-2 2 4 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association