DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nature structural & molecular biology 2009 imex curation Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. DIP original experiment: DIP-133041X Nature structural & molecular biology 2009 IMEx Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nature structural & molecular biology 2009 imex curation Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. DIP original experiment: DIP-133043X Nature structural & molecular biology 2009 IMEx Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nature structural & molecular biology 2009 imex curation Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. DIP original experiment: DIP-133040X Nature structural & molecular biology 2009 IMEx Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nature structural & molecular biology 2009 imex curation Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. DIP original experiment: DIP-133038X Nature structural & molecular biology 2009 IMEx Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nature structural & molecular biology 2009 imex curation Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. DIP original experiment: DIP-133039X Nature structural & molecular biology 2009 IMEx Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nature structural & molecular biology 2009 imex curation Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Reuter M., Chuma S., Tanaka T., Franz T., Stark A., Pillai RS. DIP original experiment: DIP-133042X Nature structural & molecular biology 2009 IMEx Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. piwl2_mouse Piwi-like protein 2 Piwil2 Mili protein protein mouse Mus musculus Mouse MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEDSSPPLQPVQKDSVGLVSMFRGMGLDTAFRPPSKREVPPLGRGVLGRGLSANMVRKDREEPRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSEVSLLPLGRAASSIGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERKEKELLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNVTAFDGSILYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL DIP protein Q8CDG1 original sequence version: 50 mint 01E143C6513310FB tdrd1_mouse Tudor domain-containing protein 1 Tdrd1 protein protein mouse Mus musculus Mouse MMPRNNLEASTCKMAEPFNFEKKESKPPPQDPLRSPVAQHNHPTFRLKSPENGNTKNNFLLCEQNKQYLASQEDSSVVSSNPAVVNGEVGGSKGDRKPPPTGNPVSPLSLGNSSPPNQVKTKPSSNVTPEKSKKSHKLFENALSVNNPALFNSLGPPLRSTTCHRCGLFGSLRCSQCKQTYYCSTACQRRDWSSHSTICRPVQQSLNKLEDNKSPFETKAIEVKSEVDCPPGVTKEITAGAERVMFSDLRSLQLKKTMEIKGTVTEFKHPSNFYIQLYSSEVLENMNQLSTSLKETYANVVPEDGYLPVKGEVCVAKYTVDQTWNRAIVQAVDVLQRKAHVLYIDYGNEEMIPIDSVHPLSRGLDLFPPSAIKCCVSGVIPTAGEWSEGCVAAVKALLFEQFCSVKVMDILEEEVLTCAVDLVLQSSGKQLDHVLVEMGYGVKPGEQSSTEQSVDHSALEDVGRVTVESKIVTDRNALIPKVLTLNVGDEFCGVVAHIQTPEDFFCQQLQSGHKLAELQESLSEYCGHVIPRSDFYPTIGDVCCAQFSEDDQWYRASVLAYASEESVLVGYVDYGNFEILSLKRLCPIIPKLLDLPMQALNCVLAGVKPSLGIWTPEAVCVMKEMVQNRMVTVRVVGMLGTRALVELIDKSVAPHVSASKALIDSGFAIKEKDVADKGSSMHTASVPLAIEGPAEALEWTWVEFTVDETVDVVVCMMYSPGEFYCHFLKDDALEKLDDLNQSLADYCAQKPPNGFKAEIGRPCCAFFSGDGNWYRALVKEILPSGNVKVHFVDYGNVEEVTTDQLQAILPQFLLLPFQGMQCWLVDIQPPNKHWTKEATARFQACVVGLKLQARVVEITANGVGVELTDLSTPYPKIISDVLIREQLVLRCGSPQDSLMSRPANQHKQIDSHRVQASPSAEQWKTMELPVNKTIAANVLEIISPALFYAIPSEMSENQEKLCVLAAELLEHCNAQKGQPAYRPRTGDACCAKYTNDDFWYRAIVLETSESDVKVLYADYGNIETLPLSRVQPIPASHLELPFQIIRCSLEGPMELNGSCSQLVMELLRNAMLNQSVVLSVKAISKNVHAVSVEKCSENGMINIAENLVMCGLAENLTSKRKSASTKEIPHSRDCCCTELQKQIEKHEQILLFLLNNPTNQSKFTEMKKLLRS DIP protein Q99MV1 original sequence version: 58 mint EF42786CAD71933D piwil2-tdrd1-3 4 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 7 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association piwil2-tdrd1-4 2 8 unspecified role unspecified role prey prey Tudor 1-4 domain Region 307-1040 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait Region 69-80 Region 69-80 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro direct interaction direct interaction piwil2-tdrd1-5 1 8 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Tudor 1-4 domain Region 307-1040 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 7 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal domain Region 1-385 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association piwil2-tdrd1-6 5 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 7 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association piwil2-tdrd1-7 6 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 7 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association tdrd1-piwil2-4 3 8 unspecified role unspecified role bait bait mouse Mus musculus Mouse 7 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association