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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Direct observation of ATP-induced conformational changes in single P2X(4) receptors.</fullName>
        </names>
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            <attribute name="publication title" nameAc="MI:1091">Direct observation of ATP-induced conformational changes in single P2X(4) receptors.</attribute>
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            <attribute name="publication year" nameAc="MI:0886">2009</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Shinozaki Y.,  Sumitomo K.,  Tsuda M.,  Koizumi S.,  Inoue K.,  Torimitsu K.</attribute>
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          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>comig non denat gel</shortLabel>
            <fullName>comigration in non denaturing gel electrophoresis</fullName>
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          <xref>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>weight silver stain</shortLabel>
            <fullName>molecular weight estimation by silver staining</fullName>
            <alias type="synonym" typeAc="MI:1041">weight silver stain</alias>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0817" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Shinozaki Y., Sumitomo K., Tsuda M., Koizumi S., Inoue K., Torimitsu K.</attribute>
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          <attribute name="journal" nameAc="MI:0885">PLoS biology</attribute>
          <attribute name="publication year" nameAc="MI:0886">2009</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
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      </experimentDescription>
      <experimentDescription id="2">
        <names>
          <fullName>Direct observation of ATP-induced conformational changes in single P2X(4) receptors.</fullName>
        </names>
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            <names>
              <shortLabel>human</shortLabel>
              <fullName>Homo sapiens</fullName>
              <alias type="synonym" typeAc="MI:1041">Human</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>atomic force microsc</shortLabel>
            <fullName>atomic force microscopy</fullName>
            <alias type="go synonym" typeAc="MI:0303">AFM</alias>
            <alias type="synonym" typeAc="MI:1041">AFM</alias>
            <alias type="synonym" typeAc="MI:1041">atomic force microsc</alias>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
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            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Shinozaki Y., Sumitomo K., Tsuda M., Koizumi S., Inoue K., Torimitsu K.</attribute>
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          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
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        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="3">
        <names>
          <shortLabel>p2rx4_rat</shortLabel>
          <fullName>P2X purinoceptor 4</fullName>
          <alias type="gene name" typeAc="MI:0301">P2rx4</alias>
          <alias type="gene name synonym" typeAc="MI:0302">ATP receptor</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Purinergic receptor</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P51577" version="SP_109" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSRNOP00000001752" version="SP_158" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-9511617" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="rgd" dbAc="MI:0483" id="62073"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_113782.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-48411N"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSRNOG00000001300" version="SP_158" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSRNOT00000001752" version="SP_158" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0001614" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0002028" version="SP_158"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005102" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005507" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005524" version="SP_158"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008270" version="SP_158"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019228" version="SP_158"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0055117" version="SP_158"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0060079" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0070588" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0071294" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0071318" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0097190" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0098662" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0099604" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1904141" version="SP_158"/>
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          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027309" version="SP_158"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2BP5" version="SP_158"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2RUP" version="SP_158"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-RNO-139853" version="SP_158"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-RNO-418346" version="SP_158"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
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        </interactorType>
        <organism ncbiTaxId="10116">
          <names>
            <shortLabel>rat</shortLabel>
            <fullName>Rattus norvegicus (Rat)</fullName>
            <alias type="synonym" typeAc="MI:1041">Rat</alias>
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        </organism>
        <sequence>MAGCCSVLGSFLFEYDTPRIVLIRSRKVGLMNRAVQLLILAYVIGWVFVWEKGYQETDSVVSSVTTKAKGVAVTNTSQLGFRIWDVADYVIPAQEENSLFIMTNMIVTVNQTQSTCPEIPDKTSICNSDADCTPGSVDTHSSGVATGRCVPFNESVKTCEVAAWCPVENDVGVPTPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNITTSYLKSCIYNAQTDPFCPIFRLGTIVEDAGHSFQEMAVEGGIMGIQIKWDCNLDRAASLCLPRYSFRRLDTRDLEHNVSPGYNFRFAKYYRDLAGKEQRTLTKAYGIRFDIIVFGKAGKFDIIPTMINVGSGLALLGVATVLCDVIVLYCMKKKYYYRDKKYKYVEDYEQGLSGEMNQ</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P51577 original sequence version: 79</attribute>
          <attribute name="crc64">C7B0FC9A94EF8D9A</attribute>
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      </interactor>
    </interactorList>
    <interactionList>
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          <shortLabel>p2rx4-1</shortLabel>
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        <participantList>
          <participant id="5">
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            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
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            <experimentalRoleList>
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                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human</shortLabel>
                  <fullName>Homo sapiens</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
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        </interactionType>
      </interaction>
      <interaction id="6" imexId="IM-14157-2">
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        <participantList>
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            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
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                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human</shortLabel>
                  <fullName>Homo sapiens</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>