DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249876X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249878X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249880X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249879X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249877X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249875X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249881X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 2009 imex curation Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dong X., Biswas A., Süel KE., Jackson LK., Martinez R., Gu H., Chook YM. DIP original experiment: DIP-249874X Nature 2009 IMEx Structural basis for leucine-rich nuclear export signal recognition by CRM1. xpo1_human Exportin-1 CRM1 XPO1 Chromosome region maintenance 1 protein homolog protein protein human Homo sapiens Human MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKSKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFVTLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPTQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTSLNPGNPVNNQIFLQEYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEEREIALRQADEEKHKRQMSVPGIFNPHEIPEEMCD DIP protein O14980 original sequence version: 98 DIP protein O14980 original sequence version: 120 DIP protein O14980 original sequence version: 97 DIP protein O14980 original sequence version: 96 DIP protein O14980 original sequence version: 116 DIP protein O14980 original sequence version: 129 DIP protein O14980 original sequence version: 130 FDB00C065DA2FB1D rev_hv1h2 Protein Rev rev ART/TRS Anti-repression transactivator Regulator of expression of viral proteins protein protein hv1h2 Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) HIV-1 MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILGTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE DIP protein P04618 original sequence version: 105 DIP protein P04618 original sequence version: 107 4BCF5059C9A7F3B2 nmd3_human 60S ribosomal export protein NMD3 NMD3 CGI-07 protein protein human Homo sapiens Human MEYMAESTDRSPGHILCCECGVPISPNPANICVACLRSKVDISQGIPKQVSISFCKQCQRYFQPPGTWIQCALESRELLALCLKKIKAPLSKVRLVDAGFVWTEPHSKRLKVKLTIQKEVMNGAILQQVFVVDYVVQSQMCGDCHRVEAKDFWKAVIQVRQKTLHKKTFYYLEQLILKYGMHQNTLRIKEIHDGLDFYYSSKQHAQKMVEFLQCTVPCRYKASQRLISQDIHSNTYNYKSTFSVEIVPICKDNVVCLSPKLAQSLGNMNQICVCIRVTSAIHLIDPNTLQVADIDGSTFWSHPFNSLCHPKQLEEFIVMECSIVQDIKRAAGAGMISKKHTLGEVWVQKTSEMNTDKQYFCRTHLGHLLNPGDLVLGFDLANCNLNDEHVNKMNSDRVPDVVLIKKSYDRTKRQRRRNWKLKELARERENMDTDDERQYQDFLEDLEEDEAIRKNVNIYRDSAIPVESDTDDEGAPRISLAEMLEDLHISQDATGEEGASMLT DIP protein Q96D46 original sequence version: 73 5CEFD4E41FE2EBC1 spn1_human Snurportin-1 RNA U transporter 1 SNUPN RNUT1 SPN1 protein protein human Homo sapiens Human MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIMEHKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN DIP protein O95149 original sequence version: 107 DIP protein O95149 original sequence version: 100 DIP protein O95149 original sequence version: 80 DIP protein O95149 original sequence version: 84 50B456D1C23B4BA1 snupn-xpo1-1 2 12 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role prey prey O14980:p.[Ile521Ala;Leu525Ala;Phe561Ala;Phe572Ala] I521A/L525A/F561A/F572A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I521A/L525A/F561A/F572A'. Wed May 02 17:16:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile521ala,leu525ala,phe561ala,phe572ala' Tue Jun 30 16:12:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile521Ala;Leu525Ala;Phe561Ala;Phe572Ala]' ecolx Escherichia coli physical association physical association snupn-xpo1-2 8 12 unspecified role unspecified role unspecified role unspecified role Region 1-342 Region 1-342 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction revnes-xpo1-1 3 10 unspecified role unspecified role bait bait Region 72-85 Region 72-85 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey O14980:p.[Ile521Ala;Leu525Ala;Phe561Ala;Phe572Ala] I521A/L525A/F561A/F572A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I521A/L525A/F561A/F572A'. Wed May 02 19:32:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile521ala,leu525ala,phe561ala,phe572ala' Tue Jun 30 17:36:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile521Ala;Leu525Ala;Phe561Ala;Phe572Ala]' ecolx Escherichia coli physical association physical association snupn-xpo1-3 5 12 unspecified role unspecified role bait bait Region 1-12 Region 1-12 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O95149:p.[Leu4Ala;Leu8Ala] L4A/L8A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L4A/L8A'. Wed May 02 17:29:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu4ala,leu8ala' Tue Jun 30 19:59:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu4Ala;Leu8Ala]' O95149:p.[Glu2Lys;Glu3Lys;Ser11Ala] E2K/E3K/S11A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'E2K/E3K/S11A'. Wed May 02 17:22:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu2lys,glu3lys,ser11ala' Tue Jun 30 16:57:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu2Lys;Glu3Lys;Ser11Ala]' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O95149:p.[Lys144Asp;Gln181Ala;Lys221Ala] K144D/Q181A/K221A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain K D This feature has been corrected as a result of our quality control procedures. The original label was 'K144D/Q181A/K221A'. Wed May 02 15:35:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys144asp,gln181ala,lys221ala' Tue Jun 30 15:48:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys144Asp;Gln181Ala;Lys221Ala]' O95149:p.[Lys144Asp;Tyr147Ala;Asn150Ala;Glu178Ala;Gln181Ala] K144D/Y147A/N150A/E178A/Q181A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain K D This feature has been corrected as a result of our quality control procedures. The original label was 'K144D/Y147A/N150A/E178A/Q181A'. Wed May 02 14:51:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys144asp,tyr147ala,asn150ala,glu178ala,gln181ala' Tue Jun 30 16:37:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys144Asp;Tyr147Ala;Asn150Ala;Glu178Ala;Gln181Ala]' ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction snupn-xpo1-4 6 12 unspecified role unspecified role unspecified role unspecified role Region 1-342 Region 1-342 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nmd3-xpo1-1 4 11 unspecified role unspecified role bait bait Region 477-491 Region 477-491 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey O14980:p.[Ile521Ala;Leu525Ala;Phe561Ala;Phe572Ala] I521A/L525A/F561A/F572A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I521A/L525A/F561A/F572A'. Wed May 02 16:38:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile521ala,leu525ala,phe561ala,phe572ala' Tue Jun 30 19:51:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile521Ala;Leu525Ala;Phe561Ala;Phe572Ala]' ecolx Escherichia coli physical association physical association xpo1-snupn-1 7 12 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait O14980:p.[Gln581Ala;Asp624Lys;Asp681Ala;Glu683Ala;Gln687Ala] Q581/D624K/D681A/E683A/Q687A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D K certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain E A Fri Jul 28 10:55:07 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gn581ala,asp624lys,asp681ala,glu683ala,gln687ala' Wed May 02 20:29:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln581ala,asp624lys,asp681ala,glu683ala,gln687ala' Tue Jun 30 17:18:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln581Ala;Asp624Lys;Asp681Ala;Glu683Ala;Gln687Ala]' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction snupn-xpo1-5 1 12 unspecified role unspecified role unspecified role unspecified role Region 1-342 Region 1-342 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction