DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Pan L., Yan J., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Pan L., Yan J., Wu L., Zhang M. DIP original experiment: DIP-134037X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Pan L., Yan J., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Pan L., Yan J., Wu L., Zhang M. DIP original experiment: DIP-134040X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Pan L., Yan J., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Pan L., Yan J., Wu L., Zhang M. DIP original experiment: DIP-134038X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Pan L., Yan J., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Pan L., Yan J., Wu L., Zhang M. DIP original experiment: DIP-134039X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Pan L., Yan J., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Pan L., Yan J., Wu L., Zhang M. DIP original experiment: DIP-134036X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Pan L., Yan J., Wu L., Zhang M. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Pan L., Yan J., Wu L., Zhang M. DIP original experiment: DIP-134035X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Assembling stable hair cell tip link complex via multidentate interactions between harmonin and cadherin 23. ush1c_human Harmonin Usher syndrome type-1C protein Autoimmune enteropathy-related antigen AIE-75 Antigen NY-CO-38/NY-CO-37 Protein PDZ-73 Renal carcinoma antigen NY-REN-3 USH1C AIE75 protein protein human Homo sapiens Human MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEAVNVLKSSRSLTISIVAAAGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPELEPADDLDGGTEEQGEQDFRKYEEGFDPYSMFTPEQIMGKDVRLLRIKKEGSLDLALEGGVDSPIGKVVVSAVYERGAAERHGGIVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF DIP protein Q9Y6N9 original sequence version: 95 DIP protein Q9Y6N9 original sequence version: 91 7E75CEE873C57F41 cad23pbm Cadherin-23 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ETPLEITEL cad23ct Cadherin-23 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KPDDDRYLRAAIQEYDNIAK q9h251-4 Cadherin-23 CDH23 KIAA1774 KIAA1812 Otocadherin UNQ1894/PRO4340 protein protein human Homo sapiens Human MGRHVATSCHVAWLLVLISGCWGQVNRLPFFTNHFFDTYLLISEDTPVGSSVTQLLAQDMDNDPLVFGVSGEEASRFFAVEPDTGVVWLRQPLDRETKSEFTVEFSVSDHQGVITRKVNIQVGDVNDNAPTFHNQPYSVRIPENTPVGTPIFIVNATDPDLGAGGSVLYSFQPPSQFFAIDSARGIVTVIRELDYETTQAYQLTVNATDQDKTRPLSTLANLAIIITDVQDMDPIFINLPYSTNIYEHSPPGTTVRIITAIDQDKGRPRGIGYTIVSGNTNSIFALDYISGVLTLNGLLDRENPLYSHGFILTVKGTELNDDRTPSDATVTTTFNILVIDINDNAPEFNSSEYSVAITELAQVGFALPLFIQVVDKDENLGLNSMFEVYLVGNNSHHFIISPTSVQGKADIRIRVAIPLDYETVDRYDFDLFANESVPDHVGYAKVKITLINENDNRPIFSQPLYNISLYENVTVGTSVLTVLATDNDAGTFGEVSYFFSDDPDRFSLDKDTGLIMLIARLDYELIQRFTLTIIARDGGGEETTGRVRINVLDVNDNVPTFQKDAYVGALRENEPSVTQLVRLRATDEDSPPNNQITYSIVSASAFGSYFDISLYEGYGVISVSRPLDYEQISNGLIYLTVMAMDAGNPPLNSTVPVTIEVFDENDNPPTFSKPAYFVSVVENIMAGATVLFLNATDLDRSREYGQESIIYSLEGSTQFRINARSGEITTTSLLDRETKSEYILIVRAVDGGVGHNQKTGIATVNITLLDINDNHPTWKDAPYYINLVEMTPPDSDVTTVVAVDPDLGENGTLVYSIQPPNKFYSLNSTTGKIRTTHAMLDRENPDPHEAELMRKIVVSVTDCGRPPLKATSSATVFVNLLDLNDNDPTFQNLPFVAEVLEGIPAGVSIYQVVAIDLDEGLNGLVSYRMPVGMPRMDFLINSSSGVVVTTTELDRERIAEYQLRVVASDAGTPTKSSTSTLTIHVLDVNDETPTFFPAVYNVSVSEDVPREFRVVWLNCTDNDVGLNAELSYFITGGNVDGKFSVGYRDAVVRTVVGLDRETTAAYMLILEAIDNGPVGKRHTGTATVFVTVLDVNDNRPIFLQSSYEASVPEDIPEGHSILQLKATDADEGEFGRVWYRILHGNHGNNFRIHVSNGLLMRGPRPLDRERNSSHVLIVEAYNHDLGPMRSSVRVIVYVEDINDEAPVFTQQQYSRLGLRETAGIGTSVIVVQATDRDSGDGGLVNYRILSGAEGKFEIDESTGLIITVNYLDYETKTSYMMNVSATDQAPPFNQGFCSVYITLLNELDEAVQFSNASYEAAILENLALGTEIVRVQAYSIDNLNQITYRFNAYTSTQAKALFKIDAITGVITVQGLVDREKGDFYTLTVVADDGGPKVDSTVKVYITVLDENDNSPRFDFTSDSAVSIPEDCPVGQRVATVKAWDPDAGSNGQVVFSLASGNIAGAFEIVTTNDSIGEVFVARPLDREELDHYILQVVASDRGTPPRKKDHILQVTILDINDNPPVIESPFGYNVSVNENVGGGTAVVQVRATDRDIGINSVLSYYITEGNKDMAFRMDRISGEIATRPAPPDRERQSFYHLVATVEDEGTPTLSATTHVYVTIVDENDNAPMFQQPHYEVLLDEGPDTLNTSLITIQALDLDEGPNGTVTYAIVAGNIVNTFRIDRHMGVITAAKELDYEISHGRYTLIVTATDQCPILSHRLTSTTTVLVNVNDINDNVPTFPRDYEGPFEVTEGQPGPRVWTFLAHDRDSGPNGQVEYSIMDGDPLGEFVISPVEGVLRVRKDVELDRETIAFYNLTICARDRGMPPLSSTMLVGIRVLDINDNDPVLLNLPMNITISENSPVSSFVAHVLASDADSGCNARLTFNITAGNRERAFFINATTGIVTVNRPLDRERIPEYKLTISVKDNPENPRIARRDYDLLLIFLSDENDNHPLFTKSTYQAEVMENSPAGTPLTVLNGPILALDADQDIYAVVTYQLLGAQSGLFDINSSTGVVTVRSGVIIDREAFSPPILELLLLAEDIGLLNSTAHLLITILDDNDNRPTFSPATLTVHLLENCPPGFSVLQVTATDEDSGLNGELVYRIEAGAQDRFLIHLVTGVIRVGNATIDREEQESYRLTVVATDRGTVPLSGTAIVTILIDDINDSRPEFLNPIQTVSVLESAEPGTVIANITAIDHDLNPKLEYHIVGIVAKDDTDRLVPNQEDAFAVNINTGSVMVKSPMNRELVATYEVTLSVIDNASDLPERSVSVPNAKLTVNVLDVNDNTPQFKPFGITYYMERILEGATPGTTLIAVAAVDPDKGLNGLVTYTLLDLVPPGYVQLEDSSAGKVIANRTVDYEEVHWLNFTVRASDNGSPPRAAEIPVYLEIVDINDNNPIFDQPSYQEAVFEDVPVGTIILTVTATDADSGNFALIEYSLGDGESKFAINPTTGDIYVLSSLDREKKDHYILTALAKDNPGDVASNRRENSVQVVIQVLDVNDCRPQFSKPQFSTSVYENEPAGTSVITMMATDQDEGPNGELTYSLEGPGVEAFHVDMDSGLVTTQRPLQSYEKFSLTVVATDGGEPPLWGTTMLLVEVIDVNDNRPVFVRPPNGTILHIREEIPLRSNVYEVYATDKDEGLNGAVRYSFLKTAGNRDWEFFIIDPISGLIQTAQRLDRESQAVYSLILVASDLGQPVPYETMQPLQVALEDIDDNEPLFVRPPKGSPQYQLLTVPEHSPRGTLVGNVTGAVDADEGPNAIVYYFIAAGNEEKNFHLQPDGCLLVLRDLDREREAIFSFIVKASSNRSWTPPRGPSPTLDLVADLTLQEVRVVLEDINDQPPRFTKAEYTAGVATDAKVGSELIQVLALDADIGNNSLVFYSILAIHYFRALANDSEDVGQVFTMGSMDGILRTFDLFMAYSPGYFVVDIVARDLAGHNDTAIIGIYILRDDQRVKIVINEIPDRVRGFEEEFIHLLSNITGAIVNTDNVQFHVDKKGRVNFAQTELLIHVVNRDTNRILDVDRVIQMIDENKEQLRNLFRNYNVLDVQPAISVRLPDDMSALQMAIIVLAILLFLAAMLFVLMNWYYRTVHKRKLKAIVAGSAGNRGFIDIMDMPNTNKYSFDGANPVWLDPFCRNLELAAQAEHEDDLPENLSEIADLWNSPTRTHGTFGREPAAVKPDDDRYLRAAIQEYDNIAKLGQIIREGPIKLIQTELDEEPGDHSPGQGSLRFRHKPPVELKGPDGIHVVHGSTGTLLATDLNSLPEEDQKGLGRSLETLTAAEATAFERNARTESAKSTPLHKLRDVIMETPLEITEL DIP protein Q9H251-4 original sequence version: 96 ush1c-cdh23-1 6 10 unspecified role unspecified role prey prey Region 3104-3319 Region 3104-3319 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 1-97 Region 1-97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction cad23ct-ush1c-1 4 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1-97 Region 1-97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cad23pbm-ush1c-1 1 8 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 208-299 Region 208-299 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cad23ct-ush1c-2 2 9 unspecified role unspecified role unspecified role unspecified role cad23ct:p.Leu8Gln L8Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L Q This feature has been corrected as a result of our quality control procedures. The original label was 'L8Q'. Wed May 02 14:50:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu8gln' Tue Jun 30 19:59:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu8Gln' cad23ct:p.Ile12Gln I12Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I Q This feature has been corrected as a result of our quality control procedures. The original label was 'I12Q'. Wed May 02 16:19:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile12gln' Tue Jun 30 19:09:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile12Gln' cad23ct:p.Tyr15Gln Y15Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y Q This feature has been corrected as a result of our quality control procedures. The original label was 'Y15Q'. Wed May 02 17:33:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr15gln' Tue Jun 30 19:55:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr15Gln' ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 1-97 Region 1-97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cad23ct-ush1c-3 3 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1-97 Region 1-97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cad23pbm-ush1c-2 5 8 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 208-299 Region 208-299 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction