DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture. Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mayanagi K., Kiyonari S., Saito M., Shirai T., Ishino Y., Morikawa K. Only protein-protein interactions in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Mayanagi K., Kiyonari S., Saito M., Shirai T., Ishino Y., Morikawa K. DIP original experiment: DIP-134004X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture. Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture. Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mayanagi K., Kiyonari S., Saito M., Shirai T., Ishino Y., Morikawa K. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Mayanagi K., Kiyonari S., Saito M., Shirai T., Ishino Y., Morikawa K. DIP original experiment: DIP-134003X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture. dnli_pyrfu DNA ligase lig Polydeoxyribonucleotide synthase [ATP] PF1635 protein protein pyrfu Pyrococcus furiosus MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGVGEKLLIKAVAMATGIDAKEIEESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTLVKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILGTMRTGVAEGLLRDAIAMAFHVKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEMGGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGELVAIGENGRPLPFQYVLRRFRRKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRTLEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGLMAKRLDAVYEPGNRGKKWLKIKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFTKMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDADTIERIAQLYELQEKMKGKVES 534158525B9D24B2 pcna_pyrfu DNA polymerase sliding clamp pcn Proliferating cell nuclear antigen homolog PF0983 protein protein pyrfu Pyrococcus furiosus MPFEIVFEGAKEFAQLIDTASKLIDEAAFKVTEDGISMRAMDPSRVVLIDLNLPSSIFSKYEVVEPETIGVNMDHLKKILKRGKAKDTLILKKGEENFLEITIQGTATRTFRVPLIDVEEMEVDLPELPFTAKVVVLGEVLKDAVKDASLVSDSIKFIARENEFIMKAEGETQEVEIKLTLEDEGLLDIEVQEETKSAYGVSYLSDMVKGLGKADEVTIKFGNEMPMQMEYYIRDEGRLTFLLAPRVEE B6932127B001FA74 pfulig-pfupcna-1 2 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association pfulig-pfupcna-2 1 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association