DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133779X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133781X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133778X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Crystal structure of the eIF4A-PDCD4 complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Chang JH., Cho YH., Sohn SY., Choi JM., Kim A., Kim YC., Jang SK., Cho Y. DIP original experiment: DIP-133780X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the eIF4A-PDCD4 complex. if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI DIP protein P60842 original sequence version: 125 DIP protein P60842 original sequence version: 59 DIP protein P60842 original sequence version: 63 DIP protein P60842 original sequence version: 85 DIP protein P60842 original sequence version: 86 DIP protein P60842 original sequence version: 92 DIP protein P60842-1 original sequence version: 114 DIP protein P60842 original sequence version: 106 6EF89939F3045420 pdcd4_human Programmed cell death protein 4 PDCD4 H731 Nuclear antigen H731-like Neoplastic transformation inhibitor protein Protein 197/15a protein protein human Homo sapiens Human MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY DIP protein Q53EL6 original sequence version: 26 DIP protein Q53EL6 original sequence version: 55 2CAE1D2055491177 eif4a1-pdcd4-6 3 6 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q53EL6:p.[Glu249Ala;Asp253Ala;Asp414Ala;Asp418Ala] E24A/E253A/D414A/D418A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain E A Wed May 02 20:19:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu249ala,asp253ala,asp414ala,asp418ala' Tue Jun 30 18:05:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu249Ala;Asp253Ala;Asp414Ala;Asp418Ala]' ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction eif4a1-pdcd4-7 4 6 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction eif4a1-pdcd4-8 1 6 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction eif4a1-pdcd4-9 2 6 unspecified role unspecified role unspecified role unspecified role Region 164-469 Region 164-469 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 20-406 Region 20-406 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction