DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132465X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132464X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132459X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132463X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132462X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132460X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Histone chaperone specificity in Rtt109 activation. Nature structural & molecular biology 2008 imex curation Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Park YJ., Sudhoff KB., Andrews AJ., Stargell LA., Luger K. DIP original experiment: DIP-132461X Nature structural & molecular biology 2008 IMEx Histone chaperone specificity in Rtt109 activation. vps75_yeast Vacuolar protein sorting-associated protein 75 N0890 YNL246W VPS75 protein protein yeast Saccharomyces cerevisiae Baker's yeast MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKIVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIFGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSEDDDGSLGEVDLPLSDEEPSSKKRKV 779F12CEA27B3E6B 779F12CEA27B3E6B rt109_yeast Histone acetyltransferase RTT109 RTT109 KIM2 REM50 Regulator of Ty1 transposition protein 109 L1377 YLL002W KAT11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGKVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYLQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFTCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQAKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEEDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRAIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLAITMLKPRKKAKALPKT 17825D6EF97C4BB5 17825D6EF97C4BB5 nap1_yeast Nucleosome assembly protein NAP1 YKR048C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDPIRTKPKSSMQIDNAPTPHNTPASVLNPSYLKNGNPVRAQAQEQDDKIGTINEEDILANQPLLLQSIQDRLGSLVGQDSGYVGGLPKNVKEKLLSLKTLQSELFEVEKEFQVEMFELENKFLQKYKPIWEQRSRIISGQEQPKPEQIAKGQEIVESLNETELLVDEEEKAQNDSEEEQVKGIPSFWLTALENLPIVCDTITDRDAEVLEYLQDIGLEYLTDGRPGFKLLFRFDSSANPFFTNDILCKTYFYQKELGYSGDFIYDHAEGCEISWKDNAHNVTVDLEMRKQRNKTTKQVRTIEKITPIESFFNFFDPPKIQNEDQDEELEEDLEERLALDYSIGEQLKDKLIPRAVDWFTGAALEFEFEEDEEEADEDEDEEEDDDHGLEDDDGESAEEQDDFAGRPEQAPECKQS C0F97FBA1B9EEA83 C0F97FBA1B9EEA83 rtt109-vsp75-1 5 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 1-223 Region 1-223 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction vsp75-1 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction vsp75-2 6 8 unspecified role unspecified role unspecified role unspecified role Region 1-223 Region 1-223 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rtt109-vsp75-2 1 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Alexa546-label Alexa546-label alexa 546 label alexa 546 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction nap1-rtt109-1 4 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rtt109-vsp75-3 7 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 1-223 Region 1-223 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rtt109-nap1-1 2 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association