DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133711X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133710X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133714X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133712X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133715X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133713X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Roux KJ., Crisp ML., Liu Q., Kim D., Kozlov S., Stewart CL., Burke B. DIP original experiment: DIP-133716X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization. kinh_human Kinesin-1 heavy chain KIF5B KNS KNS1 Ubiquitous kinesin heavy chain Conventional kinesin heavy chain protein protein human Homo sapiens Human MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV DIP protein P33176 original sequence version: 148 DIP protein P33176 original sequence version: 65 DIP protein P33176 original sequence version: 133 DIP protein P33176 original sequence version: 102 DIP protein P33176 original sequence version: 152 A1FE5760C3250C8B klc2_mouse Kinesin light chain 2 Klc2 protein protein mouse Mus musculus Mouse MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEMLPQEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDRRPMEYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGRGHRRGSRDVAGPQSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPNSRMKRASSLNFLNKSVEEPVQPGGRVFLTAAL DIP protein O88448 original sequence version: 114 DIP protein O88448 original sequence version: 80 A5E6F9081609E1CE klc4_mouse Kinesin light chain 4 Klc4 Knsl8 Kinesin-like protein 8 protein protein mouse Mus musculus Mouse MSGLVLGQRDEPAGHRLSQEEILGSTKVVSQGLEALHSEHQAVLQSLSHTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLRQLRQYDEDGHGMEEKEGEATKDSLDDLFPNEEEEDSGNDLSRGQGAAAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTDHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYESQLGPDNPNVARTKNNLASCYLKQGKYSEAEALYKEILTCAHVQEFGSVDDDHKPIWMHAEEREEMSRSRPRDSSAPYAEYGGWYKACRVSSPTVNTTLKNLGALYRRQGKLEAAETLEECALRSRKQGTDPISQTKVAELLGEGDGRKAIQEGPGDSVKFEGGEDASVAVEWSGDGSGTLQRSGSLGKIRDVLRRSSELLVRKLQGTEPRPSSSSMKRAASLNYLNQPNAAPLQVSRGLSASTVDLSSSS DIP protein Q9DBS5 original sequence version: 73 Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity mint BE2D6B3CD451A7BF klc1_mouse Kinesin light chain 1 Klc1 Kns2 protein protein mouse Mus musculus Mouse MYDNMSTMVYIKEEKLENVTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSSMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQNVRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDSDSSKEPLDDLFPNDEDEPGQGIQHSDSSAAAARQGYEIPARLRTLHNLVIQYASQGRYEVAVPSCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALGIYQTKLGPDRTPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEAEFGSVDDENKPIWMHAEEREECKGKQKDGSAFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPESMEKRRSRESLNMDVVKYESGPDGGEEA DIP protein O88447 original sequence version: 83 550F23AB36049C03 syne4_mouse Nesprin-4 Syne4 KASH domain-containing protein 4 Nuclear envelope spectrin repeat protein 4 protein protein mouse Mus musculus Mouse MALVPPLGREFPPEPVNCPLAAPRELDVVGGTICPAPEEETSRPEQVQASLGLPEHCMGELKSTESATSPSRLPLASSHEHQDGGKPCEHSDSGLEVLEAEQDSLHLCLLRLNFRLQDLERGLGSWTLAHNRIVQMQALQAELRGAAERVDALLAFGEGLAERSEPRAWASLEQVLRALGTHRDTIFQRLWQLQAQLISYSLVLEKANLLDQDLEVEGDSDGPAAGGVWGPWAPSTFPTPAELEWDPAGDVGGLGPSGQKISRIPGAPCELCGYRGPQSSGQGLEDLLSLGLGHRKHLAAHHRRRLRKPQDRKRQVSPSLPDAMLEVDRGVPAPASKRPLTLFFLLLFLLLVGATLLLPLSGVSCCSHARLARTPYLVLSYVNGLPPI DIP protein Q8CII8 original sequence version: 44 klc3_mouse Kinesin light chain 3 Klc3 protein protein mouse Mus musculus Mouse MSVQVAAPGSTGLGPERLNPEELVRQTRQVVQGLEALRAEHHSLAGHLAEALAGPGPVAGVELLEEKQQVVNHSLEAIELGLGEAQVLLALSAHVSVLEAEKQRLRAQARRLAQENTWLREELEETQRRLRASEEAVAQLEEEKSHLQFLGQLRQYDPPEESQRPESPPRRDSLASLFPSEEEEKKGPEAAGAAAAQQGGYEIPARLRTLHNLVIQYAGQGRYEVAVPLCRQALEDLERSSGHCHPDVATMLNILALVYRDQNKYKEATELLHDALQIREQTLGPEHPAVAATLNNLAVLYGKRGRYREAEPLCQRALEIREKVLGADHPDVAKQLNNLALLCQNQGKFQDVERHYARALSIYEALGGPQDPNVAKTKNNLASAYLKQNKYQQAEELYKEILSQEALPAPLGAPQGGTAGDTQQQVLRRSSSFSKLRESIRRGSEKLVSRLRGEGMAGAAGMKRAMSLNMLNVDGPRAARTQLSQLSTRHLSEAPRTLSISTQDLSPR DIP protein Q91W40 original sequence version: 63 Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport mint 7F6F11CB943792C2 klc1_rat Kinesin light chain 1 Klc1 Klc Kns2 protein protein rat Rattus norvegicus (Rat) Rat MHDNMSTMVYMKEEKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSSMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDSDSSKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGSSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAALRSRKQGLDNVHKQRVAEVLNDPENVEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNGMRKMKLGLVK DIP protein P37285 original sequence version: 83 24D0C4CF9E304366 syne4-klc1-1 4 14 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 12 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 1-338 Region 1-338 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association syne4-kif5b-1 2 8 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait Region 1-338 Region 1-338 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association syne4-klc2-1 3 9 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait Region 129-339 Region 129-339 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction syne4-kif5b-2 1 8 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait Region 207-307 Region 207-307 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association syne4-klc3-1 5 13 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait Region 129-339 Region 129-339 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction syne4-klc1-2 6 11 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait Region 129-339 Region 129-339 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction syne4-klc4-1 7 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait Region 129-339 Region 129-339 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction