DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216037X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216035X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216040X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216039X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216038X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216042X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216041X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Su JR., Takeda K., Tamura S., Fujiki Y., Miki K. DIP original experiment: DIP-216036X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p. 5p7 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EAHPWLSDYDDLT pex5r_human PEX5-related protein PEX5L PEX5R PXR2 PEX2-related protein PEX5-like protein Peroxin-5-related protein Peroxisome biogenesis factor 5-like Tetratricopeptide repeat-containing Rab8b-interacting protein protein protein human Homo sapiens Human MYQGHMQKSKEQGYGKLSSDEDLEIIVDQKQGKGSRAADKAVAMVMKEIPREESAEEKPLLTMTSQLVNEQQESRPLLSPSIDDFLCETKSEAIARPVTSNTAVLTTGLDLLDLSEPVSQTQTKAKKSEPSSKTSSLKKKADGSDLISTDAEQRGQPLRVPETSSLDLDIQTQLEKWDDVKFHGDRNTKGHPMAERKSSSSRTGSKELLWSSEHRSQPELSGGKSALNSESASELELVAPTQARLTKEHRWGSALLSRNHSLEEEFERAKAAVESDTEFWDKMQAEWEEMARRNWISENQEAQNQVTISASEKGYYFHTENPFKDWPGAFEEGLKRLKEGDLPVTILFMEAAILQDPGDAEAWQFLGITQAENENEQAAIVALQRCLELQPNNLKALMALAVSYTNTGHQQDACDALKNWIKQNPKYKYLVKSKKGSPGLTRRMSKSPVDSSVLEGVKELYLEAAHQNGDMIDPDLQTGLGVLFHLSGEFNRAIDAFNAALTVRPEDYSLWNRLGATLANGDRSEEAVEAYTRALEIQPGFIRSRYNLGISCINLGAYREAVSNFLTALSLQRKSRNQQQVPHPAISGNIWAALRIALSLMDQPELFQAANLGDLDVLLRAFNLDP DIP protein Q8IYB4 original sequence version: 71 5p3 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SPARWAEEYLEQS 5p4 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TTDRWYDDYHPEE 5p6 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TSEAWVDQFTRSG 5p2 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) NETDWSQEFIAEV pex14_rat Peroxisomal membrane protein PEX14 Pex14 PTS1 receptor-docking protein Peroxin-14 Peroxisomal membrane anchor protein PEX14 protein protein rat Rattus norvegicus (Rat) Rat MASSEQAEQPNQPSSSPGSENVVPREPLIATAVKFLQNSRVRQSPLATRRAFLKKKGLTDEEIDLAFQQSGTASDEPSPVGPATPVVPVQPPHLISQPYSPGGSRWRDYGALAIILAGIAFGFHQLYKKYLLPLILGGREDRKQLERMAASLSELSGSVAQTVTQVQTTLASVQELLRQQQQKVQELAHELAAAKATTSTNWILESQNINELKSEINSLKGLLLNRRQFPPSPSAPKIPSWQIPVKSPSPSSPAAVNHHSSSDISPVSNESPSSSPGKDGHSPEGSTATYRLLGPQEEGEGVVDVKGQVRMEVQGEEEKREDEEGEEDEDDDVSHVDEEDVLGVQREDRRGGDGQINEQVEKLRRPEGASNESERD This sequence has been withdrawn from Uniprot. C9DFFC0DD4DD45C8 5p5 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) QAEQWAAEFIQQQ 5p1 Pex5p peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LSENWAQEFLAAG 5p4-pex14p-1 4 12 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pex14p-pex5l-1 2 10 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 15 unspecified role unspecified role bait bait Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q642G4:p.Phe35Ala F35A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F35A'. Wed May 02 20:27:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe35ala' Tue Jun 30 19:22:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe35Ala' Q642G4:p.Phe52Ala F52A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F52A'. Wed May 02 18:04:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe52ala' Tue Jun 30 15:41:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe52Ala' Q642G4:p.[Phe35Ala;Phe52Ala] F35A/F52A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F35A/F52A'. Wed May 02 14:59:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe35ala,phe52ala' Tue Jun 30 17:32:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe35Ala;Phe52Ala]' ecolx Escherichia coli direct interaction direct interaction 5p6-pex14p-1 7 13 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction 5p7-pex14p-1 6 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction 5p2-pex14p-1 1 14 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction 5p3-pex14p-1 5 11 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction 5p5-pex14p-1 3 16 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction 5p1-pex14p-1 8 17 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 15 unspecified role unspecified role unspecified role unspecified role Region 25-70 Region 25-70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction