DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structure (London, England : 1993) 2008 imex curation Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc experimental particp experimental participant identification experimental particp Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Structure (London, England : 1993) 2008 Only protein-protein interactions imex curation Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structure (London, England : 1993) 2008 imex curation Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. DIP original experiment: DIP-132184X david.chuang@utsouthwetstern.edu Structure (London, England : 1993) 2008 IMEx Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structure (London, England : 1993) 2008 imex curation Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase predetermined predetermined participant predetermined Kato M., Wynn RM., Chuang JL., Tso SC., Machius M., Li J., Chuang DT. david.chuang@utsouthwetstern.edu Structure (London, England : 1993) 2008 Only protein-protein interactions imex curation pdk4_human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial PDK4 PDHK4 Pyruvate dehydrogenase kinase isoform 4 protein protein human Homo sapiens Human MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM C17B78B6057000E9 p08559-1 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHE1-A type I PDHA1 PHE1A protein protein human Homo sapiens Human MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS 4D685BBE44A92D4B p11177-1 Isoform 1 of Pyruvate dehydrogenase E1 component subunit beta, mitochondrial PDHB PHE1B protein protein human Homo sapiens Human MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI AB459B1259FBDBD3 pdha1-pdhb-3 2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component 31-359 binding region 31-359 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed Sep 16 10:50:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.S232A,S300A' P08559-1:p.Tyr118Phe Y89F mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y F Wed Sep 16 10:27:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Y118F' 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 1,Fig 3,Table 2 pdha1-pdhb-4 2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component 31-359 binding region 31-359 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Sep 18 22:40:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.S232A,S300A' 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pSer293 pho S1 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3,Table 2 pdha1-pdk4-1 3 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp enzyme enzyme unspecified role unspecified role purified purified 10-411 binding region 10-411 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range SUMO Tag SUMO Tag sumo tag sumo tag SUMO tag sumo tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 32p radiolabel 32p radiolabel 32p radiolabel 32p radiolabel P32 32P 32P P32 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P08559-1:p.[Ser232Ala;Ser300Ala] S323A,S300A mutation mutation certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed Sep 16 10:03:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser232Ala,Ser300Ala' P08559-1:p.[Ser232Ala;Ser293Ala] S232A,S293A mutation mutation certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed Sep 16 10:16:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser232Ala,Ser293Ala' ecoli Escherichia coli (strain K12) phosphotransfer phosphotransfer reaction phosphotransfer Fig 2 pdha1-pdhb-5 1 6 unspecified role unspecified role neutral component neutral component 128-265 binding region 128-265 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range xpress tag xpress tag xpress tag xpress tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed Sep 16 10:40:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.S232A,S300A' 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 6 pdha1-pdhb-6 1 6 unspecified role unspecified role neutral component neutral component 128-265 binding region 128-265 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range xpress tag xpress tag xpress tag xpress tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component P08559-1:p.[Ser232Ala;Ser300Ala] S271A,S203A mutation mutation substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Sep 18 23:33:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.S232A,S300A' 30-390 binding region 30-390 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range opser opser opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 6