DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260489X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260492X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260491X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260495X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260494X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260493X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Powell E., Xu W. DIP original experiment: DIP-260490X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Powell E., Xu W. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Powell E., Xu W. DIP original experiment: DIP-260496X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Intermolecular interactions identify ligand-selective activity of estrogen receptor alpha/beta dimers. esr2_human Estrogen receptor beta ESR2 ESTRB NR3A2 Nuclear receptor subfamily 3 group A member 2 protein protein human Homo sapiens Human MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDADSSRKLAHLLNAVTDALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKCKNVVPVYDLLLEMLNAHVLRGCKSSITGSECSPAEDSKSKEGSQNPQSQ DIP protein Q92731 original sequence version: 171 8CAE34215992418A p03372-1 Estrogen receptor ESR1 ESR NR3A1 ER-alpha Estradiol receptor Nuclear receptor subfamily 3 group A member 1 ER66 Long hER-alpha66 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372-1 original sequence version: 214 DIP protein P03372-1 original sequence version: 139 DIP protein P03372-1 original sequence version: 204 DIP protein P03372-1 original sequence version: 171 DIP protein P03372-1 original sequence version: 179 DIP protein P03372-1 original sequence version: 206 esr1-7 3 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 10 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction esr1-esr2-1 1 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role fluorescence donor fluorescence donor P03372-1:p.Gly521Arg G521R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G R This feature has been corrected as a result of our quality control procedures. The original label was 'G521R'. Wed May 02 16:48:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly521arg' Tue Jun 30 16:38:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly521Arg' Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction esr2-esr1-1 6 9 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction esr1-esr2-2 4 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction esr2-1 2 9 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction esr2-esr1-2 5 9 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction esr1-esr2-3 7 9 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association esr1-8 8 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 10 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction