DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133372X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133377X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133375X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133373X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133371X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133374X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133370X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions 9tryp Trypanosoma brucei anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133376X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. q57ut3_tryb2 Serine/threonine-protein kinase TOR Tb927.4.420 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSLGYDARKVEALIRTIAVSSKCDVIYAAEVVKQAKDNQRKNQQEYENLVSCLLFCLENFDVVEGGTAEVKCALVTLATLLQLDLTANQVSQCNSYLSICLTHVQDVSVAFVASRAFGETLVTSMTSDFVQEQVEGAFLAIDLKENITHAQRVCALLMLQEVVTKIPMRILDMLDSLFEKLWGMLASSDAMIREISHLLFMDCGKLLCSRSPVNRKKTVDSLLHQLKFNLAAKSRESNMAGLLAFETVVMTSVGTASQPRYEDMSIMLVPYVMTGGSTNIAELRELLFRCLIVLCRYSTTLFITNQLKDTVGFALKSIENGYQVAASFNMLSEIIVLVTDVAFRPFVNETCNAVRYVSGKSSKPCWEAVRCFSVICHVCPPVDVESYIEPCIENIFRWGLSLPLIESMPHIIASSSAKYRSRLEEGLLDMISVTLCGLPFRQQPGSKGTITMKVESEPTESQITVALDALVQFGFSDSELMGDFLRDSVLPLIDSESVSVRTAAVRTICTLLIPPGVKGELTMARRICVDVILSRMLVVGLSNPDPVVRKGILSSFTEPFYPFLKEVQFVTSIYAALGDEDISCRVAATVLLCRMTFHDPSHILPIFRKQIVQILHTVSTSDNTGAVQSGLQLLDAAASNAPQFVMNFADGIIKALGHHFSTVNIHSSILQHLLRCCTSVAHAAHFFGNTDAIFRVEVDRAYMLLKALPLDSESVELRLWCLRFLSATLGPQIEGRSPYEVYPHLYQQLSGIVKNNDEDLDVRLEALRCAGVIGALDISIFHSTVVNRTCRHEEMKRNEPSTLSHAMCCRVVIRSIASLLDLSEKRFSGGKDNVLRVGIQTILNIAESCPDSRGEIAVVIPPVVRAVSELSACRLLGTLLHELTQIIRYVGVAALSHAHLLYNLIDESWVKSSKYRFLAVRLALTLVTLEADGKAESRRSNMRMSPIKFDALNDIESSESLRYAIIEYILKHTQTLKTCVETVVTNLLRAAQRCPIDFALSVITTLRVVCFRLHVDEFAGLIVRGLLSCLKTRLSTSMRATEQNAFVSRIMGVFCVLMTVLQSEFVKYSSEVLRTLKTLRVSNNELITLHGTLMKGGVCTLSKGFVTEHQKQVVRLLKECEGIALRHVSDLDKVLSTRGNVDEKGWNSDDGERPLPFSEKLIISTTRTAPLTKEEWLRWLNDFSCVIMQESPYRVFRCISLPLTANTSPLVDCSPQFTQDIAPHAFRALWNFASASVRSSLADFFFQVLQQAMESSAVPDEVVSALLTVAEYMDNVGMALPIAPSILSRCAWGRGMLAKALYWCEAAYRKDPRGTAKYLFSLYRELQQSGLLMVTGHSESLKLLQGCIDNERSESDGSDEYSVKWLSRQESLRRMCGVGGRSRSDANSSFNGEDVKLDPEMENEVNTLYALSEAGDYDAVLEQWKVLFQKYNGRDEQKREENTLFYVSQCAADASIRLQAWDTLEDTTRWQLNDTVSYHVSRAALSIHKRRYEEATSAINEGRKLLLEDLSGLLHQSYTRAYEGLVIAQKLSELEEIVTVKRAEVVSSTHHLSRVLRLWSQRIEMMSSTVSAWKEVLGVRGLLIPPGEDVATRLRFVNLCRREKAKQHERFILCQLLGYRCPTSEQLMHSNANPRVVMQYISFLSANGELGPKSSYGLERDLLKRMIDTHSRNENAFLLSSAYARLGSSAELSEAVQCYKAATTYCPKWFHAWRMFAESNAEMLDVTFSDATCAAAIEGYIQSIKLGNSDSTIIQDVLKLLTLLSRHCDQESGLKELRKRVFEVSPRAWKLVVPQLIARLDSGSDGSCELVAEILTTVSFDCPLSLIYPLNLCAMSNSRRRRKYANIILDKLQSKHPLVVNQGRILVDELVRTSDLLHEQWYHMLECSATAFFDRNDYKGMLDVLLPLHEKLHLSTDTIVEAEFTCKFGNKLDRAQEWLQSYLRTGSIADLHSAWNIYHAVYQQIDKQIRSQSKLHLSFCSPKLFEARNLAVGLPDAMPSEDCAVRCTASFNGTLTVIGSKQRPKRLSVTTVDGETQKYLLKGREDLRLDERVMQLFRLVNTLMMTDSRTCKNPGFQIQRYSVTPLKDTVGLIGWVSGCDTLHTLVTKYRKFRGIPPELELRMMNQIIVCDRPKTYDHLTMISKVEVMEFLADHTSGHDVRKAMWASASNCEMWLEQRQVFTTSSATTCMVGYILGLGDRHPNNVMIQRTSGLFVHIDFGDCFEVAMTRQMFPERVPFRLTRMIRNALDVFGVDGPFRSIAETAMCVLRDGSRSVLAILEAFIQDPLISWRLLNHMKKEPGDSTVHKNIDEQSEAVGPRMSSVAFPGAPVRDDMFTVDSKLTRCASIPDDDVVHKGVKVFQRVRSKLKGEEFLRTKGDTKSTDPKVQVARLIVEATDITNVAQSWAGWYPFW A3BCB24DC0FC8495 q57wf7_tryb2 Peptidylprolyl isomerase Tb927.7.3420 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSRNDCVMMDKIIEGDGKTIPRQGSIVTLDYVGYLPDGRKFDSTIERGKPFVFRVGCGEVIKGWDEGIVQMSKGERSRLTMPPSLAFGSTGFPGIIPPNTVIVFEVTLLDVV D28EDAA77792E620 q57yz3_tryb2 Tb927.8.3200 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVTTGLSPGALSLLQNAGINLKDFSRAQASYEKLCVELSKGASAAGEGDATAASSMEDLQRLCDAWGEYTVVAQAYFRKVSLAALEQLVSRPRGESSATERAEKLNELCAIGEKWHVGRETMFKLDHPEKLAAVVVEALDSSSCIMRLLALRLARIFAADTEFLRCLFSKGGAGLIGMCFDHENTTELEYVLSLCRRLIELHKASAEPYHELPFGWVYRIAALLDLQDPVPNLLLKRRSQALCVAVMLLRQFPQMATTASLHITLLRYCIEKGAATRDEVQSVLEVILDMFDSPETRQYLGYNDLDVLYAPFLYSTNMCSTEDLTLMNGAKEVLVMLMRNWVGVLWVCSETKGLRAVIDILHLPCDLDRKMVLLTLFNKLLYQLAPHRGITLVEPWRGFKEKQELFPATPNEPADSEGSFVLNTPLGRGHNSSESMISEHVFGDWCDEPDEFVPTTKAIGYHVLDPILGRVLLMLSHHGLPIALVSVIQDPNSSSVLTHAALSLLQDMLTLMDTVLPEKPVKRLHTALNKAVGRLALEGCLSLEGVLSSRIPQRGKTAKGIATTATVAVGNVVGSGWVSALSTASVATYGLGALEQDDADFKRMLKDTNVEGGNSFLSWNYDLLLLLFQGPLKSIRRFRWVLNETLFFRNLIAFYLPVINTSTRTFLTLQPREATPQVCMLGMVMLDLFLSTREGTAALDSFGFSGAMAACLEEVIRGTPYVLNKQRVGKRVGQTLLRMVGRYSMTANGLVVLKDSNMFSTINRMFVELSGERVSAPYPDDTLQEVCLQLLRCMNLGAVPNYGVCEWIRQMFRSALCNESDEIRLCAVKQLRDALWRDFSTSMRWGIETLVQALHDDHSRVVECAFKLLLNVCFCSDEALDYLISLSPTVLMESDTIRENAKQLDLNTLLYCIVGRPSGFRFLQCYGWVEQELRRWEETESANHVTTVENMQSGRALGSGADFREGMSALFRHRHNRTYSDTGLTFQKPITATAGPDSQVTLPSGIFPSHFAAVLCKSNEGCTLFKHSNLWQRSVRRILEQPMLPDIVYECGTFGERDNIDDDDVDMDGTTADDDDANALIEEDPSFVGRWGTEAAKTSTAKQDEKHALRLSREGHLPSSHSVKLLSASRGYQAAGQTYTIECVGDVSELKDAILCVCHAASSDAGFSLMRSVPMLQNQLMALSRFADTASVSSICIVGKCLLARSRRAAEKLTEISHYVVNESNAYMSADGVPYSVAFADNRPSNWTAIGRRDNPSRVSCSPPYFAHMRYDGEMVDGNSKNDEETTDSVGSTPSPVDSRDSSTFCDVIKGLSATSTRILHHIFALSNPVRRDAARQMLSQTLKHQPHIFVNPQMRWLLRHCAQAFRMRYDDRKFIASILEEAPLTKPTPLVRKTVVFVPEPQRG 9671512C9DDE1185 o76222_9tryp Non-specific serine/threonine protein kinase PI3K protein protein 9tryp Trypanosoma brucei SVAAVQVIVGIFSLEVLSPGCLVSFYGEVISSIQKQARVQVRKREEILVHLISLVRILKGHLRPHLKEITSTVDSFISVTDLSVLRQVLALLKELCCSLRRRFRPYMSSLLGPIIVLVEENVEETSEIVLDFFSAMGSLLEDHLHTVLPVVCNIIVDTSVPSRCRIVAVKTLICFTKRLPDLCFHASRCVHCLCRVLRESDGGDGVGDEGRLGCSAMEALCTLAGSLGKNFENFVLVVLPAVADRYGETSGEYCRFCHDIYEAIDGKRAPEVSSNGVGKAGGGGAPSLPFTAGTSASPLKDRADAYASLRFHLRKRDQASDEDWNHWLPQLAVNLLRSSSSPSHGRALTLAELHEPFARQMLHSAFAACYADMDEHTQREVIGLLTEVLRGLRVPSEVMQELLNLSEHMERQGIRLSAGGKASIKHSSNFCPFDRQVLMESSANCNLYSKALHYAEIEFLETVREYERSILRGCPKPLPVEDWQNLIKLCEKSIYFCNLLGQRESANGILKFIRQNLPLLTGKKVTELSQMMDAHLFDKLRWWSQSLQAYERRLQQEPNKVSNMVGLLRALDALGEYPRVLEMWRQFSKRVSKREVSKLASMGAHAAWLLRRWDDMEHITSFMSDEDYTGTTALFYKATLAARKKRFREAEKLIDMCRKRVDSKLSALVAESYDRAYDLFVGIQQLSELEELAMATSDPQSAMHWRQLWERRLSVMAYEGWPGTLANHTLLVPPSSEIDMWLRFVSLSRAHGQGSVSTEVLRELLGNQSIESAIENGIPTPAVAMGSFQHLYETNQRDSAIARLQLYVSKVEGSGAQHVSREREDMAVCHAKLAEWLVHQKKAHRTEDELQKIFHHLRRATELDKSNGSIWRTLARVHREAATKPADGSDSSGASGHIMEALSAYLRSVSLSEELEDALGFLSLWFMYGPLLAVQVGSTLKEEIEEVNPTVWLKVIPQIIARISSSNGTVADSVYNLLVLVARRHPQAILYSLNVAHSSYQGKGTADGVEPLKGSHRVLARIAEIHQNGKAMVEDSALVCRELVRCAVLWPELWFNELGRALYQWERQRSAENLLLAMGPLLEQLKRPETMAEAQFAAELRQPLENACCHVERAVSSRHEQFMEEARRIFVSIERRIREQISGMSSLALQLVSPKLHQNGRNLSLVVPGQYREDGNYPLIASFQNVLKVLNSKQRPRRIYINGSNGEIYKFLLKGHEDLRLDERVMQLLGFVNTILEKHSVPKRHDCLIQTYSVTPLSDNAGLVGWVDHCDTLNKIIEDYRVNPRCIRMELDLMRSMCDNLYYLTAIQRVEPFEFPLERTEGVDLVRSFWVKAPSAETWLERRTTYVCSLATMSMVGHILGLGDRHPSNLMIHAFSGRVVHIDFGDCFEVAQQRSIHPEKVPFRLTRMLVKAMEMGGIEGLFRHGCHTVMNVLREEGGSLLALLEAFVHDPLVSWWRDEAEGFSGNGQAASSVQTISVIGSMTNVAEEGSVGSLQLSQLSVQRRATTQQRVNANIEKDTSQTRKPKSVVKRIKRKLRGLEFPQSQEGKSSDGFTVEEQVSRLIEEATSNENLCVHFLGWCPFW DIP protein O76222 original sequence version: 67 q387i9_tryb2 Raptor_N domain-containing protein Tb11.03.0460 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MKRGTRIITPCRVVFSRLPIQSIMRNRRRVSVTSSTSALVNELLAAFAPHHLPSLSTGFDMGRSSEDIQDIDGHREWYRGGRGRRCPRLLLILCLNNGNDPPDFSRIDPCARLECWEEPYIARMPHIVTGGGSERIAALLQRQYHSQLQGAVSSMAVEVELEDAKKRMANIRLDAREELVVFHYNGHGMPRATGYGEIWMFDKKRTSYVPLSLAEMAEHLSSPTLYVFDCNSAGSLLKFWCEERLHETRPDDMFICACSAGAFLPLNPQLPADVLTSSLTTPLRMASEWYINFSHRKHLLPHVTAEMIRNVPGDISDERTPLGELQLILTAVTDIVAWCIVPSPLYCKLFRRDETTMVLFRNFLLADRLLREVGCVPQTHPPIPEEAHLHHAWDLWDYTLENVICQLPDLLTRDLVLNPNYTYKPSTFFEDQLMSFEVWLKSGDMREQPEELPSVLSALKRPQCCVAALTLLVTYLDSDVIAGKWAILCGILTHITSLLRNQELFLMASVLWMQVLRVDESLGTSELIKRRLGNRFIKVLTLTEKDLCVQTVERGQVAPLRALNNVNRRVGRGDTQASGNNSACPAQISCYLMEGVDLGRCKEIACYLFCQLLRHGGTTACVECWNGGLLNAAASLLESPSAEVRSWSCLVLAQLLFSLQFAKDFVSRKCTTHVGLFTHLLQDKSPIVRGSCVTLLSSLIGFRVSEAPGEHAVRRLQVEKTLLIKLRGMVYDASVIVRQELVLFACTVLFHYGPFLPSLQDTTAKTRYVVYMQEVGQCFPSWFLDEPDVQVKSMLNTSRPTANRCFNSKSVFENAPCAPADLTEPPWTPLAMDDIRSEDLPVLRGMVHDAALMLFSLYQACDKVMVTAALEKLGDNKRPESQRFTNESLRSMSSVVSANSTHFMSEAERARVTRNADNMQRIVLDLHDQKTEPPSMCTTAANRTRSLAVDGHSATYNANHSSGIPGAGDEKVNLDLVYAPHQILQSLLPCDQVICSTLRVLETAMVIATRNQYIYHVSYESYSVQETVHSFQVHLASPLHDILVINDASEQAGLLLVNQRGGYSLIRDCWEKTQGSPVEVAVFSACPPQRTVDIKSAYRSHNANLIYGGPIGYGGGTEIHILSLDEEQVIQQLKVSGDPTITSLSAHTTGRAVFAGCSDGLVRYYDDRQKQGFLGAVGSLRCGTTSYSPMEAVLGAGPVERDVAQLTIAMASRSAVYLYDTRKVNAPYLEVRVNELCDAVVPSSAAKPPPIRAFDAGTHTGMLGVLFADGTYTALNARGRPILRRAIQTRVQGPIFPGSLAVHPLRRVMSVGGEILVVH 12F8BAE200C761C9 pi3k-tbavo3-1 7 12 unspecified role unspecified role bait bait 9tryp Trypanosoma brucei 11 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9tryp Trypanosoma brucei 13 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9tryp Trypanosoma brucei association association tbtor2-tbfkbp12-1 6 10 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 9 unspecified role unspecified role bait bait gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association tbraptor-pi3k-1 1 12 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 13 unspecified role unspecified role bait bait 9tryp Trypanosoma brucei physical association physical association tbavo3-tbtor2-1 2 9 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 11 unspecified role unspecified role bait bait myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9tryp Trypanosoma brucei physical association physical association tbavo3-pi3k-1 4 12 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 9 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 11 unspecified role unspecified role bait bait myc tag myc tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9tryp Trypanosoma brucei association association tbtor2-tbfkbp12-2 3 10 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 9 unspecified role unspecified role bait bait 9tryp Trypanosoma brucei physical association physical association tbtor2-tbavo3-1 5 11 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9tryp Trypanosoma brucei 9 unspecified role unspecified role bait bait 9tryp Trypanosoma brucei physical association physical association tbraptor-pi3k-2 8 12 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei 13 unspecified role unspecified role bait bait myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9tryp Trypanosoma brucei physical association physical association