DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113390X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113394X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113392X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113395X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113393X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113391X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Krick S., Shi S., Ju W., Faul C., Tsai SY., Mundel P., Böttinger EP. DIP original experiment: DIP-113396X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease. htra2_mouse Serine protease HTRA2, mitochondrial Htra2 Omi Prss25 High temperature requirement protein A2 Omi stress-regulated endoprotease Serine proteinase OMI Serine protease 25 protein protein mouse Mus musculus Mouse MAALKAGRGANWSLRAWRALGGIFWRKPPLLAPDLRALLTSGTPDSQIWMTYGTPSLPAQVPEGFLASRADLTSRTPDLWARLNVGTSGSSDQEARRSPGSRRREWLAVAVGAGGAVVLLLWGWGRGLSTVLAAVPAPPPTSPRSQYNFIADVVEKTAPAVVYIEILDRHPFSGREVPISNGSGFVVASDGLIVTNAHVVADRRRVRVRLPSGDTYEAMVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQNNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSWFGTSGSQRRYIGVMMLTLTPSILIELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEKLAQNAEDVYEAVRTQSQLAVRIRRGSETLTLYVTPEVTE DIP protein Q9JIY5 original sequence version: 116 DIP protein Q9JIY5 original sequence version: 79 C1E77346FB8D75BD mp17l_mouse Mpv17-like protein Mpv17l protein protein mouse Mus musculus Mouse MASWWRAFPQAARRYPWPTNVLLYAGLFSAGDALQQRLRGGPADWRQTRRVATLAVTFHGNFNYVWLRLLERALPGRAPRTVLAKVLCDQTVGGPIALSAFYVGMSVLQGKDDIFLDLKQKFWNTYKSGLMYWPFVQLTNFSLVPVHWRTAYTGLCAFLWATFLCFSQQSGDGTLQSIFIFLRRKEASDKSPEK DIP protein Q99MS3 original sequence version: 29 q99ms3-1 Mpv17-like protein Mpv17l M-LP long protein protein mouse Mus musculus Mouse MASWWRAFPQAARRYPWPTNVLLYAGLFSAGDALQQRLRGGPADWRQTRRVATLAVTFHGNFNYVWLRLLERALPGRAPRTVLAKVLCDQTVGGPIALSAFYVGMSVLQGKDDIFLDLKQKFWNTYKSGLMYWPFVQLTNFSLVPVHWRTAYTGLCAFLWATFLCFSQQSGDGTLQSIFIFLRRKEASDKSPEK DIP protein Q99MS3-1 original sequence version: 29 q99ms3-3 Mpv17-like protein Mpv17l M-LP short protein protein mouse Mus musculus Mouse MSVLQGKDDIFLDLKQKFWNTYKSGLMYWPFVQLTNFSLVPVHWRTAYTGLCAFLWATFLCFSQQSGDGTLQSIFIFLRRKEASDKSPEK mpv17l-htra2-1 1 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 10 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association htra2-mpv17l-1 4 11 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association mpv17l-htra2-2 5 9 unspecified role unspecified role bait bait mouse Mus musculus Mouse 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association mpv17l-htra2-3 6 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association htra2-mpv17l-2 7 9 unspecified role unspecified role prey prey mouse Mus musculus Mouse 8 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association mpv17l-htra2-4 2 8 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role bait bait Q99MS3-1:p.[Asp112Ala;Ile114Ala] D112A/I114A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D112A/I114A'. Wed May 02 14:29:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp112ala,ile114ala' Tue Jun 30 16:40:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp112Ala;Ile114Ala]' Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association htra2-mpv17l-3 3 10 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association