DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. DIP original experiment: DIP-113350X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. DIP original experiment: DIP-113349X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. DIP original experiment: DIP-113347X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. DIP original experiment: DIP-113345X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. DIP original experiment: DIP-113346X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Structure of Vps75 and implications for histone chaperone function. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tang Y., Meeth K., Jiang E., Luo C., Marmorstein R. DIP original experiment: DIP-113348X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structure of Vps75 and implications for histone chaperone function. rt109_yeast Histone acetyltransferase RTT109 RTT109 KIM2 REM50 Regulator of Ty1 transposition protein 109 L1377 YLL002W KAT11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGKVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYLQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFTCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQAKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEEDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRAIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLAITMLKPRKKAKALPKT 17825D6EF97C4BB5 17825D6EF97C4BB5 vps75_yeast Vacuolar protein sorting-associated protein 75 N0890 YNL246W VPS75 protein protein yeast Saccharomyces cerevisiae Baker's yeast MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKIVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIFGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSEDDDGSLGEVDLPLSDEEPSSKKRKV 779F12CEA27B3E6B 779F12CEA27B3E6B vps75-1 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rtt109-vps75-6 4 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction vps75-2 6 8 unspecified role unspecified role unspecified role unspecified role Region 1-232 Region 1-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rtt109-vps75-7 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rtt109-vps75-8 3 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role prey prey Region 9-222 Region 9-222 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P53853:p.[Glu218Lys;Asp222Lys] E218K/D222K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D K certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E218K/D222K'. Wed May 02 15:36:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu218lys,asp222lys' Tue Jun 30 15:52:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu218Lys;Asp222Lys]' P53853:p.[Arg173Glu;Lys177Glu] R173E/K177E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R173E/K177E'. Wed May 02 16:05:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg173glu,lys177glu' Tue Jun 30 19:42:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg173Glu;Lys177Glu]' ecolx Escherichia coli direct interaction direct interaction rtt109-vps75-9 2 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction