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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Membrane curvature induced by Arf1-GTP is essential for vesicle formation.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="18689681" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="doi" dbAc="MI:0574" id="10.1073/pnas.0805182105" refType="primary-reference" refTypeAc="MI:0358"/>
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            <secondaryRef db="imex" dbAc="MI:0670" id="IM-9497" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Membrane curvature induced by Arf1-GTP is essential for vesicle formation.</attribute>
            <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
            <attribute name="publication year" nameAc="MI:0886">2008</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Beck R.,  Sun Z.,  Adolf F.,  Rutz C.,  Bassler J.,  Wild K.,  Sinning I.,  Hurt E.,  Brügger B.,  Béthune J.,  Wieland F.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-9497" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-113279X" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="18689681" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="doi" dbAc="MI:0574" id="10.1073/pnas.0805182105" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>crosslink</shortLabel>
            <fullName>cross-linking study</fullName>
            <alias type="synonym" typeAc="MI:1041">crosslink</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0030" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1049" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Beck R., Sun Z., Adolf F., Rutz C., Bassler J., Wild K., Sinning I., Hurt E., Brügger B., Béthune J., Wieland F.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-113279X</attribute>
          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2008</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Membrane curvature induced by Arf1-GTP is essential for vesicle formation.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>arf1_human</shortLabel>
          <fullName>ADP-ribosylation factor 1</fullName>
          <alias type="gene name" typeAc="MI:0301">ARF1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P84077" version="SP_44" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P10947" version="SP_44" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P32889" version="SP_44" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000272102" version="SP_190" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000440005" version="SP_190" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000442980" version="SP_190" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-447171" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001019397.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001019398.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001019399.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001649.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-31597N"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSG00000143761" version="SP_190" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000272102" version="SP_190" refType="transcript"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000540651" version="SP_190" refType="transcript"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000541182" version="SP_190" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000139" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000287" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0002090" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003723" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003924" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005525" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005770" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005778" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005802" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005829" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" version="SP_190"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007015" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0014069" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016192" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019003" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019904" version="SP_190"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032991" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0034315" version="SP_190"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0097061" version="SP_190"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:1990386" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1990583" version="SP_190"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005225" version="SP_190"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1902953" version="SP_190"/>
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          <secondaryRef db="mint" dbAc="MI:0471" id="P84077" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1HUR" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1RE0" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1U81" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3O47" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4HMY" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6CM9" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6CRI" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6D83" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6D84" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6DFF" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6FAE" version="SP_190"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7MGE" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-1660499" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-1660514" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-167590" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-199992" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-2132295" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-432720" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-432722" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-6807878" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-6811434" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-6811438" version="SP_190"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-8950505" version="SP_190"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P84077 original sequence version: 122</attribute>
          <attribute name="remark-internal">DIP protein P84077 original sequence version: 56</attribute>
          <attribute name="remark-internal">DIP protein P84077 original sequence version: 89</attribute>
          <attribute name="crc64">AAC773D4A60186B6</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-9497-1">
        <names>
          <shortLabel>arf1-5</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-88169E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15720705" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-9497-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>