DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Nature 2008 imex curation Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. DIP original experiment: DIP-250342X Nature 2008 IMEx Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Nature 2008 imex curation Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. DIP original experiment: DIP-250340X Nature 2008 IMEx Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Nature 2008 imex curation Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. DIP original experiment: DIP-250341X Nature 2008 IMEx Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Nature 2008 imex curation Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. DIP original experiment: DIP-250339X Nature 2008 IMEx Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). Nature 2008 imex curation Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Cheng HC., Skehan BM., Campellone KG., Leong JM., Rosen MK. DIP original experiment: DIP-250343X Nature 2008 IMEx Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U). wasp_human Wiskott-Aldrich syndrome protein WAS IMD2 protein protein human Homo sapiens Human MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD DIP protein P42768 original sequence version: 183 DIP protein P42768 original sequence version: 168 DIP protein P42768 original sequence version: 132 DIP protein P42768 original sequence version: 155 7228428672B7CB78 wasl_human Neural Wiskott-Aldrich syndrome protein WASL protein protein human Homo sapiens Human MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED DIP protein O00401 original sequence version: 55 DIP protein O00401 original sequence version: 147 DIP protein O00401 original sequence version: 150 DIP protein O00401 original sequence version: 136 DIP protein O00401 original sequence version: 138 DIP protein O00401 original sequence version: 125 DIP protein O00401 original sequence version: 112 DIP protein O00401 original sequence version: 126 B5895C6055E23049 esfu2_eco57 Secreted effector protein EspF(U) espF(U) tccP EspF-like protein encoded on prophage U Tir-cytoskeleton coupling protein TccP ECs2715 protein protein eco57 Escherichia coli O157:H7 MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFSPSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSMAEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWPAPPLPVQNEQSRPLPDVAQRLVQHLAEHGIQPARSMAEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLMQHLAEHGIQPARNMAEHIPPAPNWPAPTPPVQNEQSRPLPDVAQRLMQHLAEHGIQPARNMAEHIPPAPNWPAPTPPVQNEQSRPLPDVAQRLMQHLAEHGINTSKRS DIP protein P0DJ89 original sequence version: 5 This sequence has been withdrawn from Uniprot. 49A1BE2EA220E1C0 was-espfu-1 3 8 unspecified role unspecified role prey prey Region 268-300 Region 268-300 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 242-310 Region 242-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction espfu-was-1 4 8 unspecified role unspecified role unspecified role unspecified role Region 268-300 Region 268-300 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 242-310 Region 242-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espfu-wasl-4 5 7 unspecified role unspecified role unspecified role unspecified role Region 193-501 Region 193-501 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 268-314 Region 268-314 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espfu-was-2 2 8 unspecified role unspecified role unspecified role unspecified role Region 268-300 Region 268-300 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 242-310 Region 242-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction was-was-4 1 6 unspecified role unspecified role prey prey Region 420-502 Region 420-502 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 242-310 Region 242-310 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction