DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tian J., Kim SF., Hester L., Snyder SH. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tian J., Kim SF., Hester L., Snyder SH. DIP original experiment: DIP-111264X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tian J., Kim SF., Hester L., Snyder SH. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tian J., Kim SF., Hester L., Snyder SH. DIP original experiment: DIP-111263X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tian J., Kim SF., Hester L., Snyder SH. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Tian J., Kim SF., Hester L., Snyder SH. DIP original experiment: DIP-111265X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Tian J., Kim SF., Hester L., Snyder SH. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tian J., Kim SF., Hester L., Snyder SH. DIP original experiment: DIP-111266X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx S-nitrosylation/activation of COX-2 mediates NMDA neurotoxicity. nos1_mouse Nitric oxide synthase, brain Nos1 bNOS NOS type I Neuronal NOS Constitutive NOS NC-NOS Peptidyl-cysteine S-nitrosylase NOS1 protein protein mouse Mus musculus Mouse MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS DIP protein Q9Z0J4 original sequence version: 144 DIP protein Q9Z0J4 original sequence version: 90 3782848D65B41BFC pgh2_mouse Prostaglandin G/H synthase 2 Ptgs2 Cox-2 Cox2 Pghs-b Tis10 Gripghs TIS10 protein Macrophage activation-associated marker protein P71/73 PES-2 Cyclooxygenase-2 Prostaglandin-endoperoxide synthase 2 Prostaglandin H2 synthase 2 PHS II Glucocorticoid-regulated inflammatory cyclooxygenase protein protein mouse Mus musculus Mouse MLFRAVLLCAALGLSQAANPCCSNPCQNRGECMSTGFDQYKCDCTRTGFYGENCTTPEFLTRIKLLLKPTPNTVHYILTHFKGVWNIVNNIPFLRSLIMKYVLTSRSYLIDSPPTYNVHYGYKSWEAFSNLSYYTRALPPVADDCPTPMGVKGNKELPDSKEVLEKVLLRREFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPGFTRGLGHGVDLNHIYGETLDRQHKLRLFKDGKLKYQVIGGEVYPPTVKDTQVEMIYPPHIPENLQFAVGQEVFGLVPGLMMYATIWLREHNRVCDILKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNQQFQYQNRIASEFNTLYHWHPLLPDTFNIEDQEYSFKQFLYNNSILLEHGLTQFVESFTRQIAGRVAGGRNVPIAVQAVAKASIDQSREMKYQSLNEYRKRFSLKPYTSFEELTGEKEMAAELKALYSDIDVMELYPALLVEKPRPDAIFGETMVELGAPFSLKGLMGNPICSPQYWKPSTFGGEVGFKIINTASIQSLICNNVKGCPFTSFNVQDPQPTKTATINASASHSRLDDINPTVLIKRRSTEL not sure if the protein origin is right determined, my suggestion is based on fig.1c and 1d description DIP protein Q05769 original sequence version: 98 DFE1658295C92064 ptgs2-nos1-1 2 5 unspecified role unspecified role prey prey mouse Mus musculus Mouse 6 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association ptgs2-nos1-2 1 5 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait binding site 594-604 binding site 594-604 required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association ptgs2-nos1-3 4 5 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association ptgs2-nos1-4 3 5 unspecified role unspecified role prey prey binding site 17-99 binding site 17-99 required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 6 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association