DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 2008 imex curation Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. DIP original experiment: DIP-250235X Nature 2008 IMEx Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 2008 imex curation Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. DIP original experiment: DIP-250237X Nature 2008 IMEx Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 2008 imex curation Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. DIP original experiment: DIP-250240X Nature 2008 IMEx Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 2008 imex curation Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. DIP original experiment: DIP-250236X Nature 2008 IMEx Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 2008 imex curation Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. DIP original experiment: DIP-250239X Nature 2008 IMEx Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. Nature 2008 imex curation Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Macůrek L., Lindqvist A., Lim D., Lampson MA., Klompmaker R., Freire R., Clouin C., Taylor SS., Yaffe MB., Medema RH. DIP original experiment: DIP-250238X Nature 2008 IMEx Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery. bora_human Protein aurora borealis BORA C13orf34 protein protein human Homo sapiens Human MGDVKESKMQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSVFKSTKLPTPGKFRWSIDQLAVINPVEIDPEDIHRQALYLSHSRIDKDVEDKRQKAIEEFFTKDVIVPSPWTDHEGKQLSQCHSSKCTNINSDSPVGKKLTIHSEKSDAACQTLLSLPVDFNLENILGDYFRADEFADQSPGNLSSSSLRRKLFLDGNGSISDSLPSASPGSPHSGVQTSLEMFYSIDLSPVKCRSPLQTPSSGQFSSSPIQASAKKYSLGSITSPSPISSPTFSPIEFQIGETPLSEQRKFTVHSPDASSGTNSNGITNPCIRSPYIDGCSPIKNWSPMRLQMYSGGTQYRTSVIQIPFTLETQGEDEEDKENIPSTDVSSPAMDAAGIHLRQFSNEASTHGTHLVVTAMSVTQNQSSASEKELALLQDVEREKDNNTVDMVDPIEIADETTWIKEPVDNGSLPMTDFVSGIAFSIENSHMCMSPLAESSVIPCESSNIQMDSGYNTQNCGSNIMDTVGAESYCKESDAQTCEVESKSQAFNMKQDHTTQRCWMKTASPFQCSSP DIP protein Q6PGQ7 original sequence version: 70 F817E6DC2C7600DB plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS DIP protein O14965 original sequence version: 181 DIP protein O14965 original sequence version: 146 DIP protein O14965 original sequence version: 124 DIP protein O14965 original sequence version: 160 DIP protein O14965 original sequence version: 138 DIP protein O14965 original sequence version: 98 125F3594834CD157 aurka-plk1-1 1 9 enzyme enzyme unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pT210 pT210 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction aurka-bora-1 2 7 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role prey prey human Homo sapiens Human association association bora-plk1-1 5 7 unspecified role unspecified role bait bait EGFP-tag EGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 8 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association aurka-plk1-2 4 9 enzyme enzyme unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O14965:p.Lys162Arg K162R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K162R'. Wed May 02 16:45:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys162arg' Tue Jun 30 18:01:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys162Arg' ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pT210 pT210 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction aurka-plk1-3 6 9 enzyme enzyme unspecified role unspecified role human Homo sapiens Human 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pT210 pT210 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction plk1-bora-1 3 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association