DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113618X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113614X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113617X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113613X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113619X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113615X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nature structural & molecular biology 2008 imex curation Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Katayama H., Janowiak BE., Brzozowski M., Juryck J., Falke S., Gogol EP., Collier RJ., Fisher MT. DIP original experiment: DIP-113616X Nature structural & molecular biology 2008 IMEx GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. pag_bacan Protective antigen PA-83 Anthrax toxins translocating protein pagA pag pXO1-110 BXA0164 GBAA_pXO1_0164 protein protein bacan Bacillus anthracis MKKRKVLIPLMALSTILVSSTGNLEVIQAEVKQENRLLNESESSSQGLLGYYFSDLNFQAPMVVTSSTTGDLSIPSSELENIPSENQYFQSAIWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGRLYQIKIQYQRENPTEKGLDFKLYWTDSQNKKEVISSDNLQLPELKQKSSNSRKKRSTSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLATIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDGKTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIG DIP protein P13423 original sequence version: 106 DIP protein P13423 original sequence version: 91 DIP protein P13423 original sequence version: 93 DIP protein P13423 original sequence version: 110 DIP protein P13423 original sequence version: 124 DIP protein P13423 original sequence version: 150 3AE1EFBF48FAA03F ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 CD3A0FB505F74AD1 lef_bacan Lethal factor Anthrax lethal toxin endopeptidase component lef pXO1-107 BXA0172 GBAA_pXO1_0172 protein protein bacan Bacillus anthracis MNIKKEFIKVISMSCLVTAITLSGPVFIPLVQGAGGHGDVGMHVKEKEKNKDENKRKDEERNKTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVPSDVLEMYKAIGGKIYIVDGDITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVLVIQSSEDYVENTEKALNVYYEIGKILSRDILSKINQPYQKFLDVLNTIKNASDSDGQDLLFTNQLKEHPTDFSVEFLEQNSNEVQEVFAKAFAYYIEPQHRDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLARYEKWEKIKQHYQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSDFLSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKLDIQPYDINQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGSTLYNKIYLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMIVDINERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEIKDVQIIKQSEKEYIRIDAKVVPKSKIDTKIQEAQLNINQEWNKALGLPKYTKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNGRFVFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFIHEFGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAEFFAEAFRLMHSTDHAERLKVQKNAPKTFQFINDQIKFIINS DIP protein P15917 original sequence version: 109 DIP protein P15917 original sequence version: 138 DIP protein P15917 original sequence version: 93 DIP protein P15917 original sequence version: 98 DIP protein P15917 original sequence version: 112 DIP protein P15917 original sequence version: 125 2076B4D7277317EE paga-4 4 8 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Region 197-764 Region 197-764 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lef-paga-5 7 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction groel-paga-1 5 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction paga-5 2 8 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Region 197-764 Region 197-764 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction groel-4 3 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction groel-paga-2 6 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction paga-6 1 8 unspecified role unspecified role unspecified role unspecified role Region 197-764 Region 197-764 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Region 197-764 Region 197-764 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction