DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110842X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110843X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110849X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110848X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110846X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110844X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110845X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS biology 2008 imex curation Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Miller JL., Le Coq J., Hodes A., Barbalat R., Miller JF., Ghosh P. DIP original experiment: DIP-110847X PLoS biology 2008 IMEx Selective ligand recognition by a diversity-generating retroelement variable protein. pert_borbr Pertactin autotransporter P.94 BB1366 prn protein protein borbr Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) Alcaligenes bronchisepticus MNMSLSRIVKAAPLRRTTLAMALGALGALGAAPAAHADWNNQSIIKAGERQHGIHIKQSDGAGVRTATGTTIKVSGRQAQGVLLENPAAELRFQNGSVTSSGQLFDEGVRRFLGTVTVKAGKLVADHATLANVSDTRDDDGIALYVAGEQAQASIADSTLQGAGGVRVERGANVTVQRSTIVDGGLHIGTLQPLQPEDLPPSRVVLGDTSVTAVPASGAPAAVSVFGANELTVDGGHITGGRAAGVAAMDGAIVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPLLDGWYGVDVSDSTVDLAQSIVEAPQLGAAIRAGRGARVTVSGGSLSAPHGNVIETGGGARRFPPPASPLSITLQAGARAQGRALLYRVLPEPVKLTLAGGAQGQGDIVATELPPIPGASSGPLDVALASQARWTGATRAVDSLSIDNATWVMTDNSNVGALRLASDGSVDFQQPAEAGRFKVLMVDTLAGSGLFRMNVFADLGLSDKLVVMRDASGQHRLWVRNSGSEPASANTMLLVQTPRGSAATFTLANKDGKVDIGTYRYRLAANGNGQWSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRLNPDAGGAWGRGFAQRQQLDNRAGRRFDQKVAGFELGADHAVAVAGGRWHLGGLAGYTRGDRGFTGDGGGHTDSVHVGGYATYIANSGFYLDATLRASRLENDFKVAGSDGYAVKGKYRTHGVGASLEAGRRFAHADGWFLEPQAELAVFRVGGGAYRAANGLRVRDEGGSSVLGRLGLEVGKRIELAGGRQVQPYIKASVLQEFDGAGTVRTNGIAHRTELRGTRAELGLGMAAALGRGHSLYASYEYSKGPKLAMPWTFHAGYRYSW DIP protein Q03035 original sequence version: 107 mtdm1 hypothetical protein predicted by GeneMark protein protein bordetella phage bmp-1 Bordetella phage BMP-1 mtdp6 Phage tail protein protein protein bpp1virus Bpp1virus MSTAVQFRGGTTAQHATFTGAAREITVDTDKNTVVVHDGATAGGFPLARHDLVKTAFIKADKSAVAFTRTGNATASIKAGTIVEVNGKLVQFTADTAITMPALTAGTDYAIYVCDDGTVRADSNFSAPTGYTSTTARKVGGFHYAPGSNAAAQAGGNTTAQINEYSLWDIKFRPAALDPRGMTLVAGAFWADIYLLGVNHLTDGTSKYNVTIADGSASPKKSTKFGGDGSAAYSDGAWYNFAEVMTHHGKRLPNYNEFQALAFGTTEATSSGGTDVPTTGVNGTGATSAWNIFTSKWGVVQASGCLWTWGNEFGGVNGASEYTANTGGRGSVYAQPAAALFGGSWHYTSNSGSRAAIWYYGPSISNASIGARGVCDHLILE fibd_bpbpp Tail fiber receptor-binding protein Major tropism determinant protein mtd protein protein bpbpp Bordetella phage BPP-1 MSTAVQFRGGTTAQHATFTGAAREITVDTDKNTVVVHDGATAGGFPLARHDLVKTAFIKADKSAVAFTRTGNATASIKAGTIVEVNGKLVQFTADTAITMPALTAGTDYAIYVCDDGTVRADSNFSAPTGYTSTTARKVGGFHYAPGSNAAAQAGGNTTAQINEYSLWDIKFRPAALDPRGMTLVAGAFWADIYLLGVNHLTDGTSKYNVTIADGSASPKKSTKFGGDGSAAYSDGAWYNFAEVMTHHGKRLPNYNEFQALAFGTTEATSSGGTDVPTTGVNGTGATSAWNIFTSKWGVVQASGCLWTWGNEFGGVNGASEYTANTGGRGSVYAQPAAALFGGAWNGTSLSGSRAALWYSGPSFSFAFFGARGVCDHLILE DIP protein Q775D6 original sequence version: 38 DIP protein Q775D6 original sequence version: 17 mtd-prn_e-1 1 9 unspecified role unspecified role unspecified role unspecified role Region 38-640 Region 38-640 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction prn_e-mtd-1 5 9 unspecified role unspecified role bait bait Region 38-640 Region 38-640 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction prn_e-mtdm1-1 8 10 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait Region 38-640 Region 38-640 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction prn_e-mtdp6-1 3 11 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 38-640 Region 38-640 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mtd-prn_e-2 2 9 unspecified role unspecified role unspecified role unspecified role Region 38-640 Region 38-640 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction prn_e-mtdm1-2 7 10 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait Region 190-199 Region 190-199 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction prn_e-mtdp6-2 4 11 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait Region 38-640 Region 38-640 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction prn_e-mtd-2 6 9 unspecified role unspecified role bait bait Region 399-407 Region 399-407 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 190-199 Region 190-199 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction